Table 2.
CRISPR screens identify host dependency factors of viruses
RNA/DNA virusFamily, genusVirusHost factor genesHost processesReferences
RNA Flaviviridae, Flavivirus West Nile EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, HRD1, STT3A, SEC63, SPCS1, SPC3 ERAD, endoplasmic reticulum-associated signal peptidase complex (SPCS) (Ma et al., 2015); (Zhang et al., 2016) 
 Flaviviridae, Flavivirus Dengue STT3A, STT3B, OSTC, EMC2, EMC4, EMC3, SSR1, SSR2, SSR3, SEC61A1, OST4, MAGT1 ERAD, SPCS, OST (Marceau et al., 2016); (Lin et al., 2017) 
 Flaviviridae, Flavivirus Yellow fever IFI6, IFNAR1, IFNAR2, IRF9, TYK2, JAK1, STAT2, PCBP1, ECD, SNRPF, PCF11, SNRPD1, HSPA5 IFN-stimulated genes (ISG)/IFN pathway, RNA processing (Richardson et al., 2018) 
 Flaviviridae, Flavivirus Zika AXL, EMC1, EMC2, EMC3, SSR3, RABGEF1, MMGT1 Viral entry, ERAD, SPCS, endocytosis (Savidis et al., 2016); (Li et al., 2019) 
 Flaviviridae, Hepacivirus Hepatitis C CD81, CLDN1, OCLN, MIR122, PPIA, RFK, FLAD ELAVL1, SSRD Viral entry, RNA-binding proteins/mRNA stabilization, FAD metabolism, peptidyl-prolyl isomerase (Marceau et al., 2016) 
 Filoviridae, Ebolavirus Zaire Ebola NPC1, SPNS1, SLC30AI, VPS16, VPS18, VPS33A, KLHDC3, STARD13, GNPTAB Viral entry, lysosomal transport, multisubunit tethering complexes (MTCs) in the endolysosomal pathway (HOPS complex) (Flint et al., 2019) 
 Caliciviridae, Norovirus Murine norovirus CD300lf, CD300ld Viral entry (Orchard et al., 2016); (Haga et al., 2016) 
 Picornaviridae, Enterovirus Rhinovirus SETD3, PLA2G16, CSDE1 ISG/IFN pathway, viral entry, translation (IRES) (Diep et al., 2019) 
 Picornaviridae, Hepatovirus Hepatitis A GNE, CMAS, SLC35A1, UGCG, ST3GAL5, VPS4A, UFM1, UBA5, UFL1, UFC1, UFSP2, PAPD5, PAPD7, ZCCHC14, PTBP1, EIF4B, EIF3C, EIF3CL Sialic acid and ganglioside biosynthesis, translation initiation, IRES-mediated translation, endosomal sorting (ESCRT), Trf4/5–Air1/2–Mtr4 polyadenylation (TRAMP) complex, UFMylation, polyadenylation (Kulsuptrakul et al., 2020) 
 Orthomyxoviridae, Alphainfluenzavirus Influenza A SLC35A1, WDR7, EXOC4, VHL, TMEM38A, ATP6AP1 DPAGT1, cap methyltransferase 1 (CMTR1), SRP19, Sialic acid biosynthesis and transport, N-glycan biosynthesis, UPS, v-type-ATPase, RNA processing, protein export (Han et al., 2018); (Li et al., 2020) 
 Coronaviridae, Betacoronavirus SARS-CoV-2 ACE2, CTSL, switch/sucrose nonfermenting–related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 4 (SMARCA4), ARID1A, SMARCE1, KDM6A, DYRK1A, UBXN7, small body size/mothers against the decapentaplegic 4 (SMAD4), HMGB1-like; HIRAa, CABINa, ASF1Aa Viral entry and processing, chromatin remodeling, histone methylation, UPS, TGF-β signaling, alarmin (Wei et al., 2020) 
RNA, retrovirus Retroviridae, Lentivirus HIV CD4, CCR5, TPST2, SLC35B2, ALCAM, myc-induced nuclear antigen 53 (MINA53)a Viral entry, post-translational modification (sulfation), cell–cell adhesion, histone modification, latency (Park et al., 2017); (Huang et al., 2019) 
DNA Hepadnaviridae, Orthohepadnavirus Hepatitis B ZCCHC14, (PAPD5, PAPD7), NXT1, ENY2, DCAF7a, UBE2J1a, UBE2J2a, RNF139a Polyadenylation, nuclear export, UPS (Hyrina et al., 2019) 
 Herpesviridae, Lymphocryptovirus Epstein-Barr CD19b, CD81b, IRF2b, IRF4b, SYKb, BATFb,CFLARb, RBPJb, RelAb, RFN31b, CCND2b, CDK6 b, CDK4c, CCND3c, BCL6c Cell cycle, LMP1/LMP2a signaling, PI3K/AKT signaling, tumor suppression pathways (Ma et al., 2017) 
RNA/DNA virusFamily, genusVirusHost factor genesHost processesReferences
RNA Flaviviridae, Flavivirus West Nile EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, HRD1, STT3A, SEC63, SPCS1, SPC3 ERAD, endoplasmic reticulum-associated signal peptidase complex (SPCS) (Ma et al., 2015); (Zhang et al., 2016) 
 Flaviviridae, Flavivirus Dengue STT3A, STT3B, OSTC, EMC2, EMC4, EMC3, SSR1, SSR2, SSR3, SEC61A1, OST4, MAGT1 ERAD, SPCS, OST (Marceau et al., 2016); (Lin et al., 2017) 
 Flaviviridae, Flavivirus Yellow fever IFI6, IFNAR1, IFNAR2, IRF9, TYK2, JAK1, STAT2, PCBP1, ECD, SNRPF, PCF11, SNRPD1, HSPA5 IFN-stimulated genes (ISG)/IFN pathway, RNA processing (Richardson et al., 2018) 
 Flaviviridae, Flavivirus Zika AXL, EMC1, EMC2, EMC3, SSR3, RABGEF1, MMGT1 Viral entry, ERAD, SPCS, endocytosis (Savidis et al., 2016); (Li et al., 2019) 
 Flaviviridae, Hepacivirus Hepatitis C CD81, CLDN1, OCLN, MIR122, PPIA, RFK, FLAD ELAVL1, SSRD Viral entry, RNA-binding proteins/mRNA stabilization, FAD metabolism, peptidyl-prolyl isomerase (Marceau et al., 2016) 
 Filoviridae, Ebolavirus Zaire Ebola NPC1, SPNS1, SLC30AI, VPS16, VPS18, VPS33A, KLHDC3, STARD13, GNPTAB Viral entry, lysosomal transport, multisubunit tethering complexes (MTCs) in the endolysosomal pathway (HOPS complex) (Flint et al., 2019) 
 Caliciviridae, Norovirus Murine norovirus CD300lf, CD300ld Viral entry (Orchard et al., 2016); (Haga et al., 2016) 
 Picornaviridae, Enterovirus Rhinovirus SETD3, PLA2G16, CSDE1 ISG/IFN pathway, viral entry, translation (IRES) (Diep et al., 2019) 
 Picornaviridae, Hepatovirus Hepatitis A GNE, CMAS, SLC35A1, UGCG, ST3GAL5, VPS4A, UFM1, UBA5, UFL1, UFC1, UFSP2, PAPD5, PAPD7, ZCCHC14, PTBP1, EIF4B, EIF3C, EIF3CL Sialic acid and ganglioside biosynthesis, translation initiation, IRES-mediated translation, endosomal sorting (ESCRT), Trf4/5–Air1/2–Mtr4 polyadenylation (TRAMP) complex, UFMylation, polyadenylation (Kulsuptrakul et al., 2020) 
 Orthomyxoviridae, Alphainfluenzavirus Influenza A SLC35A1, WDR7, EXOC4, VHL, TMEM38A, ATP6AP1 DPAGT1, cap methyltransferase 1 (CMTR1), SRP19, Sialic acid biosynthesis and transport, N-glycan biosynthesis, UPS, v-type-ATPase, RNA processing, protein export (Han et al., 2018); (Li et al., 2020) 
 Coronaviridae, Betacoronavirus SARS-CoV-2 ACE2, CTSL, switch/sucrose nonfermenting–related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 4 (SMARCA4), ARID1A, SMARCE1, KDM6A, DYRK1A, UBXN7, small body size/mothers against the decapentaplegic 4 (SMAD4), HMGB1-like; HIRAa, CABINa, ASF1Aa Viral entry and processing, chromatin remodeling, histone methylation, UPS, TGF-β signaling, alarmin (Wei et al., 2020) 
RNA, retrovirus Retroviridae, Lentivirus HIV CD4, CCR5, TPST2, SLC35B2, ALCAM, myc-induced nuclear antigen 53 (MINA53)a Viral entry, post-translational modification (sulfation), cell–cell adhesion, histone modification, latency (Park et al., 2017); (Huang et al., 2019) 
DNA Hepadnaviridae, Orthohepadnavirus Hepatitis B ZCCHC14, (PAPD5, PAPD7), NXT1, ENY2, DCAF7a, UBE2J1a, UBE2J2a, RNF139a Polyadenylation, nuclear export, UPS (Hyrina et al., 2019) 
 Herpesviridae, Lymphocryptovirus Epstein-Barr CD19b, CD81b, IRF2b, IRF4b, SYKb, BATFb,CFLARb, RBPJb, RelAb, RFN31b, CCND2b, CDK6 b, CDK4c, CCND3c, BCL6c Cell cycle, LMP1/LMP2a signaling, PI3K/AKT signaling, tumor suppression pathways (Ma et al., 2017) 

Genes listed are proviral unless annotated as antiviral (a) genes. Screens for Epstein-Barr virus were performed in lymphoblastoid (b) or Burkitt lymphoma (c) cell lines.

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