Table 1. Primers for PCR shown in Figs.... | Rockefeller University Press

Table 1.

Primers for PCR shown in Figs. 1 and S1

	Name	Sequence (5′ to 3′)	Annealing
a	F-GORASP2 ex4	CCGATCCTTTCCTCAGCCGTCAGC	62.0°C
b	R-GORASP2 ex4LoxP	TGTGGAGTCCTTCCTTGCTC	62.0°C
c	F-GORASP2 ex3	GGGTTAGCATTCGTTTCTGC	53.4°C
d	R-GORASP2 ex5	CATGCAGAGTTTGGTGTGATG	53.4°C
e	F-GORASP2 int5	GAGCAAGGAAGGACTCCACA	56.8°C
f	R-GORASP2 int5	CAAGAGGCCGAGATCCTGAG	56.8°C
g	F-GORASP2 ex6	CGAATACCTACACGCCCCTT	57.2°C
h	R-GORASP2 ex7	TGCCGAGCTAATGGAGAGTC	57.2°C
i	F-GORASP2 ex8	ACTGTACCATTGCCGCC	58.4°C
j	R-GORASP2 ex9	GAGCTCTGGCAAACCGACC	58.4°C
k	F-GORASP2 ex4	AATCCAATTCCCCAGCG	57.7°C
l	R-GORASP2 ex6	GGGGGTACCAGTCATTTGTCC	57.7°C
m	F-GORASP2 ex1	CAAGTGCCACGTCCCAAGAG	58.5°C
n	R-GORASP2 ex4	CTGTGAGGTCTCAATCCTGCC	58.5°C
o	F-GORASP2 ex6	CGAATACCTACACGCCCCTT	57.2°C
p	R-GORASP2 ex10	ATGCCAGGTAAGTTTCGGGG	57.2°C
	F-GAPDH ex3–4	TGTCGTGGAGTCTACTGGTG	53.1°C
	R-GAPDH ex3–4	ACACCCATCACAAACATGG	53.1°C

	Name	Sequence (5′ to 3′)	Annealing
a	F-GORASP2 ex4	CCGATCCTTTCCTCAGCCGTCAGC	62.0°C
b	R-GORASP2 ex4LoxP	TGTGGAGTCCTTCCTTGCTC	62.0°C
c	F-GORASP2 ex3	GGGTTAGCATTCGTTTCTGC	53.4°C
d	R-GORASP2 ex5	CATGCAGAGTTTGGTGTGATG	53.4°C
e	F-GORASP2 int5	GAGCAAGGAAGGACTCCACA	56.8°C
f	R-GORASP2 int5	CAAGAGGCCGAGATCCTGAG	56.8°C
g	F-GORASP2 ex6	CGAATACCTACACGCCCCTT	57.2°C
h	R-GORASP2 ex7	TGCCGAGCTAATGGAGAGTC	57.2°C
i	F-GORASP2 ex8	ACTGTACCATTGCCGCC	58.4°C
j	R-GORASP2 ex9	GAGCTCTGGCAAACCGACC	58.4°C
k	F-GORASP2 ex4	AATCCAATTCCCCAGCG	57.7°C
l	R-GORASP2 ex6	GGGGGTACCAGTCATTTGTCC	57.7°C
m	F-GORASP2 ex1	CAAGTGCCACGTCCCAAGAG	58.5°C
n	R-GORASP2 ex4	CTGTGAGGTCTCAATCCTGCC	58.5°C
o	F-GORASP2 ex6	CGAATACCTACACGCCCCTT	57.2°C
p	R-GORASP2 ex10	ATGCCAGGTAAGTTTCGGGG	57.2°C
	F-GAPDH ex3–4	TGTCGTGGAGTCTACTGGTG	53.1°C
	R-GAPDH ex3–4	ACACCCATCACAAACATGG	53.1°C

List of primers used in this study. F, Forward; R, Reverse; int, intron; ex, exon.