Graded reduction of Cdc20 in MEFs induces progressive aneuploidy
| Mitotic MEF genotypea | Aneuploid figs. | Karyotypes w/ indicated chromosome No. | Mitotic figs. w/ PMSCS | Mitotic figs. w/ PMSCS in MG132 | ||||||
| 37 | 38 | 39 | 40 | 41 | 42 | 43 | ||||
| % | % | |||||||||
| +/+ | 12 (2) | 1 | 2 | 5 | 132 | 7 | 3 | NA | 3 (1) | 0 (0) |
| Cdc20+/− | 17 (1) | 1 | NA | 11 | 124 | 10 | 4 | NA | 10 (1) | NA |
| Cdc20H/H | 23 (2) | NA | 5 | 9 | 115 | 18 | 3 | NA | 11 (2) | NA |
| Cdc20−/H | 27 (1) | 3 | 4 | 9 | 110 | 14 | 7 | 3 | 12 (1) | 0 (0) |
| Mitotic MEF genotypea | Aneuploid figs. | Karyotypes w/ indicated chromosome No. | Mitotic figs. w/ PMSCS | Mitotic figs. w/ PMSCS in MG132 | ||||||
| 37 | 38 | 39 | 40 | 41 | 42 | 43 | ||||
| % | % | |||||||||
| +/+ | 12 (2) | 1 | 2 | 5 | 132 | 7 | 3 | NA | 3 (1) | 0 (0) |
| Cdc20+/− | 17 (1) | 1 | NA | 11 | 124 | 10 | 4 | NA | 10 (1) | NA |
| Cdc20H/H | 23 (2) | NA | 5 | 9 | 115 | 18 | 3 | NA | 11 (2) | NA |
| Cdc20−/H | 27 (1) | 3 | 4 | 9 | 110 | 14 | 7 | 3 | 12 (1) | 0 (0) |
150 mitotic figures were inspected. NA, not applicable. Numbers in parentheses indicate SD. Karyotyping was performed at passage 5. MG132 treatment was for 2 h in 10 µM of the drug.
n = 3.