Phospholipid distribution in the membrane single-channel experiments
| Compound | CMC | Nominal [PL] | [PL]a | {PL}m | Mole %PL | ||||
| LUV | Planar bilayer | LUV | Planar bilayer | LUV | Planar bilayer | LUV | Planar bilayer | ||
| μM | μM | μM | μM | μM | moles/cm2 | moles/cm2 | mole % | mole % | |
| diC8PC | 270a | 30 | 3 | 15 | 1 | 2 × 10−11 | 1 × 10−12 | 6 | 0.4 |
| diC8PE | 230b | 30 | 3 | 10 | 1 | 2 × 10−11 | 1 × 10−12 | 8 | 0.4 |
| diC8PI | 500c | 30 | 3 | 20 | 1.5 | 1 × 10−11 | 7 × 10−13 | 4 | 0.3 |
| diC8PS | 500a | 30 | 3 | 20 | 1.5 | 1 × 10−11 | 7 × 10−13 | 4 | 0.3 |
| brain PIP2 | 30d | 50 | 50 | 5 | 3 | 4 × 10−11 | 2 × 10−11 | 15 | 9.0 |
| Compound | CMC | Nominal [PL] | [PL]a | {PL}m | Mole %PL | ||||
| LUV | Planar bilayer | LUV | Planar bilayer | LUV | Planar bilayer | LUV | Planar bilayer | ||
| μM | μM | μM | μM | μM | moles/cm2 | moles/cm2 | mole % | mole % | |
| diC8PC | 270a | 30 | 3 | 15 | 1 | 2 × 10−11 | 1 × 10−12 | 6 | 0.4 |
| diC8PE | 230b | 30 | 3 | 10 | 1 | 2 × 10−11 | 1 × 10−12 | 8 | 0.4 |
| diC8PI | 500c | 30 | 3 | 20 | 1.5 | 1 × 10−11 | 7 × 10−13 | 4 | 0.3 |
| diC8PS | 500a | 30 | 3 | 20 | 1.5 | 1 × 10−11 | 7 × 10−13 | 4 | 0.3 |
| brain PIP2 | 30d | 50 | 50 | 5 | 3 | 4 × 10−11 | 2 × 10−11 | 15 | 9.0 |
The aqueous phospholipid concentrations and their surface densities and mole percentage in the membrane ([PL]a, {PL}m, and mole %PL, respectively) were calculated according to Bruno et al. (2007) and Rusinova et al. (2011) using 1.2/CMC as an estimate for the (dimensionless) partition coefficient into the membrane (Bullock and Cohen, 1986); the total phospholipid concentration in the membrane was taken to be 1.2 M. This may overestimate the partition coefficient and mole %PL, as Heerklotz and Seelig (2000) found the partition coefficient for diC7PC to be threefold less than predicted using the above estimator. (The difference between the mole fractions in the LUVs vs. the planar bilayer is a result of the different amount of lipids in the different systems.)
Zhou et al., 1997.
Estimated from the CMC for diC6PE, 12 mM (Zhou et al., 1997), and scaling by the ratio of the CMC for diC8PC over the CMC for diC6PC.
Wang et al., 2008.
Palmer, 1981; Huang and Huang, 1991; Moens and Bagatolli, 2007.