Table 1.

Primers used for plasmid construction

PrimersSequence (5′ to 3′)
PLLS229 TGA​TTG​CGT​CCT​TTA​TGT​TGT​TTT​AGA​GCT​AGA​AAT​AGC 
PLLS230 AAC​ATA​AAG​GAC​GCA​ATC​ACA​AGA​CAT​CTC​GCA​ATA​GGA​G 
PLLS1333 ATG​GGC​TTG​GAA​CAC​CCA​TGT​TTT​AGA​GCT​AGA​AAT​AGC 
PLLS1334 ATG​GGT​GTT​CCA​AGC​CCA​TCA​AGA​CAT​CTC​GCA​ATA​GGA​G 
PLLS231 GTT​CCA​AGC​CCA​TAT​GGA​TCC​GTT​CCA​CCT​CCG 
PLLS232 TGT​AGT​TTG​GAA​TTG​AGG​TAT​CAC​TAA​TCA​ATA​CAA​TTT​ATT​TGT​ATA​GTT​CAT​CCA​TGC​CAT​G 
PLLS498 GTT​CCA​AGC​CCA​TAT​GGA​TCC​GTT​CCA​CCT​CCG​ACA​AAG​CAC​AAG​GGC​AGA​AAC​CAC​ATG​GTG​TCT​AAG​GGC​GAA​GAG​CTG 
PLLS499 TGT​AGT​TTG​GAA​TTG​AGG​TAT​CAC​TAA​TCA​ATA​CAA​T TTA​CTT​GTA​CAG​CTC​GTC​CAT​GCC​ATT 
PLLS1327 TGG​CTA​GCG​TCG​ACG​GTA​CCG​CTG​TTT​CTG​CAG​ACA​CAT​CTG 
PLLS1328 AAT​AAG​CTC​TGA​CAT​GGT​ACC​ATG​ATT​GCG​TCC​TTT​ATG​TTT​G 
PLLS1329 CAC​AAG​CTT​AAT​TAG​GAG​CTC​ATT​GTA​TTG​ATT​AGT​GAT​ACC 
PLLS1330 GAC​AGC​GGC​CGA​TGC​GGA​GCT​CCC​TCT​CGC​TGT​TCA​CAA​GTT​CC 
PLLS1337 CGG​ACC​GAT​CGG​TGT​TCC​AAG​CCC​ATA​TGG​ATC​CGT​T 
PLLS1338 GAA​CAC​CGA​TCG​GTC​CGA​AAT​GGC​CTG​GTG​GTG​GCA​G 
PLLS764 GCG​GAT​CCA​AAG​TAT​TGT​CCG​CAA​GGC​ACA​TC 
PTCH5 GCG​GTA​CCG​TCG​AAG​AAA​GAA​CGG​TTT 
PJYH251 CTC​TAG​AAA​GTA​TAG​GAA​CTT​CGC​ATG​CCA​TGA​TTA​CGC​CAA​GCT​TGC​ATG​C 
PJYH252 CAA​TAG​AGG​ACC​GCT​ACA​CAC​TAG​TCG​CGA​GAC​GAA​AGG​GCC​C 
PLLS765 GCG​GAT​CCG​TAA​ATT​ATT​TTA​TTA​GTA​AAA​AGG 
PLLS682 GCG​CTA​GCA​TGG​TTG​GAG​AGA​GGT​ATC​GTA​G 
PLLS683 GCG​GTA​CCC​TCC​GGC​TCG​TGT​GGA​AGA​GC 
PLLS821 GCC​ATA​TGA​TGG​GCA​AGC​AGG​GAG​AAG​AAG​C 
PLLS822 GCG​GAT​CCA​TTT​ATA​CAG​AAC​AAA​GTG​CTG​CAA​CAA​G 
PTCH144 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGC​TGG​CGG​ACC​AAC​GTG​TAC 
PTCH145 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AAT​GAT​TGC​GTC​CTT​TAT​G 
PTCH150 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGG​GGT​CGT​TGT​CGC​GCT​CG 
PTCH151 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AAG​CTG​CTT​CAG​CCT​CCT​GAA​G 
PTCH152 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGG​GAA​TCA​GCG​ACA​ACG​AC 
PTCH153 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AGT​CGA​AGA​AAG​AAC​GGT​TT 
PTCH156 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGG​CCG​CAG​AAT​CTT​CG 
PTCH157 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AAT​CCT​CGA​GGT​TTC​TGA​A 
PLLS3613 TGA​TCT​CAG​AGG​AGG​ACC​TGC​ATA​TGT​CAC​TTG​AGC​AAC​AAG​TAA​AAT​A 
PLLS3614 TGC​GGC​CGC​TGC​AGG​TCG​ACG​GAT​CCT​TAA​TGA​TTG​CGT​CCT​TTA​TGT​TTG 
PLLS3615 TGA​TCT​CAG​AGG​AGG​ACC​TGC​ATA​TGA​TGA​GTA​GCT​CCC​ACT​CTC​GGG​C 
PLLS3616 TGC​GGC​CGC​TGC​AGG​TCG​ACG​GAT​CCt​taG​AAC​TGG​GAC​AGG​TAG​GTC​ATG 
PLLS1819 CTG​TTC​CAG​GGG​CCC​CTG​GGA​TCC​ATG​GGC​AAG​CAG​GGA​GAA​GAA​G 
PLLS1822 GAT​CGT​CAG​TCA​GTC​ACG​ATG​CGG​CCG​CTT​ATA​CAG​AAC​AAA​GTG​CTG​CAA​CAA​G 
PLLS1485 ATG​CCA​TAT​GAT​GGG​AAT​CAG​CGA​CAA​CGA​CG 
PLLS1457 GCC​TCG​AGG​TCG​AAG​AAA​GAA​CGG​TTT​GG 
PLLS1765 GCA​TGC​ATA​TGA​TGG​CCG​CAG​AAT​CTT​CGT​ACG 
PLLS1766 GCG​CGG​CCG​CAT​CCT​CGA​GGT​TTC​TGA​AAG​CG 
PrimersSequence (5′ to 3′)
PLLS229 TGA​TTG​CGT​CCT​TTA​TGT​TGT​TTT​AGA​GCT​AGA​AAT​AGC 
PLLS230 AAC​ATA​AAG​GAC​GCA​ATC​ACA​AGA​CAT​CTC​GCA​ATA​GGA​G 
PLLS1333 ATG​GGC​TTG​GAA​CAC​CCA​TGT​TTT​AGA​GCT​AGA​AAT​AGC 
PLLS1334 ATG​GGT​GTT​CCA​AGC​CCA​TCA​AGA​CAT​CTC​GCA​ATA​GGA​G 
PLLS231 GTT​CCA​AGC​CCA​TAT​GGA​TCC​GTT​CCA​CCT​CCG 
PLLS232 TGT​AGT​TTG​GAA​TTG​AGG​TAT​CAC​TAA​TCA​ATA​CAA​TTT​ATT​TGT​ATA​GTT​CAT​CCA​TGC​CAT​G 
PLLS498 GTT​CCA​AGC​CCA​TAT​GGA​TCC​GTT​CCA​CCT​CCG​ACA​AAG​CAC​AAG​GGC​AGA​AAC​CAC​ATG​GTG​TCT​AAG​GGC​GAA​GAG​CTG 
PLLS499 TGT​AGT​TTG​GAA​TTG​AGG​TAT​CAC​TAA​TCA​ATA​CAA​T TTA​CTT​GTA​CAG​CTC​GTC​CAT​GCC​ATT 
PLLS1327 TGG​CTA​GCG​TCG​ACG​GTA​CCG​CTG​TTT​CTG​CAG​ACA​CAT​CTG 
PLLS1328 AAT​AAG​CTC​TGA​CAT​GGT​ACC​ATG​ATT​GCG​TCC​TTT​ATG​TTT​G 
PLLS1329 CAC​AAG​CTT​AAT​TAG​GAG​CTC​ATT​GTA​TTG​ATT​AGT​GAT​ACC 
PLLS1330 GAC​AGC​GGC​CGA​TGC​GGA​GCT​CCC​TCT​CGC​TGT​TCA​CAA​GTT​CC 
PLLS1337 CGG​ACC​GAT​CGG​TGT​TCC​AAG​CCC​ATA​TGG​ATC​CGT​T 
PLLS1338 GAA​CAC​CGA​TCG​GTC​CGA​AAT​GGC​CTG​GTG​GTG​GCA​G 
PLLS764 GCG​GAT​CCA​AAG​TAT​TGT​CCG​CAA​GGC​ACA​TC 
PTCH5 GCG​GTA​CCG​TCG​AAG​AAA​GAA​CGG​TTT 
PJYH251 CTC​TAG​AAA​GTA​TAG​GAA​CTT​CGC​ATG​CCA​TGA​TTA​CGC​CAA​GCT​TGC​ATG​C 
PJYH252 CAA​TAG​AGG​ACC​GCT​ACA​CAC​TAG​TCG​CGA​GAC​GAA​AGG​GCC​C 
PLLS765 GCG​GAT​CCG​TAA​ATT​ATT​TTA​TTA​GTA​AAA​AGG 
PLLS682 GCG​CTA​GCA​TGG​TTG​GAG​AGA​GGT​ATC​GTA​G 
PLLS683 GCG​GTA​CCC​TCC​GGC​TCG​TGT​GGA​AGA​GC 
PLLS821 GCC​ATA​TGA​TGG​GCA​AGC​AGG​GAG​AAG​AAG​C 
PLLS822 GCG​GAT​CCA​TTT​ATA​CAG​AAC​AAA​GTG​CTG​CAA​CAA​G 
PTCH144 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGC​TGG​CGG​ACC​AAC​GTG​TAC 
PTCH145 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AAT​GAT​TGC​GTC​CTT​TAT​G 
PTCH150 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGG​GGT​CGT​TGT​CGC​GCT​CG 
PTCH151 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AAG​CTG​CTT​CAG​CCT​CCT​GAA​G 
PTCH152 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGG​GAA​TCA​GCG​ACA​ACG​AC 
PTCH153 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AGT​CGA​AGA​AAG​AAC​GGT​TT 
PTCH156 TAC​CAG​ATT​ACG​CTC​ATA​TGA​TGG​CCG​CAG​AAT​CTT​CG 
PTCH157 GCT​CGA​GCT​CGA​TGG​ATC​CTT​AAT​CCT​CGA​GGT​TTC​TGA​A 
PLLS3613 TGA​TCT​CAG​AGG​AGG​ACC​TGC​ATA​TGT​CAC​TTG​AGC​AAC​AAG​TAA​AAT​A 
PLLS3614 TGC​GGC​CGC​TGC​AGG​TCG​ACG​GAT​CCT​TAA​TGA​TTG​CGT​CCT​TTA​TGT​TTG 
PLLS3615 TGA​TCT​CAG​AGG​AGG​ACC​TGC​ATA​TGA​TGA​GTA​GCT​CCC​ACT​CTC​GGG​C 
PLLS3616 TGC​GGC​CGC​TGC​AGG​TCG​ACG​GAT​CCt​taG​AAC​TGG​GAC​AGG​TAG​GTC​ATG 
PLLS1819 CTG​TTC​CAG​GGG​CCC​CTG​GGA​TCC​ATG​GGC​AAG​CAG​GGA​GAA​GAA​G 
PLLS1822 GAT​CGT​CAG​TCA​GTC​ACG​ATG​CGG​CCG​CTT​ATA​CAG​AAC​AAA​GTG​CTG​CAA​CAA​G 
PLLS1485 ATG​CCA​TAT​GAT​GGG​AAT​CAG​CGA​CAA​CGA​CG 
PLLS1457 GCC​TCG​AGG​TCG​AAG​AAA​GAA​CGG​TTT​GG 
PLLS1765 GCA​TGC​ATA​TGA​TGG​CCG​CAG​AAT​CTT​CGT​ACG 
PLLS1766 GCG​CGG​CCG​CAT​CCT​CGA​GGT​TTC​TGA​AAG​CG 
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