Plasmids used in this study
| Plasmid | Description | Reference or source |
| pUG23-MYO1 | CEN, HIS3, pMET25-yEGFP3, MYO1 | See text |
| pRS316-N-MYO1-GFP | CEN, URA3, GFP-MYO1 | (Caviston et al., 2003) |
| pRS316-MYO1-C-GFP | CEN, URA3, MYO1-GFP | See text |
| pRS316-MYO1-TD2 -GFP | CEN, URA3, myo1-TD2-GFP | See text |
| pRS316-MYO1-TD2Δ-GFP | CEN, URA3, myo1-TD2Δ-GFP | See text |
| pRS316-MYO1-TD1-GFP | CEN, URA3, myo1-TD1-GFP | See text |
| pRS316-MYO1-mTD1-GFP | CEN, URA3, myo1-mTD1-GFP | See text |
| pUC18-C-TAP | C-TAP | See text |
| pRS316-MYO1-C-TAP | CEN, URA3, MYO1-C-TAP | See text |
| pRS316-MYO1-TD2Δ-C-TAP | CEN, URA3, MYO1-TD2Δ-C-TAP | See text |
| pRS316-MYO1-mCherry | CEN, URA3, MYO1-mCherry | See text |
| pMAL-c2 | malE (or N-MBP) | New England Biolabs |
| pMAL-MYO1-TD1 | MBP-myo1-TD1 | See text |
| pMAL-MYO1-mTD1 | MBP-myo1-mTD1 | See text |
| pMAL-MYO1-TD2 | MBP-myo1-TD2 | See text |
| pGEX-5X-BNI5 | GST-BNI5 | See text |
| YIplac128-CDC3-mCherry | Integrative, LEU2, mCherry (RFP)-CDC3 | (Gao et al., 2007) |
| YIplac211-CDC3-mCherry | Integrative, URA3, mCherry (RFP)-CDC3 | See text |
| pRS315-IQG1 | CEN, LEU2, IQG1 | See text |
| pRS315-IQG1-GST | CEN, LEU2, IQG1-GST | See text |
| pRS315-IQG1-(1-697)-GST | CEN, LEU2, iqg1(1-697)-GST | See text |
| pRS315-IQG1(Δ2-411)-GST | CEN, LEU2, iqg1(1, 412-1495)-GST | See text |
| YCp50-IQG1 | CEN, URA3, IQG1 | (Korinek et al., 2000) |
| Plasmid | Description | Reference or source |
| pUG23-MYO1 | CEN, HIS3, pMET25-yEGFP3, MYO1 | See text |
| pRS316-N-MYO1-GFP | CEN, URA3, GFP-MYO1 | (Caviston et al., 2003) |
| pRS316-MYO1-C-GFP | CEN, URA3, MYO1-GFP | See text |
| pRS316-MYO1-TD2 -GFP | CEN, URA3, myo1-TD2-GFP | See text |
| pRS316-MYO1-TD2Δ-GFP | CEN, URA3, myo1-TD2Δ-GFP | See text |
| pRS316-MYO1-TD1-GFP | CEN, URA3, myo1-TD1-GFP | See text |
| pRS316-MYO1-mTD1-GFP | CEN, URA3, myo1-mTD1-GFP | See text |
| pUC18-C-TAP | C-TAP | See text |
| pRS316-MYO1-C-TAP | CEN, URA3, MYO1-C-TAP | See text |
| pRS316-MYO1-TD2Δ-C-TAP | CEN, URA3, MYO1-TD2Δ-C-TAP | See text |
| pRS316-MYO1-mCherry | CEN, URA3, MYO1-mCherry | See text |
| pMAL-c2 | malE (or N-MBP) | New England Biolabs |
| pMAL-MYO1-TD1 | MBP-myo1-TD1 | See text |
| pMAL-MYO1-mTD1 | MBP-myo1-mTD1 | See text |
| pMAL-MYO1-TD2 | MBP-myo1-TD2 | See text |
| pGEX-5X-BNI5 | GST-BNI5 | See text |
| YIplac128-CDC3-mCherry | Integrative, LEU2, mCherry (RFP)-CDC3 | (Gao et al., 2007) |
| YIplac211-CDC3-mCherry | Integrative, URA3, mCherry (RFP)-CDC3 | See text |
| pRS315-IQG1 | CEN, LEU2, IQG1 | See text |
| pRS315-IQG1-GST | CEN, LEU2, IQG1-GST | See text |
| pRS315-IQG1-(1-697)-GST | CEN, LEU2, iqg1(1-697)-GST | See text |
| pRS315-IQG1(Δ2-411)-GST | CEN, LEU2, iqg1(1, 412-1495)-GST | See text |
| YCp50-IQG1 | CEN, URA3, IQG1 | (Korinek et al., 2000) |