Table 2. Cross-reactivity of anti-POWV... | Rockefeller University Press

Table 2.

Cross-reactivity of anti-POWV mAbs against TBFVs

Competition group	mAb	Binding (flow cytometry)^a			EC50 (ng/ml)^b
Competition group	mAb	TBEV	LGTV	GGYV	TBEV	LGTV	GGYV
None	POWV-16	++	++
A	POWV-4	++	++	++
B	POWV-5
	POWV-18
	POWV-54	++	++	++		1,480	7,818
	POWV-56	++	++	++		11,477
	POWV-63	++	++	++		2,520	11,183
C	POWV-14					10,289
	POWV-15
	POWV-23
D	POWV-48
	POWV-62			++
	POWV-70			++
E	POWV-33	++			1,615
	POWV-37	++			587
	POWV-65
	POWV-71	++	++			44
	POWV-80	++	++		8,475	10,354
F	POWV-55
	POWV-60
	POWV-61

Competition group	mAb	Binding (flow cytometry)^a			EC50 (ng/ml)^b
Competition group	mAb	TBEV	LGTV	GGYV	TBEV	LGTV	GGYV
None	POWV-16	++	++
A	POWV-4	++	++	++
B	POWV-5
	POWV-18
	POWV-54	++	++	++		1,480	7,818
	POWV-56	++	++	++		11,477
	POWV-63	++	++	++		2,520	11,183
C	POWV-14					10,289
	POWV-15
	POWV-23
D	POWV-48
	POWV-62			++
	POWV-70			++
E	POWV-33	++			1,615
	POWV-37	++			587
	POWV-65
	POWV-71	++	++			44
	POWV-80	++	++		8,475	10,354
F	POWV-55
	POWV-60
	POWV-61

Cross-reactivity to other TBFVs was determined by mAb binding of C-prM-E–transfected cells by flow cytometry.

Cross-neutralization was assessed by FRNT (LGTV) or an RVP-based assay (TBEV and GGYV) in Vero cells. Geometric mean EC50 values are from two to three independent experiments.

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