| D.Mel-2 cultured cells . | ||||
|---|---|---|---|---|
| Proteins . | pAct5C-Rcd4-GFP . | pAct5C-Rcd4-GFP (+OA) . | ||
| Score . | Peptides . | Score . | Peptides . | |
| Rcd4 | 1001 | 30 | 1123 | 32 |
| Ana3 | 5316 | 121 | 5212 | 110 |
| pAct5C-GFP-Ana3 | pAct5C-GFP-Ana3 (+OA) | |||
| Ana3 | 10438 | 332 | 13029 | 426 |
| Rcd4 | 825 | 23 | 1042 | 32 |
| D.Mel-2 cultured cells . | ||||
|---|---|---|---|---|
| Proteins . | pAct5C-Rcd4-GFP . | pAct5C-Rcd4-GFP (+OA) . | ||
| Score . | Peptides . | Score . | Peptides . | |
| Rcd4 | 1001 | 30 | 1123 | 32 |
| Ana3 | 5316 | 121 | 5212 | 110 |
| pAct5C-GFP-Ana3 | pAct5C-GFP-Ana3 (+OA) | |||
| Ana3 | 10438 | 332 | 13029 | 426 |
| Rcd4 | 825 | 23 | 1042 | 32 |
Top: Copurification of Ana3 with Rcd4-GFP (bait) from cultured D.Mel-2 cells expressing Rcd4-GFP under the control of a constitutive actin-5c promoter. Bottom: Copurification of Rcd4 with GFP-Ana3 (bait) from cultured D.Mel-2 cells expressing GFP-Ana3 under the control of a constitutive actin-5 promoter. Extracts were made in isotonic buffer and with okadaic acid (OA) as indicated. Scores (mascot) and numbers of peptides detected by mass spectrometry are indicated.