Table 2.
Data collection and refinement statistics for PkKKT2551–679
Data collectionPkKKT2a551–679
BeamlineDiamond I24Diamond I03
Wavelength (Å) 0.96861 1.28272 
Space group (Z) P64 (6) P64 (6) 
Unit cell (cell edges in Å, cell angles in degrees) 113.84, 113.84, 46.01, 90, 90, 120 114.33, 114.33, 46.32 90, 90, 120 
Resolution range (Å) 56.92–2.87 (2.92–2.87) 99.01–3.80 (3.86–3.80) 
Total no. of reflections 153,076 (6844) 68,026 (3882) 
No. of unique reflections 7,986 (357) 3,535 (189) 
Completeness (%) 99.73 (91.77) 100.00 (100.00) 
Rmerge 0.10 (1.04) 0.31 (6.13) 
Rpim 0.024 (0.240) 0.073 (1.38) 
CC1/2 0.99 (0.45) 0.99 (0.4) 
[I/σ(I)] 16.45 (2.14) 12 (1.7) 
Multiplicity 19.17 (19.17) 19.2 (20.5) 
Anomalous completeness (%) 99.6 (91.2) 100 (100) 
Anomalous multiplicity 10 (9.9) 10.2 (10.6) 
Overall B factor from Wilson plot (Å2105.95 136.7 
Refinement   
Resolution (Å) 49–2.87 (3–2.87)  
No. of reflections working set 7,408 (417)  
No. of reflections test set 377 (16)  
Final Rwork (%) 25.2 (36.6)  
Final Rfree (%) 27.5 (51.2)  
No. of protein atoms 832  
No. of Zn atoms  
Average B factor (Å2) protein atoms 113.4  
Average B factor (Å2) Zn atoms 100.6  
RMSD bond lengths (Å) 0.009  
RMSD bond angles (°) 1.11  
Ramachandran plot   
Most favored (%) 90.9  
Allowed (%) 9.1  
Disallowed  
Data collectionPkKKT2a551–679
BeamlineDiamond I24Diamond I03
Wavelength (Å) 0.96861 1.28272 
Space group (Z) P64 (6) P64 (6) 
Unit cell (cell edges in Å, cell angles in degrees) 113.84, 113.84, 46.01, 90, 90, 120 114.33, 114.33, 46.32 90, 90, 120 
Resolution range (Å) 56.92–2.87 (2.92–2.87) 99.01–3.80 (3.86–3.80) 
Total no. of reflections 153,076 (6844) 68,026 (3882) 
No. of unique reflections 7,986 (357) 3,535 (189) 
Completeness (%) 99.73 (91.77) 100.00 (100.00) 
Rmerge 0.10 (1.04) 0.31 (6.13) 
Rpim 0.024 (0.240) 0.073 (1.38) 
CC1/2 0.99 (0.45) 0.99 (0.4) 
[I/σ(I)] 16.45 (2.14) 12 (1.7) 
Multiplicity 19.17 (19.17) 19.2 (20.5) 
Anomalous completeness (%) 99.6 (91.2) 100 (100) 
Anomalous multiplicity 10 (9.9) 10.2 (10.6) 
Overall B factor from Wilson plot (Å2105.95 136.7 
Refinement   
Resolution (Å) 49–2.87 (3–2.87)  
No. of reflections working set 7,408 (417)  
No. of reflections test set 377 (16)  
Final Rwork (%) 25.2 (36.6)  
Final Rfree (%) 27.5 (51.2)  
No. of protein atoms 832  
No. of Zn atoms  
Average B factor (Å2) protein atoms 113.4  
Average B factor (Å2) Zn atoms 100.6  
RMSD bond lengths (Å) 0.009  
RMSD bond angles (°) 1.11  
Ramachandran plot   
Most favored (%) 90.9  
Allowed (%) 9.1  
Disallowed  

Parentheses indicate the values relative to the highest-resolution shell.

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