Table II.

Quantification of chromatid segregation patterns in solo, snm, solo; snm, and controls

Chromatids WT solo snm solo; snm 
Anaphase I     
XX\YY 46 (100%) 23 (31%) 41 (58%) 13 (14%) 
XY\XY 48 (65%) 41 (43%) 
XXY\Y 2 (2.7%) 4 (5.6%) 19 (20%) 
XYY\X 1 (1.3%) 4 (5.6%) 16 (17%) 
XXYY\O 22 (31%) 6 (6%) 
Total 46 74 71 95 
Metaphase II     
XX 67 (52%) 15 (20%) ND ND 
YY 61 (48%) 14 (18%) ND ND 
XY 47 (62%) ND ND 
X or XYY ND ND 
Y or XXY ND ND 
O or XXYY ND ND 
Total 128 76 ND ND 

Genotypes: WT, yw; solo, soloZ2-0198/Df(2L)A267; snm, snmZ3-2138/snmZ3-0317; and solo; snm, soloZ2-0198/soloZ2-3534; snmZ3-2138/snmZ3-0317. Chromatid constitution was determined primarily by numbers of 359 bp and AATAC repeat spots per pole or nucleus. For 359 bp repeat spots in WT and snm, some poles or nuclei exhibited only one 359 bp spot as a result of cohesion. In these cases, the karyotype determination was based on the number of X chromatids per chromosome revealed by DAPI staining. Percentages of each karyotype within each genotype are shown in parentheses.

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