| Description . | Protein coverage . | Sequence . | Glucose . | No glucose . |
|---|---|---|---|---|
| Ins1C | Ins1C:37–56 | PQVEQLELGGSPGDLQTLAL | 22, 18 | 8 |
| Ins2B | Ins2B:9–23 | SHLVEALYLVCGERG | 10, 11 | 6 |
| Ins1C | Ins1C:51–61 | LQTLALEVARQ | 7, 6 | 0 |
| Insulin-2 C-peptide | Ins2C:37–47 | PQVAQLELGGG | 2, 2 | 1 |
| Vitamin D–binding protein | Gc:389–405 | SPLLKRQLTSFIEKGQE | 1, 1 | 0 |
| 60-kD heat shock protein | Hspd1:164–183 | TPEEIAQVATISANGDKDIG | 0, 2 | 0 |
| Zinc transporter 8 | ZnT8:313–322 | ILSVHVATAA | 0, 1 | 0 |
| Insulin-2 C-peptide | Ins2C:48–58 | PGAGDLQTLAL | 0, 1 | 0 |
| Description . | Protein coverage . | Sequence . | Glucose . | No glucose . |
|---|---|---|---|---|
| Ins1C | Ins1C:37–56 | PQVEQLELGGSPGDLQTLAL | 22, 18 | 8 |
| Ins2B | Ins2B:9–23 | SHLVEALYLVCGERG | 10, 11 | 6 |
| Ins1C | Ins1C:51–61 | LQTLALEVARQ | 7, 6 | 0 |
| Insulin-2 C-peptide | Ins2C:37–47 | PQVAQLELGGG | 2, 2 | 1 |
| Vitamin D–binding protein | Gc:389–405 | SPLLKRQLTSFIEKGQE | 1, 1 | 0 |
| 60-kD heat shock protein | Hspd1:164–183 | TPEEIAQVATISANGDKDIG | 0, 2 | 0 |
| Zinc transporter 8 | ZnT8:313–322 | ILSVHVATAA | 0, 1 | 0 |
| Insulin-2 C-peptide | Ins2C:48–58 | PGAGDLQTLAL | 0, 1 | 0 |
MHC-II–bound peptides were isolated from blood leukocytes from NOD mice after glucose challenge and analyzed by LC-MS/MS. The table summarizes all the peptide families derived from pancreatic β cell proteins. The numbers of the individual peptides belonging to each family identified in mice subjected to a glucose challenge (two independent experiments) relative to those without glucose challenge (a single experiment) are shown. The two experiments of the glucose challenge condition used 3 × 107 total blood leukocytes from 20 mice and 7.5 × 107 cells from 40 mice, respectively. The experiment of the no glucose condition used 3 × 107 cells isolated from 19 mice. Columns are as follows: Description, protein name; Protein coverage, gene name and amino acid residues covered; Sequence, amino acid sequence encompassing all peptides identified for a particular family; Glucose, the number of peptides identified in the specified family for the two biological replicates of the glucose challenge experiments; and No glucose, peptides identified in the mice that did not receive glucose.