Rescue of MOR pathway mutants by the Nak1–Orb6 fusion
Strain
Vector
Nak1
Orb6
Nak1–Orb6 fusion
pmo25-35
−
−
N/A
−
nak1-167
−
+
−
+
orb6-25
−
N/A
+
+
mor2-786
−
−
−
+
Strain
Vector
Nak1
Orb6
Nak1–Orb6 fusion
pmo25-35
−
−
N/A
−
nak1-167
−
+
−
+
orb6-25
−
N/A
+
+
mor2-786
−
−
−
+
The ability of the Nak1–Orb6 fusion to rescue (+) or not rescue (−) MOR pathway mutants at the restrictive temperature of 36°C is shown. N/A indicates condition that was not tested.