Top: Copurification of Ana3 with Rcd4-GFP (bait) from cultured D.Mel-2 cells expressing Rcd4-GFP under the control of a constitutive actin-5c promoter. Bottom: Copurification of Rcd4 with GFP-Ana3 (bait) from cultured D.Mel-2 cells expressing GFP-Ana3 under the control of a constitutive actin-5 promoter. Extracts were made in isotonic buffer and with okadaic acid (OA) as indicated. Scores (mascot) and numbers of peptides detected by mass spectrometry are indicated.