Table 2.
Sequence analysis of the V2 and V31 rearrangements on opposite alleles in V2R/+/V31+/R T cell hybridomas
N/PPotential DβN/PStatus
Clone 1 V2 AGCAGCCAAGA TA A T CCGGGCAG 2.2 Out-of-frame 
 V31 GCCTGGAGTCT  AC  CCAACGAAAGA 1.4 In-frame 
Clone 23 V2 AGCAGCCAAGA TGG  GAACAG 2.7 In-frame 
 V31 The V31 rearrangement was not identified 
Clone 54 V2 AGCAGC CAA GAC  AACACCGGGCAG 2.2 In-frame 
 V31 GCCTGGAGTCT GC G TGAACA2.7 Out-of-frame 
Clone 55 V2 AGCAGC T T  CTCCTATGAACAG 2.7 In-frame 
 V31 GCCTGGAGTCTC  GGGACAGGGGG GCGG GAACAG 2.7 In-frame 
Clone 59 V2 AGCAGCCAAGA TC GG  AGTCAAAACACCTTG 2.4 In-frame 
 V31 GCC GGC  CACCTTG 2.4 Out-of-frame 
Clone 62 V2 The V2(Dβ)Jβ2 rearrangement was not cloned and sequenced 
 V31 V31-to-5′Dβ1 RSS hybrid join, deleting all subsequent Jβ1-Cβ1 and Dβ2/Jβ2-Cβ2 sequences 
Clone 90 V2 AGCAGC TT CT CTATGAACAG 2.7 In-frame 
 V31 GCCTGGAGTCCCCT CTGGGGGG TGCAGAAA CGCTGTATTTTGGC 2.3 In-frame 
Clone 105 V2 AGCAGCCA CCTCC GGGACTGGGGC TATGA ACAG 2.7 In-frame 
 V31 V31 rearranged to the Vβ coding sequence of an out of frame V29(Dβ)Jβ2.7 rearrangement Out-of-frame 
N/PPotential DβN/PStatus
Clone 1 V2 AGCAGCCAAGA TA A T CCGGGCAG 2.2 Out-of-frame 
 V31 GCCTGGAGTCT  AC  CCAACGAAAGA 1.4 In-frame 
Clone 23 V2 AGCAGCCAAGA TGG  GAACAG 2.7 In-frame 
 V31 The V31 rearrangement was not identified 
Clone 54 V2 AGCAGC CAA GAC  AACACCGGGCAG 2.2 In-frame 
 V31 GCCTGGAGTCT GC G TGAACA2.7 Out-of-frame 
Clone 55 V2 AGCAGC T T  CTCCTATGAACAG 2.7 In-frame 
 V31 GCCTGGAGTCTC  GGGACAGGGGG GCGG GAACAG 2.7 In-frame 
Clone 59 V2 AGCAGCCAAGA TC GG  AGTCAAAACACCTTG 2.4 In-frame 
 V31 GCC GGC  CACCTTG 2.4 Out-of-frame 
Clone 62 V2 The V2(Dβ)Jβ2 rearrangement was not cloned and sequenced 
 V31 V31-to-5′Dβ1 RSS hybrid join, deleting all subsequent Jβ1-Cβ1 and Dβ2/Jβ2-Cβ2 sequences 
Clone 90 V2 AGCAGC TT CT CTATGAACAG 2.7 In-frame 
 V31 GCCTGGAGTCCCCT CTGGGGGG TGCAGAAA CGCTGTATTTTGGC 2.3 In-frame 
Clone 105 V2 AGCAGCCA CCTCC GGGACTGGGGC TATGA ACAG 2.7 In-frame 
 V31 V31 rearranged to the Vβ coding sequence of an out of frame V29(Dβ)Jβ2.7 rearrangement Out-of-frame 

Sequences are underlined every three nucleotides to orient the reading frame. Dβ nucleotides in bold are from Dβ1, italicized nucleotides are from Dβ2, and for V2 rearrangements, unformatted nucleotides could be from either.

or Create an Account

Close Modal
Close Modal