Fly genotypes
| Figure . | Genotypes . |
|---|---|
| Fig. 1, A–D′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 1, E–H′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 1 M | c306-Gal4, UAS-cactin-RNAi/UAS-mCherry-CAAX, Gal80ts |
| Fig. 1 N | c306-Gal4, UAS-cactin-RNAi/UAS-cactin, Gal80ts |
| Fig. 1 O | c306-Gal4, UAS-cactin-RNAi/UAS-cactin-RFP, Gal80ts |
| Fig. S1 A | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. S1 B | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi (v32718GD), Gal80ts |
| Fig. S1 C | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi (v32719GD), Gal80ts |
| Fig. S1 D | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi (v106976KK), Gal80ts |
| Fig. S1, E and E′ | HSflp; AY-Gal4, UAS-GFP/UAS-cactin-RFP, UAS-w-RNAi |
| Fig. S1, F and F′ | HSflp; AY-Gal4, UAS-GFP/UAS-cactin-RFP, UAS-cactin-RNAi |
| Fig. S2, A and A′ | c306-Gal4, 10X STAT-GFP/+, Gal80ts |
| Fig. S2, B and B′ | c306-Gal4, 10X STAT-GFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S2, D and D′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. S2, E and E′ | c306-Gal4, UAS-LifeActGFP/UAS-cactus, Gal80ts |
| Fig. S2, F and F′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S2, H–I′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. S2, J and J′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S2, K and K′ | c306-Gal4, UAS-LifeActGFP/UAS-dorsal-RNAi, Gal80ts |
| Fig. 2, A–A‴ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 2, C–C‴ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 2 E | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 2 F | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 3, A–B‴ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 3, C–D‴ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 3, E, E′, G, and G′ | HSflp; AY-Gal4, UAS-GFP, UAS-w-RNAi |
| Fig. 3, F, F′, H, and H′ | HSflp; AY-Gal4, UAS-GFP, UAS-cactin-RNAi |
| Fig. 3, J–J‴ | HSflp; AY-Gal4, UAS-GFP, UAS-w-RNAi |
| Fig. 3, K–K‴ | HSflp; AY-Gal4, UAS-GFP, UAS-cactin-RNAi |
| Fig. 3, M–M″, Q, and Q′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 3, O–O″, R, and R′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S3, A–A″ | HSflp; AY-Gal4, UAS-GFP, UAS-w-RNAi |
| Fig. S3, B–B″ | HSflp; AY-Gal4, UAS-GFP, UAS-cactin-RNAi |
| Fig. S3, D–D″ | c306-Gal4, Gal80ts |
| Fig. S3, E–E″ | c306-Gal4, UAS-cactin-RNAi, Gal80ts |
| Fig. S3, F and H–H″ | c306-Gal4, sqh-mCherry/+, Gal80ts |
| Fig. S3, G and I–I″ | c306-Gal4, sqh-mCherry/UAS-cactin-RNAi, Gal80ts |
| Fig. 4, A and A′ | c306-Gal4, UAS-cactin-RNAi, Gal80ts |
| Fig. 4, B and B′ | c306-Gal4, UAS-cactin-RNAi, UAS-YFP-Rab5/Gal80ts |
| Fig. 4, C and C′ | c306-Gal4, UAS-cactin-RNAi, UAS-YFP-Rab7/Gal80ts |
| Fig. 4, D and D′ | c306-Gal4, UAS-cactin-RNAi, UAS-YFP-Rab11/Gal80ts |
| Fig. 4, E and E′ | c306-Gal4, UAS-cactin-RNAi/+, Gal80ts |
| Fig. 4, F and F′ | c306-Gal4, UAS-cactin-RNAi/aPKC-, Gal80ts |
| Fig. 4, H and H′ | c306-Gal4, EYFP-aPKC/+, UAS-GrabFP-Apical |
| Fig. 4, I and I′ | c306-Gal4, EYFP-aPKC/+, UAS-GrabFP-Basal |
| Fig. 4, J and J′ | c306-Gal4, UAS-cactin-RNAi/+, UAS-GrabFP-Basal |
| Fig. 4, K and K′ | c306-Gal4, UAS-cactin-RNAi/EYFP-aPKC, UAS-GrabFP-Basal |
| Fig. 4, L and L′ | c306-Gal4, UAS-cactin-RNAi/+, UAS-GrabFP-Apical |
| Fig. 4, M and M′ | c306-Gal4, EYFP-aPKC/+, UAS-GrabFP-Apical |
| Fig. S4, A–B′ | c306-Gal4, UAS-YFP-Rab5S43N/+, Gal80ts |
| Fig. S4, C–D′ | c306-Gal4, UAS-YFP-Rab11S25N/+, Gal80ts |
| Fig. S4, E–F′ | c306-Gal4, UAS-YFP-Rab5S43N/UAS-cactin-RNAi, Gal80ts |
| Fig. S4, G–H′ | c306-Gal4, UAS-YFP-Rab11S25N/UAS-cactin-RNAi, Gal80ts |
| Fig. S4 I | c306-Gal4, UAS-Rac-FRET/+ |
| Fig. S4 J | c306-Gal4, UAS-cactin-RNAi/+, UAS-Rac-FRET/+ |
| Fig. 5, A and A′ | c306-Gal4, Gal80ts |
| Fig. 5, B and B′ | c306-Gal4, UAS-cactin-RNAi/+, Gal80ts |
| Fig. 5, C and C′ | c306-Gal4, UAS-tsu-RNAi/+, Gal80ts |
| Fig. 5, D and D′ | c306-Gal4, UAS-SmB-RNAi/+, Gal80ts |
| Fig. 5, E and E′ | c306-Gal4, UAS-SmD3-RNAi/+, Gal80ts |
| Fig. 5, F and F′ | c306-Gal4, UAS-noi-RNAi/+, Gal80ts |
| Fig. S5, A–A″ | c306-Gal4, UAS-SmD3-RNAi/+, Gal80ts |
| Fig. S5, B–B″ | c306-Gal4, UAS-SmB-RNAi/+, Gal80ts |
| Fig. 6 E | c306-Gal4, UAS-cactin-RNAi/+, Gal80ts |
| c306-Gal4, UAS-cactin-RNAi/UAS-gkt-RNAi, Gal80ts | |
| c306-Gal4, UAS-cactin-RNAi/Sec23-, Gal80ts | |
| c306-Gal4, UAS-cactin-RNAi/Sec24CD-, Gal80ts | |
| c306-Gal4, UAS-cactin-RNAi/tws-, Gal80ts |
| Figure . | Genotypes . |
|---|---|
| Fig. 1, A–D′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 1, E–H′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 1 M | c306-Gal4, UAS-cactin-RNAi/UAS-mCherry-CAAX, Gal80ts |
| Fig. 1 N | c306-Gal4, UAS-cactin-RNAi/UAS-cactin, Gal80ts |
| Fig. 1 O | c306-Gal4, UAS-cactin-RNAi/UAS-cactin-RFP, Gal80ts |
| Fig. S1 A | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. S1 B | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi (v32718GD), Gal80ts |
| Fig. S1 C | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi (v32719GD), Gal80ts |
| Fig. S1 D | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi (v106976KK), Gal80ts |
| Fig. S1, E and E′ | HSflp; AY-Gal4, UAS-GFP/UAS-cactin-RFP, UAS-w-RNAi |
| Fig. S1, F and F′ | HSflp; AY-Gal4, UAS-GFP/UAS-cactin-RFP, UAS-cactin-RNAi |
| Fig. S2, A and A′ | c306-Gal4, 10X STAT-GFP/+, Gal80ts |
| Fig. S2, B and B′ | c306-Gal4, 10X STAT-GFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S2, D and D′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. S2, E and E′ | c306-Gal4, UAS-LifeActGFP/UAS-cactus, Gal80ts |
| Fig. S2, F and F′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S2, H–I′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. S2, J and J′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S2, K and K′ | c306-Gal4, UAS-LifeActGFP/UAS-dorsal-RNAi, Gal80ts |
| Fig. 2, A–A‴ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 2, C–C‴ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 2 E | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 2 F | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 3, A–B‴ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 3, C–D‴ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. 3, E, E′, G, and G′ | HSflp; AY-Gal4, UAS-GFP, UAS-w-RNAi |
| Fig. 3, F, F′, H, and H′ | HSflp; AY-Gal4, UAS-GFP, UAS-cactin-RNAi |
| Fig. 3, J–J‴ | HSflp; AY-Gal4, UAS-GFP, UAS-w-RNAi |
| Fig. 3, K–K‴ | HSflp; AY-Gal4, UAS-GFP, UAS-cactin-RNAi |
| Fig. 3, M–M″, Q, and Q′ | c306-Gal4, UAS-LifeActGFP/+, Gal80ts |
| Fig. 3, O–O″, R, and R′ | c306-Gal4, UAS-LifeActGFP/UAS-cactin-RNAi, Gal80ts |
| Fig. S3, A–A″ | HSflp; AY-Gal4, UAS-GFP, UAS-w-RNAi |
| Fig. S3, B–B″ | HSflp; AY-Gal4, UAS-GFP, UAS-cactin-RNAi |
| Fig. S3, D–D″ | c306-Gal4, Gal80ts |
| Fig. S3, E–E″ | c306-Gal4, UAS-cactin-RNAi, Gal80ts |
| Fig. S3, F and H–H″ | c306-Gal4, sqh-mCherry/+, Gal80ts |
| Fig. S3, G and I–I″ | c306-Gal4, sqh-mCherry/UAS-cactin-RNAi, Gal80ts |
| Fig. 4, A and A′ | c306-Gal4, UAS-cactin-RNAi, Gal80ts |
| Fig. 4, B and B′ | c306-Gal4, UAS-cactin-RNAi, UAS-YFP-Rab5/Gal80ts |
| Fig. 4, C and C′ | c306-Gal4, UAS-cactin-RNAi, UAS-YFP-Rab7/Gal80ts |
| Fig. 4, D and D′ | c306-Gal4, UAS-cactin-RNAi, UAS-YFP-Rab11/Gal80ts |
| Fig. 4, E and E′ | c306-Gal4, UAS-cactin-RNAi/+, Gal80ts |
| Fig. 4, F and F′ | c306-Gal4, UAS-cactin-RNAi/aPKC-, Gal80ts |
| Fig. 4, H and H′ | c306-Gal4, EYFP-aPKC/+, UAS-GrabFP-Apical |
| Fig. 4, I and I′ | c306-Gal4, EYFP-aPKC/+, UAS-GrabFP-Basal |
| Fig. 4, J and J′ | c306-Gal4, UAS-cactin-RNAi/+, UAS-GrabFP-Basal |
| Fig. 4, K and K′ | c306-Gal4, UAS-cactin-RNAi/EYFP-aPKC, UAS-GrabFP-Basal |
| Fig. 4, L and L′ | c306-Gal4, UAS-cactin-RNAi/+, UAS-GrabFP-Apical |
| Fig. 4, M and M′ | c306-Gal4, EYFP-aPKC/+, UAS-GrabFP-Apical |
| Fig. S4, A–B′ | c306-Gal4, UAS-YFP-Rab5S43N/+, Gal80ts |
| Fig. S4, C–D′ | c306-Gal4, UAS-YFP-Rab11S25N/+, Gal80ts |
| Fig. S4, E–F′ | c306-Gal4, UAS-YFP-Rab5S43N/UAS-cactin-RNAi, Gal80ts |
| Fig. S4, G–H′ | c306-Gal4, UAS-YFP-Rab11S25N/UAS-cactin-RNAi, Gal80ts |
| Fig. S4 I | c306-Gal4, UAS-Rac-FRET/+ |
| Fig. S4 J | c306-Gal4, UAS-cactin-RNAi/+, UAS-Rac-FRET/+ |
| Fig. 5, A and A′ | c306-Gal4, Gal80ts |
| Fig. 5, B and B′ | c306-Gal4, UAS-cactin-RNAi/+, Gal80ts |
| Fig. 5, C and C′ | c306-Gal4, UAS-tsu-RNAi/+, Gal80ts |
| Fig. 5, D and D′ | c306-Gal4, UAS-SmB-RNAi/+, Gal80ts |
| Fig. 5, E and E′ | c306-Gal4, UAS-SmD3-RNAi/+, Gal80ts |
| Fig. 5, F and F′ | c306-Gal4, UAS-noi-RNAi/+, Gal80ts |
| Fig. S5, A–A″ | c306-Gal4, UAS-SmD3-RNAi/+, Gal80ts |
| Fig. S5, B–B″ | c306-Gal4, UAS-SmB-RNAi/+, Gal80ts |
| Fig. 6 E | c306-Gal4, UAS-cactin-RNAi/+, Gal80ts |
| c306-Gal4, UAS-cactin-RNAi/UAS-gkt-RNAi, Gal80ts | |
| c306-Gal4, UAS-cactin-RNAi/Sec23-, Gal80ts | |
| c306-Gal4, UAS-cactin-RNAi/Sec24CD-, Gal80ts | |
| c306-Gal4, UAS-cactin-RNAi/tws-, Gal80ts |