Size, expression characteristics, and nuclear position of BAC transgenes in ES cells
| BAC clone | Insert copy number/size estimate | Expression relative to the endogenous locusa | Cells with LacI signal touching the nuclear periphery | Mean/median distance of LacI signal from nuclear periphery |
| % | % | µm | ||
| β-Globin 2b | 893 (205 Mb) | NA | 84 (n = 32) | 0.053/0.00 |
| β-Globin 8d | 158 (36.3 Mb) | NA | 78 (n = 27) | 0.075/0.00 |
| β-Globin 3f | 54 (12.4 Mb) | NA | 52 (n = 27) | 0.101/0.00 |
| α-Globin 1c | 609 (115 Mb) | 11.8 | 16 (n = 25) | 0.34/0.35 |
| α-Globin 7d | 105 (20 Mb) | 6.6 | 26 (n = 23) | 0.323/0.312 |
| α-Globin 5c | 56 (12.5 Mb) | 7.3 | 4 (n = 23) | 0.58/0.485 |
| Dhfr 36 | 278 (52.8 Mb) | 52.8 | 16 (n = 32) | 0.359/0.261 |
| Dhfr 44 | 271 (51.5 Mb) | 35.3 | 7 (n = 28) | 0.340/0.377 |
| Dhfr 33 | 111 (21 Mb) | 11.1 | 15 (n = 27) | 0.445/0.384 |
| BAC clone | Insert copy number/size estimate | Expression relative to the endogenous locusa | Cells with LacI signal touching the nuclear periphery | Mean/median distance of LacI signal from nuclear periphery |
| % | % | µm | ||
| β-Globin 2b | 893 (205 Mb) | NA | 84 (n = 32) | 0.053/0.00 |
| β-Globin 8d | 158 (36.3 Mb) | NA | 78 (n = 27) | 0.075/0.00 |
| β-Globin 3f | 54 (12.4 Mb) | NA | 52 (n = 27) | 0.101/0.00 |
| α-Globin 1c | 609 (115 Mb) | 11.8 | 16 (n = 25) | 0.34/0.35 |
| α-Globin 7d | 105 (20 Mb) | 6.6 | 26 (n = 23) | 0.323/0.312 |
| α-Globin 5c | 56 (12.5 Mb) | 7.3 | 4 (n = 23) | 0.58/0.485 |
| Dhfr 36 | 278 (52.8 Mb) | 52.8 | 16 (n = 32) | 0.359/0.261 |
| Dhfr 44 | 271 (51.5 Mb) | 35.3 | 7 (n = 28) | 0.340/0.377 |
| Dhfr 33 | 111 (21 Mb) | 11.1 | 15 (n = 27) | 0.445/0.384 |
NA, not applicable.
Based on reverse transcription quantitative PCR data for C16orf33 (α-globin clones) and Dhfr (Dhfr clones).