| Phospholipid . | Rhodopsin . | ABCA4 . | PRPH2/ROM1 . | ROS diskb . | ROS PMb . |
|---|---|---|---|---|---|
| Copurifying in SMALPs | Isolated in ricin/gold separation | ||||
| PC | 39.6 ± 3.8 | 60.9 ± 15.6 | 60.1 ± 11.8 | 45.3 ± 3.2 | 65.1 ± 3.8 |
| PE | 54.0 ± 2.7 | 34.7 ± 17.3 | 37.0 ± 11.4 | 41.6 ± 2.6 | 10.6 ± 2.8 |
| PG | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.03 | - | - |
| PI | 1.1 ± 0.6 | 0.6 ± 0.7 | 0.2 ± 0.1 | 2.5 ± 0.8 | <1.0 |
| PS | 5.1 ± 3.4 | 3.7 ± 2.9 | 2.6 ± 0.6 | 13.7 ± 2.1 | 24.1 ± 2.8 |
| Phospholipid . | Rhodopsin . | ABCA4 . | PRPH2/ROM1 . | ROS diskb . | ROS PMb . |
|---|---|---|---|---|---|
| Copurifying in SMALPs | Isolated in ricin/gold separation | ||||
| PC | 39.6 ± 3.8 | 60.9 ± 15.6 | 60.1 ± 11.8 | 45.3 ± 3.2 | 65.1 ± 3.8 |
| PE | 54.0 ± 2.7 | 34.7 ± 17.3 | 37.0 ± 11.4 | 41.6 ± 2.6 | 10.6 ± 2.8 |
| PG | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.03 | - | - |
| PI | 1.1 ± 0.6 | 0.6 ± 0.7 | 0.2 ± 0.1 | 2.5 ± 0.8 | <1.0 |
| PS | 5.1 ± 3.4 | 3.7 ± 2.9 | 2.6 ± 0.6 | 13.7 ± 2.1 | 24.1 ± 2.8 |
PI, phosphatidylinositol.
Total values for all phospholipids detected in positive mode of LC/MS were used to estimate the relative phospholipid composition in each SMALP-extracted membrane region. Each value is presented as a mean percentage ± SD.
Comparison values from prior ROS disk and PM isolations are taken from Boesze-Battaglia and Albert (1992). Values for PG not included in Boesze-Battaglia and Albert (1992) are noted with “-,” and “< 1.0” refers to a value not reported for being <1%.