Plasmids
| Plasmid . | Description . | Source or reference . |
|---|---|---|
| p115 | pRS316 | Sikorski and Hieter (1989) |
| p117 | pRS313 | Sikorski and Hieter (1989) |
| p2492 | pRS426-MET25P-FPS1-Flag | Beese et al. (2009) |
| p2823 | pAG32 | Goldstein and McCusker (1999) |
| p3090 | YCplac111-HOG1-3HA | F. Posas |
| p3091 | YCplac111 | F. Posas |
| p3121 | pYEp181-MET25P-FPS1-Myc | Lee et al. (2013) |
| p3148 | pRS316-3HA-ADH1T | Lee et al. (2013) |
| p3151 | pRS316-MET25P-RGC2-3HA | Lee et al. (2013) |
| p3155 | pRS316-MET25P-RGC2-S75A, S344A, T808A, S827A, S948A, S1021A, S1035A-3HA (7A) | Lee et al. (2013) |
| p3182 | pRS316-RGC2-3HA | Lee et al. (2013) |
| p3199 | pRS413-CFPC | Lipatova et al. (2012) |
| p3200 | pRS415-CFPN | Lipatova et al. (2012) |
| p3216 | pRS413-CFPC-FPS1 (BiFC) | Lee et al. (2013) |
| p3217 | pRS413-CFPC-HOG1 (BiFC) | Lee et al. (2013) |
| p3225 | pRS426-HOG1-3HA | Lee and Levin (2018) |
| p3460 | pYEP181-MET25P-TRM112 | Lee and Levin (2018) |
| p3467 | GAL1P-GPD1-TAP (6HIS-HA-ZZ) | Open biosystems |
| p3470 | pRS316-MET25P-ACR3-3HA | This study |
| p3471 | pRS313-MET25P-RGC2-3HA | This study |
| p3577 | YCplac111-HOG1-C38S-3HA | This study |
| p3578 | YCplac111-HOG1-C156S-3HA | This study |
| p3579 | YCplac111-HOG1-C205S-3HA | This study |
| p3580 | YCplac111-HOG1-C38S, C156S-3HA | This study |
| p3581 | YCplac111-HOG1-C156S, C205S-3HA | This study |
| p3582 | YCplac111-HOG1-C38S, C156S, C205S-3HA | This study |
| p3583 | YCplac111-HOG1-C38S, C156S, C161S, C205S-3HA | This study |
| p3584 | pRS413-CFPC-RGC2 (BiFC) | This study |
| p3585 | pRS415-ACR3-CFPN (BiFC) | This study |
| p3586 | pRS415-ACR3-Δ307-334-CFPN (BiFC) | This study |
| p3587 | pRS316-ACR3-3HA | This study |
| p3588 | pRS316-ACR3-Δ307-334-3HA | This study |
| p3589 | pRS316-ACR3-C316SC, 318S-3HA | This study |
| p3590 | pRS316-ACR3-C316S, C318S, C333S-3HA | This study |
| p3591 | pRS316-MET25P-ACR3-C169S-3HA | This study |
| p3592 | pRS316-MET25P-ACR3-C333S-3HA | This study |
| p3593 | pRS316-MET25P-ACR3-C316S, C318S-3HA | This study |
| p3594 | pRS316-MET25P-ACR3-C316S, C318S, C333S-3HA | This study |
| p3628 | pRS415-ACR3-C316S, C318S, C333S-CFPN (BiFC) | This study |
| p3629 | pRS313-MET25P-MTQ2-6xHIS | This study |
| Plasmid . | Description . | Source or reference . |
|---|---|---|
| p115 | pRS316 | Sikorski and Hieter (1989) |
| p117 | pRS313 | Sikorski and Hieter (1989) |
| p2492 | pRS426-MET25P-FPS1-Flag | Beese et al. (2009) |
| p2823 | pAG32 | Goldstein and McCusker (1999) |
| p3090 | YCplac111-HOG1-3HA | F. Posas |
| p3091 | YCplac111 | F. Posas |
| p3121 | pYEp181-MET25P-FPS1-Myc | Lee et al. (2013) |
| p3148 | pRS316-3HA-ADH1T | Lee et al. (2013) |
| p3151 | pRS316-MET25P-RGC2-3HA | Lee et al. (2013) |
| p3155 | pRS316-MET25P-RGC2-S75A, S344A, T808A, S827A, S948A, S1021A, S1035A-3HA (7A) | Lee et al. (2013) |
| p3182 | pRS316-RGC2-3HA | Lee et al. (2013) |
| p3199 | pRS413-CFPC | Lipatova et al. (2012) |
| p3200 | pRS415-CFPN | Lipatova et al. (2012) |
| p3216 | pRS413-CFPC-FPS1 (BiFC) | Lee et al. (2013) |
| p3217 | pRS413-CFPC-HOG1 (BiFC) | Lee et al. (2013) |
| p3225 | pRS426-HOG1-3HA | Lee and Levin (2018) |
| p3460 | pYEP181-MET25P-TRM112 | Lee and Levin (2018) |
| p3467 | GAL1P-GPD1-TAP (6HIS-HA-ZZ) | Open biosystems |
| p3470 | pRS316-MET25P-ACR3-3HA | This study |
| p3471 | pRS313-MET25P-RGC2-3HA | This study |
| p3577 | YCplac111-HOG1-C38S-3HA | This study |
| p3578 | YCplac111-HOG1-C156S-3HA | This study |
| p3579 | YCplac111-HOG1-C205S-3HA | This study |
| p3580 | YCplac111-HOG1-C38S, C156S-3HA | This study |
| p3581 | YCplac111-HOG1-C156S, C205S-3HA | This study |
| p3582 | YCplac111-HOG1-C38S, C156S, C205S-3HA | This study |
| p3583 | YCplac111-HOG1-C38S, C156S, C161S, C205S-3HA | This study |
| p3584 | pRS413-CFPC-RGC2 (BiFC) | This study |
| p3585 | pRS415-ACR3-CFPN (BiFC) | This study |
| p3586 | pRS415-ACR3-Δ307-334-CFPN (BiFC) | This study |
| p3587 | pRS316-ACR3-3HA | This study |
| p3588 | pRS316-ACR3-Δ307-334-3HA | This study |
| p3589 | pRS316-ACR3-C316SC, 318S-3HA | This study |
| p3590 | pRS316-ACR3-C316S, C318S, C333S-3HA | This study |
| p3591 | pRS316-MET25P-ACR3-C169S-3HA | This study |
| p3592 | pRS316-MET25P-ACR3-C333S-3HA | This study |
| p3593 | pRS316-MET25P-ACR3-C316S, C318S-3HA | This study |
| p3594 | pRS316-MET25P-ACR3-C316S, C318S, C333S-3HA | This study |
| p3628 | pRS415-ACR3-C316S, C318S, C333S-CFPN (BiFC) | This study |
| p3629 | pRS313-MET25P-MTQ2-6xHIS | This study |