Table 1. POWV mAb competition group and... | Rockefeller University Press

Table 1.

POWV mAb competition group and neutralization potency

Competition group	mAb	VH gene	Isotype	ELISA reactivity^a	K_D kinetic (nM ± SD)^b	t_1/2 (s)	EC50 (ng/ml)^c
Competition group	mAb	VH gene	Isotype	ELISA reactivity^a	K_D kinetic (nM ± SD)^b	t_1/2 (s)	POWV RVP	Lineage II SPO	Lineage II MA51240	Lineage I LB
None	POWV-16	IGHV1-39*01	IgG2c	None	N/A	N/A	10	>20,000	>20,000	>20,000
A	POWV-4	IGHV1-62-2*01	IgG2c	E	9.7 ± 1.7	285	43	3,658	368	10,856
B	POWV-5	IGHV1-50*01	IgG2c	E	2.8 ± 0.47	2,214	311	6,631	1,049	5,269
	POWV-18	IGHV1-50*01	IgG2b	E	3.2 ± 0.81	2,390	803	8,507	1,578	10,504
	POWV-54	IGHV1-26*01	IgG2c	E	1.3 ± 0.2	3,536	9	1,757	231	2,260
	POWV-63	IGHV1-26*01	IgG2c	E	3.2 ± 0.4	721	6	5,018	223	2,999
	POWV-56	IGHV1-53*01	IgG2c	E	2.0 ± 0.41	1,724	6	2,612	310	726
C	POWV-14	IGHV1-62-2*01	IgG2c	E	1.7 ± 0.98	2,740	122	4,085	340	12,248
	POWV-15	IGHV1-15*01	IgG2c	E	23 ± 1.91	171	358	10,840	660	>20,000
	POWV-23	IGHV1-67*01	IgG2c	E	4.0 ± 0.68	1,920	111	3,800	318	>20,000
D	POWV-48	IGHV1-34*01	IgG2b	E	3.6 ± 0.12	1,075	234	5,395	434	3,149
	POWV-62	IGHV1-34*01	IgG2c	E	0.6 ± 0.43	1,998	83	2,412	226	843
	POWV-70	IGHV1-34*01	IgG2c	E	1.6 ± 0.39	1,853	87	1,358	202	1,054
E	POWV-33	IGHV1-75*01	IgG2c	E, DIII	2.5 ± 0.05	1,066	95	5,307	162	7,577
	POWV-37	IGHV1-75*01	IgG2c	E, DIII	1.8 ± 0.17	837	51	3,455	127	8,411
	POWV-65	IGHV1-22*01	IgG2c	E, DIII	1.5 ± 0.17	2,840	102	7,955	110	4,854
	POWV-71	IGHV1-54*01	IgG2b	E, DIII	0.6 ± 0.26	4,151	27	14,924	339	6,789
	POWV-80	IGHV5-16*02	IgG2c	E, DIII	34.7 ± 4.47	84	104	12,060	83	17,962
F	POWV-55	IGHV14-3*01	IgG2c	E, DIII	1.2 ± 0.35	1,066	16	1,764	23	5,410
	POWV-60	IGHV14-3*01	IgG2c	E, DIII	1.9 ± 0.58	857	19	2,621	21	6,913
	POWV-61	IGHV14-3*01	IgG2c	E, DIII	2.1 ± 0.19	1,122	16	1,839	21	4,463

Competition group	mAb	VH gene	Isotype	ELISA reactivitya	K_D kinetic (nM ± SD)b	t_1/2 (s)	EC50 (ng/ml)c
Competition group	mAb	VH gene	Isotype	ELISA reactivitya	K_D kinetic (nM ± SD)b	t_1/2 (s)	POWV RVP	Lineage II SPO	Lineage II MA51240	Lineage I LB
None	POWV-16	IGHV1-39*01	IgG2c	None	N/A	N/A	10	>20,000	>20,000	>20,000
A	POWV-4	IGHV1-62-2*01	IgG2c	E	9.7 ± 1.7	285	43	3,658	368	10,856
B	POWV-5	IGHV1-50*01	IgG2c	E	2.8 ± 0.47	2,214	311	6,631	1,049	5,269
	POWV-18	IGHV1-50*01	IgG2b	E	3.2 ± 0.81	2,390	803	8,507	1,578	10,504
	POWV-54	IGHV1-26*01	IgG2c	E	1.3 ± 0.2	3,536	9	1,757	231	2,260
	POWV-63	IGHV1-26*01	IgG2c	E	3.2 ± 0.4	721	6	5,018	223	2,999
	POWV-56	IGHV1-53*01	IgG2c	E	2.0 ± 0.41	1,724	6	2,612	310	726
C	POWV-14	IGHV1-62-2*01	IgG2c	E	1.7 ± 0.98	2,740	122	4,085	340	12,248
	POWV-15	IGHV1-15*01	IgG2c	E	23 ± 1.91	171	358	10,840	660	>20,000
	POWV-23	IGHV1-67*01	IgG2c	E	4.0 ± 0.68	1,920	111	3,800	318	>20,000
D	POWV-48	IGHV1-34*01	IgG2b	E	3.6 ± 0.12	1,075	234	5,395	434	3,149
	POWV-62	IGHV1-34*01	IgG2c	E	0.6 ± 0.43	1,998	83	2,412	226	843
	POWV-70	IGHV1-34*01	IgG2c	E	1.6 ± 0.39	1,853	87	1,358	202	1,054
E	POWV-33	IGHV1-75*01	IgG2c	E, DIII	2.5 ± 0.05	1,066	95	5,307	162	7,577
	POWV-37	IGHV1-75*01	IgG2c	E, DIII	1.8 ± 0.17	837	51	3,455	127	8,411
	POWV-65	IGHV1-22*01	IgG2c	E, DIII	1.5 ± 0.17	2,840	102	7,955	110	4,854
	POWV-71	IGHV1-54*01	IgG2b	E, DIII	0.6 ± 0.26	4,151	27	14,924	339	6,789
	POWV-80	IGHV5-16*02	IgG2c	E, DIII	34.7 ± 4.47	84	104	12,060	83	17,962
F	POWV-55	IGHV14-3*01	IgG2c	E, DIII	1.2 ± 0.35	1,066	16	1,764	23	5,410
	POWV-60	IGHV14-3*01	IgG2c	E, DIII	1.9 ± 0.58	857	19	2,621	21	6,913
	POWV-61	IGHV14-3*01	IgG2c	E, DIII	2.1 ± 0.19	1,122	16	1,839	21	4,463

N/A, not available.

mAbs were assessed for binding to recombinant POWV E protein and DIII of the E protein by ELISA.

Example kinetic binding curves with fits shown in Fig. S4.

Neutralization of POWV strains was assessed by FRNT or an RVP-based assay where noted. EC50 values were determined by nonlinear regression. Geometric mean from three or four independent experiments.

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