Table 1

Primer list

Primer Sequence 
NPC2 S3 5ʹ-AGG​ATG​ATC​TGA​TCA​CTT​GCT​TAA​CCG​GAG​AAG​TCA​TCT​TCC​CAC​CAA​GGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
NPC2 S2 5ʹ-GAA​CGA​GAA​GGG​AAA​TAA​ACA​CGG​ATC​AAT​GAG​TTG​TAT​GAA​TCA​GAT​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
VPS10 S1 5ʹ-CTG​TAT​ATC​TGG​AAA​AGC​CCT​GAA​GTG​TCC​AGT​AGT​CAT​CAC​ACG​TTA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
VPS10 S2 5ʹ-GTT​TAT​GAA​AAG​TAT​ATG​GAA​TTA​TCT​ACT​CTA​TGT​AAA​GTA​ATC​TCT​CTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3ʹ 
CPY S2 5ʹ-TAA​AGC​GTG​TAT​GTG​TAG​GCA​TAC​CGT​TTT​TAT​TAT​CAG​CTA​CGA​TCG​AAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
CPY S3 5ʹ-AAA​ACG​CCT​TAA​GTA​TGG​TTA​ACG​AAT​GGA​TCC​ACG​GTG​GTT​TCT​CCT​TAC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APM3 S1 5ʹ-GAC​TTG​GGC​AAC​AAC​AGA​GGC​TGT​AAA​CCT​TAC​CAA​CCC​AAC​CAA​AAT​AGA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APM3 S2 5ʹ-CTC​ATT​ATA​TTC​TAT​TTA​GTT​TCG​CAT​GGA​ATT​TCA​AGT​ACA​CAT​ATA​ACT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3ʹ 
APM3 for 5ʹ-GAT​ATC​GAA​TTC​CTG​GAG​GGG​TAG​AAG​TCG​CTG-3ʹ 
APM3 rev 5ʹ-GGA​TCC​CCC​GGG​CTG​TGC​GGA​AGT​CTT​CCC​TAA​G-3ʹ 
pRS APM3 for 5ʹ-AGG​GAA​GAC​TTC​CGC​ACA​GCC​CGG​GGG​ATC​CAC-3ʹ 
pRS APM3 rev 5ʹ-GCG​ACT​TCT​ACC​CCT​CCA​GGA​ATT​CGA​TAT​CAA​GCT​TAT​CG-3ʹ 
APM3 D217A for 5ʹ-GCT​TTA​TGT​GGC​TTT​GTT​AGA​AAC-3ʹ 
APM3 D217A rev 5ʹ-TCA​TTA​TTC​TCA​TGT​TTG​CTA​G-3ʹ 
APM3 G469K for 5ʹ-GCT​GTA​CAA​GAA​GGC​AAA​GTA​TAA​GAC-3ʹ 
APM3 G469K rev 5ʹ-TTT​GTA​TTT​TTG​CCA​ATT​GTT​AAT​C-3ʹ 
APS3 S1 5ʹ-CTA​TAC​CAG​ATC​TGG​GTT​ACA​GAG​GCC​AGA​ACA​CAT​ATA​ACG​TTA​CAC​AAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
APS3 S2 5ʹ-CAG​ATC​TTG​ACG​AAC​GAA​AAT​AAC​CAT​CAT​CCG​ATT​AAT​TTG​TTT​GTT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
APS3 for 5ʹ-TGA​TAT​CGA​ATT​CCT​GCT​TTT​GAG​TAT​GAG​GAG​TTC​CAC-3ʹ 
APS3 rev 5ʹ-TGG​ATC​CCC​CGG​GCT​GGT​GGA​TTT​TGC​CAT​TTC​TAA​AGA​G-3ʹ 
pRS APS3 for 5ʹ-AAA​TGG​CAA​AAT​CCA​CCA​GCC​CGG​GGG​ATC​CAC-3ʹ 
pRS APS3 rev 5ʹ-TCC​TCA​TAC​TCA​AAA​GCA​GGA​ATT​CGA​TAT​CAA​GCT​TAT​CG-3ʹ 
APS3 V106D for 5ʹ-CCA​AAC​TTT​TGA​CGA​ATC​ATT​GGA​C-3ʹ 
APS3 V106D rev 5ʹ-ATC​AGA​TCT​AAT​ATG​GCC-3ʹ 
APS3 L121S for 5ʹ-TGA​ACT​TGA​TTC​GAT​TTT​TAA​CTG​G-3ʹ 
APS3 L121S rev 5ʹ-TTG​ACT​TCA​GTA​AAA​CAA​CG-3ʹ 
GGA1 S1 5ʹ-GGG​ACA​AGT​CAC​TAC​TTC​AAG​TAT​AAC​CCA​GAC​AAG​AGT​CTT​TTA​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
GGA2 S2 5ʹ-ATA​TAA​TAT​GGC​ATC​TAC​TTT​TTT​TTC​AAC​TTC​TCT​ACC​GAA​TTT​GAT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
GGA2 S1 5ʹ-AAG​AGC​AAC​ATA​ACT​CAT​AGC​TGA​ATT​GCT​AAT​CGT​GAT​ACT​GCA​TCA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
GGA2 S2 5ʹ-AAA​ATA​TAC​ATA​GAG​AAG​AGA​AAG​GAT​TGA​TAA​GAA​ACG​CCA​GAG​GAT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
VPS45 S1 5ʹ-GAC​TTG​GTT​TTG​AGT​TAA​GGC​CAT​CTT​TTA​CTG​TAT​AGA​ACA​AAG​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
VPS45 S2 5ʹ-GAT​TTA​TGC​CTC​ATA​TAT​AAA​ATA​GAA​TTT​TAG​AAT​AAG​ATA​ATC​CTT​ATT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
APL5 S1 5ʹ-GAA​GTG​GGA​AAG​GCA​GTT​GCC​ACA​ACC​AGA​AGA​GCA​TAA​CAT​ATT​GTC​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
APL5 S2 5ʹ-CGT​AAT​CGC​TGT​CAT​TGA​GAA​TTC​ACG​ACT​TCG​ATT​TTT​TTA​TCG​ATC​GAT​GAA​TTC​GAG​CTC​G-3ʹ 
ATG19 S1 5ʹ-TTG​CGG​CGG​CAC​TTG​CTT​CAG​TAA​CGC​CCA​AAG​GAG​AGT​TCT​GGT​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG19 S2 5ʹ-TGT​GTA​TGT​GAA​AAG​GTA​CTC​ATT​GCT​GTA​TAA​AAA​TAG​AGT​TTG​ACC​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
LAP3 S1 5ʹ-CGT​TTA​GAA​GCC​ACC​TTT​TAC​GAG​CAC​CAC​AAA​TTG​TTT​TAA​AAA​GAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
LAP3 S4 5ʹ-TGA​AAC​TCT​TTG​TTC​CAA​GAG​TTG​ATC​TTA​CTG​ATA​TCG​ATG​GAA​GAG​GAC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
VPH1 S2 5ʹ-TAT​TTA​ATG​AAG​TAC​TTA​AAT​GTT​TCG​CTT​TTT​TTA​AAA​GTC​CTC​AAA​ATA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
VPH1 S3 5ʹ-GTA​TAA​AGA​CAT​GGA​AGT​CGC​TGT​TGC​TAG​TGC​AAG​CTC​TTC​CGC​TTC​AAG​CCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
APL6 S1 5ʹ-GAA​AGG​TAA​ACC​AAA​GAC​AGC​AAA​CAA​TCG​AAA​AGT​GGA​CAA​CCA​GCA​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
APL6 S2 5ʹ-CCT​ATA​TCT​ATA​TCT​ATA​AAT​ATA​CAT​ATT​TAT​ATA​GTA​TTA​TCT​CCG​AGT​TCT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3ʹ 
SLM4 S1 5ʹ-AAA​AGG​CAG​TTA​TCA​GCA​GCA​AAC​GGT​ATC​CAA​AAT​ATT​GAA​GCA​ATA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
SLM4 S2 5ʹ-CGG​AGA​CGC​ATG​AAA​AGG​TGT​GGC​CTC​GAT​ACA​TAT​TGT​TAA​ACC​CAT​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
NCR1 S2 5ʹ-TTT​TAC​CTA​TTT​TTT​CAC​TAC​GTA​AAA​TAT​AGT​ATA​ATC​TGC​TAT​GGC​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
NCR1 S3 5ʹ-CAT​TGT​TTG​GTG​GTG​AAA​GCT​ATA​GGG​ACG​ATT​CCA​TCG​AAG​CAG​AAG​ATC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APE1 S1 5ʹ-TTA​GTG​CAA​TTG​TAG​AAA​CCT​GCA​CAA​CCA​ACA​AAA​TTA​AGA​AAA​AAA​GAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APE1 S2 5ʹ-AAA​AGG​ATA​AAG​AAA​CAG​AAA​TCA​AAA​GAA​ATA​AAA​AGA​GTG​TGG​CAA​AAT​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
YPK9 S1 5ʹ-TAA​AAA​GGA​GCC​CAG​ACT​TAC​TGA​TAG​ATC​TTG​CAT​ATA​CTC​CGG​TAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
YPK9 S4 5ʹ-TTC​CTT​TCA​CTC​CTT​TGG​CCA​TGT​TGA​ATT​TGG​TTT​GAA​GAG​GGA​ATA​TCC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
pRS405 for 5ʹ-TAT​TTA​TAG​TAC​TTC​ATT​CAG​CCC​GGG​GGA​TCC​AC-3ʹ 
pRS405 rev 5ʹ-CGG​ATA​CTT​TTT​AGT​TTC​AGG​AAT​TCG​ATA​TCA​AGC​TTA​TCG-3ʹ 
VPS10 for 5ʹ-TTG​ATA​TCG​AAT​TCC​TGA​AAC​TAA​AAA​GTA​TCC​GCC​TG-3ʹ 
VPS10 rev 5ʹ-GTG​GAT​CCC​CCG​GGC​TGA​ATG​AAG​TAC​TAT​AAA​TAT​TAA​AGT​ACG-3ʹ 
VPS10 D1 del for 5ʹ-AAG​GGA​ACG​AAT​GAT​AAA​GAA​ATA-3ʹ 
VPS10 D1 del rev 5ʹ-TGC​TCC​AGC​ACC​AGC​ACC-3ʹ 
VPS10 D2 del for 5ʹ-CGC​CAT​CTA​ACG​AAA​ACC​AG-3ʹ 
VPS10 D2 del rev 5ʹ-CTT​GAA​ATC​AAA​TTT​GTT​TTC​TGT​AAC​C-3ʹ 
NPC2 S1 5ʹ-CAT​AAC​CAT​ATT​AAA​TCT​TCT​CCT​TCA​AAG​CTA​GCA​CGC​CTT​CCA​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
VAC7 S1 5ʹ-CCT​TAT​CGT​TTC​ATC​TCA​GGC​AAG​TTA​AAG​CAT​TTG​GGA​AAC​GTG​CTA​GAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
VAC7 S4 5ʹ-CGG​GTG​CCT​CAA​CTG​TTT​CTG​TCT​CTA​CAG​TGA​GCT​TTC​TAT​CTT​CTT​CTG​TCA​TCG​ATG​AAT​TCT​CTG​TCG-3ʹ 
SLM4 S3 5ʹ-AAA​GAG​CTA​TGA​GAG​AGT​TGA​CTG​ATT​TGT​TTG​GCT​ACA​AGC​TAG​GTC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
MUP1 S2 5ʹ-GTT​CAT​ACG​TGA​TTA​TAA​GAA​TCG​AGA​TGA​GAT​GGT​AAG​TAC​CTT​TTT​GGT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
MUP1 S3 5ʹ-CGT​TAT​TGA​AAC​GAA​TAT​AAT​CGA​ACA​TTA​CAA​AAG​TGA​ACA​AGA​AAA​ATC​GCT​GCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
YCK3 S1 5ʹ-GTG​GTA​TCT​CAT​TCT​GAA​GAA​AAA​GTG​TAA​AAG​GAC​GAT​AAG​GAA​AGA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
YCK3 S4 5ʹ-GGT​CCT​ACA​GCA​TAA​TGA​ATA​CCT​ACA​ATG​TGT​TGT​GAA​GAT​CGT​TGG​GAC​ATC​GAT​GAA​TTC​TCT​GTC-3ʹ 
Ncr1 S2 Cterm cytosol del 5ʹ-ATC​TTC​TGC​TTC​GAT​GGA​ATC​GTC​CCT​ATA​GCT​TTC​ACC​ACC​AAA​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
Ncr1 S3 Cterm cytosl del 5ʹ-CAT​TGT​AGC​AGC​ATT​GCA​TGC​TCT​CCT​ATT​TTT​ACC​TGC​TTT​ACT​TTC​ATC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG15 S1 5ʹ-AAC​TGA​TCT​AGG​CAT​TAC​AAT​TAA​AGG​AAA​CAA​GGG​AAA​TAT​TCT​ATT​GAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG15 S4 5ʹ-CCT​AGA​TGC​AAA​GGA​GAA​GCA​AAT​CTC​TTT​CTT​GAA​GGG​CTT​TTA​TGC​AAC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
Atg15 S1 Nterm cytosl del 5ʹ-TTG​CAT​AAA​AGC​CCT​TCA​AGA​AAG​AGA​TTT​GCT​TCT​CCT​TTG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
Atg15 S4 Nterm cytosl del 5ʹ-GCA​ATA​AGG​CAG​AGC​ACT​GTA​AGC​GTT​AGA​ATG​CAT​CCT​AGA​TGC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
VPS27 S1 5ʹ-TTG​CTA​AGG​TGA​ATG​AGT​AGT​GAG​TAA​AGA​ACT​AAG​AAC​AGT​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
VPS27 S2 5ʹ-GCG​CTA​GGT​TTC​TTT​TTA​CAA​ATA​CAT​AGA​AAA​GGC​TAC​AAT​ATT​AAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
ATG15 S2 5ʹ-CGC​ATA​GGC​CCT​AAA​ACA​ACA​CTA​GGG​TCA​TAA​TAG​ATG​TAT​GGG​TCT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
ATG27 S1 5ʹ-GAG​ATC​TTC​AAT​CGA​TGC​GAT​AGA​TAA​AGG​TAA​GGA​AAG​CTT​TCA​CGA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG27 S2 5ʹ-TAG​CAC​TGC​TGT​TGC​AAA​AAT​ATC​GAA​TTG​TAA​GCC​AGT​AAA​CTT​ATT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
ATG4 S1 5ʹ-GTA​GAT​GAA​GAA​TGG​ACG​ACT​TCT​TAT​CAC​GTA​TAG​GAG​TGA​TAT​ACA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG4 S2 5ʹ-GGG​AAT​ATA​TTA​AAA​CAA​GTA​TAT​ATG​CTT​ATG​AAC​TAG​TGA​ATT​CCT​TAC​ACT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3ʹ 
Primer Sequence 
NPC2 S3 5ʹ-AGG​ATG​ATC​TGA​TCA​CTT​GCT​TAA​CCG​GAG​AAG​TCA​TCT​TCC​CAC​CAA​GGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
NPC2 S2 5ʹ-GAA​CGA​GAA​GGG​AAA​TAA​ACA​CGG​ATC​AAT​GAG​TTG​TAT​GAA​TCA​GAT​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
VPS10 S1 5ʹ-CTG​TAT​ATC​TGG​AAA​AGC​CCT​GAA​GTG​TCC​AGT​AGT​CAT​CAC​ACG​TTA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
VPS10 S2 5ʹ-GTT​TAT​GAA​AAG​TAT​ATG​GAA​TTA​TCT​ACT​CTA​TGT​AAA​GTA​ATC​TCT​CTA​ATC​GAT​GAA​TTC​GAG​CTC​G-3ʹ 
CPY S2 5ʹ-TAA​AGC​GTG​TAT​GTG​TAG​GCA​TAC​CGT​TTT​TAT​TAT​CAG​CTA​CGA​TCG​AAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
CPY S3 5ʹ-AAA​ACG​CCT​TAA​GTA​TGG​TTA​ACG​AAT​GGA​TCC​ACG​GTG​GTT​TCT​CCT​TAC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APM3 S1 5ʹ-GAC​TTG​GGC​AAC​AAC​AGA​GGC​TGT​AAA​CCT​TAC​CAA​CCC​AAC​CAA​AAT​AGA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APM3 S2 5ʹ-CTC​ATT​ATA​TTC​TAT​TTA​GTT​TCG​CAT​GGA​ATT​TCA​AGT​ACA​CAT​ATA​ACT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3ʹ 
APM3 for 5ʹ-GAT​ATC​GAA​TTC​CTG​GAG​GGG​TAG​AAG​TCG​CTG-3ʹ 
APM3 rev 5ʹ-GGA​TCC​CCC​GGG​CTG​TGC​GGA​AGT​CTT​CCC​TAA​G-3ʹ 
pRS APM3 for 5ʹ-AGG​GAA​GAC​TTC​CGC​ACA​GCC​CGG​GGG​ATC​CAC-3ʹ 
pRS APM3 rev 5ʹ-GCG​ACT​TCT​ACC​CCT​CCA​GGA​ATT​CGA​TAT​CAA​GCT​TAT​CG-3ʹ 
APM3 D217A for 5ʹ-GCT​TTA​TGT​GGC​TTT​GTT​AGA​AAC-3ʹ 
APM3 D217A rev 5ʹ-TCA​TTA​TTC​TCA​TGT​TTG​CTA​G-3ʹ 
APM3 G469K for 5ʹ-GCT​GTA​CAA​GAA​GGC​AAA​GTA​TAA​GAC-3ʹ 
APM3 G469K rev 5ʹ-TTT​GTA​TTT​TTG​CCA​ATT​GTT​AAT​C-3ʹ 
APS3 S1 5ʹ-CTA​TAC​CAG​ATC​TGG​GTT​ACA​GAG​GCC​AGA​ACA​CAT​ATA​ACG​TTA​CAC​AAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
APS3 S2 5ʹ-CAG​ATC​TTG​ACG​AAC​GAA​AAT​AAC​CAT​CAT​CCG​ATT​AAT​TTG​TTT​GTT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
APS3 for 5ʹ-TGA​TAT​CGA​ATT​CCT​GCT​TTT​GAG​TAT​GAG​GAG​TTC​CAC-3ʹ 
APS3 rev 5ʹ-TGG​ATC​CCC​CGG​GCT​GGT​GGA​TTT​TGC​CAT​TTC​TAA​AGA​G-3ʹ 
pRS APS3 for 5ʹ-AAA​TGG​CAA​AAT​CCA​CCA​GCC​CGG​GGG​ATC​CAC-3ʹ 
pRS APS3 rev 5ʹ-TCC​TCA​TAC​TCA​AAA​GCA​GGA​ATT​CGA​TAT​CAA​GCT​TAT​CG-3ʹ 
APS3 V106D for 5ʹ-CCA​AAC​TTT​TGA​CGA​ATC​ATT​GGA​C-3ʹ 
APS3 V106D rev 5ʹ-ATC​AGA​TCT​AAT​ATG​GCC-3ʹ 
APS3 L121S for 5ʹ-TGA​ACT​TGA​TTC​GAT​TTT​TAA​CTG​G-3ʹ 
APS3 L121S rev 5ʹ-TTG​ACT​TCA​GTA​AAA​CAA​CG-3ʹ 
GGA1 S1 5ʹ-GGG​ACA​AGT​CAC​TAC​TTC​AAG​TAT​AAC​CCA​GAC​AAG​AGT​CTT​TTA​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
GGA2 S2 5ʹ-ATA​TAA​TAT​GGC​ATC​TAC​TTT​TTT​TTC​AAC​TTC​TCT​ACC​GAA​TTT​GAT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
GGA2 S1 5ʹ-AAG​AGC​AAC​ATA​ACT​CAT​AGC​TGA​ATT​GCT​AAT​CGT​GAT​ACT​GCA​TCA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
GGA2 S2 5ʹ-AAA​ATA​TAC​ATA​GAG​AAG​AGA​AAG​GAT​TGA​TAA​GAA​ACG​CCA​GAG​GAT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
VPS45 S1 5ʹ-GAC​TTG​GTT​TTG​AGT​TAA​GGC​CAT​CTT​TTA​CTG​TAT​AGA​ACA​AAG​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
VPS45 S2 5ʹ-GAT​TTA​TGC​CTC​ATA​TAT​AAA​ATA​GAA​TTT​TAG​AAT​AAG​ATA​ATC​CTT​ATT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
APL5 S1 5ʹ-GAA​GTG​GGA​AAG​GCA​GTT​GCC​ACA​ACC​AGA​AGA​GCA​TAA​CAT​ATT​GTC​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
APL5 S2 5ʹ-CGT​AAT​CGC​TGT​CAT​TGA​GAA​TTC​ACG​ACT​TCG​ATT​TTT​TTA​TCG​ATC​GAT​GAA​TTC​GAG​CTC​G-3ʹ 
ATG19 S1 5ʹ-TTG​CGG​CGG​CAC​TTG​CTT​CAG​TAA​CGC​CCA​AAG​GAG​AGT​TCT​GGT​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG19 S2 5ʹ-TGT​GTA​TGT​GAA​AAG​GTA​CTC​ATT​GCT​GTA​TAA​AAA​TAG​AGT​TTG​ACC​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
LAP3 S1 5ʹ-CGT​TTA​GAA​GCC​ACC​TTT​TAC​GAG​CAC​CAC​AAA​TTG​TTT​TAA​AAA​GAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
LAP3 S4 5ʹ-TGA​AAC​TCT​TTG​TTC​CAA​GAG​TTG​ATC​TTA​CTG​ATA​TCG​ATG​GAA​GAG​GAC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
VPH1 S2 5ʹ-TAT​TTA​ATG​AAG​TAC​TTA​AAT​GTT​TCG​CTT​TTT​TTA​AAA​GTC​CTC​AAA​ATA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
VPH1 S3 5ʹ-GTA​TAA​AGA​CAT​GGA​AGT​CGC​TGT​TGC​TAG​TGC​AAG​CTC​TTC​CGC​TTC​AAG​CCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
APL6 S1 5ʹ-GAA​AGG​TAA​ACC​AAA​GAC​AGC​AAA​CAA​TCG​AAA​AGT​GGA​CAA​CCA​GCA​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
APL6 S2 5ʹ-CCT​ATA​TCT​ATA​TCT​ATA​AAT​ATA​CAT​ATT​TAT​ATA​GTA​TTA​TCT​CCG​AGT​TCT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3ʹ 
SLM4 S1 5ʹ-AAA​AGG​CAG​TTA​TCA​GCA​GCA​AAC​GGT​ATC​CAA​AAT​ATT​GAA​GCA​ATA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
SLM4 S2 5ʹ-CGG​AGA​CGC​ATG​AAA​AGG​TGT​GGC​CTC​GAT​ACA​TAT​TGT​TAA​ACC​CAT​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
NCR1 S2 5ʹ-TTT​TAC​CTA​TTT​TTT​CAC​TAC​GTA​AAA​TAT​AGT​ATA​ATC​TGC​TAT​GGC​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
NCR1 S3 5ʹ-CAT​TGT​TTG​GTG​GTG​AAA​GCT​ATA​GGG​ACG​ATT​CCA​TCG​AAG​CAG​AAG​ATC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APE1 S1 5ʹ-TTA​GTG​CAA​TTG​TAG​AAA​CCT​GCA​CAA​CCA​ACA​AAA​TTA​AGA​AAA​AAA​GAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
APE1 S2 5ʹ-AAA​AGG​ATA​AAG​AAA​CAG​AAA​TCA​AAA​GAA​ATA​AAA​AGA​GTG​TGG​CAA​AAT​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
YPK9 S1 5ʹ-TAA​AAA​GGA​GCC​CAG​ACT​TAC​TGA​TAG​ATC​TTG​CAT​ATA​CTC​CGG​TAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
YPK9 S4 5ʹ-TTC​CTT​TCA​CTC​CTT​TGG​CCA​TGT​TGA​ATT​TGG​TTT​GAA​GAG​GGA​ATA​TCC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
pRS405 for 5ʹ-TAT​TTA​TAG​TAC​TTC​ATT​CAG​CCC​GGG​GGA​TCC​AC-3ʹ 
pRS405 rev 5ʹ-CGG​ATA​CTT​TTT​AGT​TTC​AGG​AAT​TCG​ATA​TCA​AGC​TTA​TCG-3ʹ 
VPS10 for 5ʹ-TTG​ATA​TCG​AAT​TCC​TGA​AAC​TAA​AAA​GTA​TCC​GCC​TG-3ʹ 
VPS10 rev 5ʹ-GTG​GAT​CCC​CCG​GGC​TGA​ATG​AAG​TAC​TAT​AAA​TAT​TAA​AGT​ACG-3ʹ 
VPS10 D1 del for 5ʹ-AAG​GGA​ACG​AAT​GAT​AAA​GAA​ATA-3ʹ 
VPS10 D1 del rev 5ʹ-TGC​TCC​AGC​ACC​AGC​ACC-3ʹ 
VPS10 D2 del for 5ʹ-CGC​CAT​CTA​ACG​AAA​ACC​AG-3ʹ 
VPS10 D2 del rev 5ʹ-CTT​GAA​ATC​AAA​TTT​GTT​TTC​TGT​AAC​C-3ʹ 
NPC2 S1 5ʹ-CAT​AAC​CAT​ATT​AAA​TCT​TCT​CCT​TCA​AAG​CTA​GCA​CGC​CTT​CCA​AAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
VAC7 S1 5ʹ-CCT​TAT​CGT​TTC​ATC​TCA​GGC​AAG​TTA​AAG​CAT​TTG​GGA​AAC​GTG​CTA​GAT​GCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
VAC7 S4 5ʹ-CGG​GTG​CCT​CAA​CTG​TTT​CTG​TCT​CTA​CAG​TGA​GCT​TTC​TAT​CTT​CTT​CTG​TCA​TCG​ATG​AAT​TCT​CTG​TCG-3ʹ 
SLM4 S3 5ʹ-AAA​GAG​CTA​TGA​GAG​AGT​TGA​CTG​ATT​TGT​TTG​GCT​ACA​AGC​TAG​GTC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
MUP1 S2 5ʹ-GTT​CAT​ACG​TGA​TTA​TAA​GAA​TCG​AGA​TGA​GAT​GGT​AAG​TAC​CTT​TTT​GGT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
MUP1 S3 5ʹ-CGT​TAT​TGA​AAC​GAA​TAT​AAT​CGA​ACA​TTA​CAA​AAG​TGA​ACA​AGA​AAA​ATC​GCT​GCG​TAC​GCT​GCA​GGT​CGA​C-3ʹ 
YCK3 S1 5ʹ-GTG​GTA​TCT​CAT​TCT​GAA​GAA​AAA​GTG​TAA​AAG​GAC​GAT​AAG​GAA​AGA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
YCK3 S4 5ʹ-GGT​CCT​ACA​GCA​TAA​TGA​ATA​CCT​ACA​ATG​TGT​TGT​GAA​GAT​CGT​TGG​GAC​ATC​GAT​GAA​TTC​TCT​GTC-3ʹ 
Ncr1 S2 Cterm cytosol del 5ʹ-ATC​TTC​TGC​TTC​GAT​GGA​ATC​GTC​CCT​ATA​GCT​TTC​ACC​ACC​AAA​CAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
Ncr1 S3 Cterm cytosl del 5ʹ-CAT​TGT​AGC​AGC​ATT​GCA​TGC​TCT​CCT​ATT​TTT​ACC​TGC​TTT​ACT​TTC​ATC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG15 S1 5ʹ-AAC​TGA​TCT​AGG​CAT​TAC​AAT​TAA​AGG​AAA​CAA​GGG​AAA​TAT​TCT​ATT​GAA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG15 S4 5ʹ-CCT​AGA​TGC​AAA​GGA​GAA​GCA​AAT​CTC​TTT​CTT​GAA​GGG​CTT​TTA​TGC​AAC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
Atg15 S1 Nterm cytosl del 5ʹ-TTG​CAT​AAA​AGC​CCT​TCA​AGA​AAG​AGA​TTT​GCT​TCT​CCT​TTG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
Atg15 S4 Nterm cytosl del 5ʹ-GCA​ATA​AGG​CAG​AGC​ACT​GTA​AGC​GTT​AGA​ATG​CAT​CCT​AGA​TGC​ATC​GAT​GAA​TTC​TCT​GTC​G-3ʹ 
VPS27 S1 5ʹ-TTG​CTA​AGG​TGA​ATG​AGT​AGT​GAG​TAA​AGA​ACT​AAG​AAC​AGT​ATG​CGT​ACG​CTG​CAG​GTC​GAC-3ʹ 
VPS27 S2 5ʹ-GCG​CTA​GGT​TTC​TTT​TTA​CAA​ATA​CAT​AGA​AAA​GGC​TAC​AAT​ATT​AAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
ATG15 S2 5ʹ-CGC​ATA​GGC​CCT​AAA​ACA​ACA​CTA​GGG​TCA​TAA​TAG​ATG​TAT​GGG​TCT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
ATG27 S1 5ʹ-GAG​ATC​TTC​AAT​CGA​TGC​GAT​AGA​TAA​AGG​TAA​GGA​AAG​CTT​TCA​CGA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG27 S2 5ʹ-TAG​CAC​TGC​TGT​TGC​AAA​AAT​ATC​GAA​TTG​TAA​GCC​AGT​AAA​CTT​ATT​TAA​TCG​ATG​AAT​TCG​AGC​TCG-3ʹ 
ATG4 S1 5ʹ-GTA​GAT​GAA​GAA​TGG​ACG​ACT​TCT​TAT​CAC​GTA​TAG​GAG​TGA​TAT​ACA​TGC​GTA​CGC​TGC​AGG​TCG​AC-3ʹ 
ATG4 S2 5ʹ-GGG​AAT​ATA​TTA​AAA​CAA​GTA​TAT​ATG​CTT​ATG​AAC​TAG​TGA​ATT​CCT​TAC​ACT​AAT​CGA​TGA​ATT​CGA​GCT​CG-3ʹ 
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