Table 1.
Primers and oligomers used for cloning of constructs
ConstructForward primer (5′ to 3′)Reverse primer (5′ to 3′)
pLPCX-Cab45-5pXA T131: GGA​TCA​TGG​AGA​AGG​CGG​CCG​AGC​ACT​TC S99: AGC​TTC​CTC​CGG​GCC​CGC​CGC​GGC​TC 
T142: GAG​GCC​ATG​GAG​GAG​GCC​AAG​ACA​CAC​TTC​CG 
S193: TCA​AAG​TGG​ATG​AGG​AAG​CAC​AGG​AAG​TCC​TGG​A 
S349: CGA​GTT​CTT​CAC​GGG​CGC​CAA​GCT​GGT​GGA​CTA​C 
pLPCX-Cab45-5pXE T131: GGA​TCA​TGG​AGA​AGG​AAG​CCG​AGC​ACT​TC S99: AGC​TTC​CTC​CGT​TCC​CGC​CGC​GGC​TC 
T142: GAG​GCC​ATG​GAG​GAG​GAA​AAG​ACA​CAC​TTC​CG 
S193: TCA​AAG​TGG​ATG​AGG​AAG​AAC​AGG​AAG​TCC​TGG​A 
S349: CGA​GTT​CTT​CAC​GGG​CGA​AAA​GCT​GGT​GGA​CTA​C 
pB-T-PAF-Cab45-WT/5pXA/5pXE Fragment1: GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​ATG​GCT​ACA​GGC​TCC​CGG Fragment1: GGT​TGG​CAG​GCC​GGC​ATT​CAC​CTC​C 
Fragment2: GGA​GGT​GAA​TGC​CGG​CCT​GCC​AAC​C Fragment2: CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​TTA​AAA​CTC​CTC​GTG​CAC​GCT​GCG 
pBT-PAF-GFP-Cab45-WT/5pXA/5pXE Fragment1: GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TGG​CTA​CAG​GCT​CCC​G Fragment1: CCG​CTT​CCA​CCT​CCA​CCA​GAT​CTG​TGA​TGA​TGA​TGG​TGA​TGA​AGC​TTT​GTT​C 
Fragment2: CTG​GTG​GAG​GTG​GAA​GCG​GTA​GCA​AAG​GAG​AAG​AAC​TTT​TCA​CTG​G Fragment2: CTT​TGG​AAG​TAC​AGG​TTC​TCG​GAT​CCG​GCC​GGC​CGA​CCT​CCA​CCT​TTG​TAG​AGC​TC 
Fragment3: GAG​GTC​GGC​CGG​CCG​GAT​CCG​AGA​ACC​TGT​ACT​TCC​AAA​GTG​GCG​CGC​CAC​GGC​CTG​CCA​ACC​AC Fragment3: GGA​TCA​GTT​ATC​TAT​GCG​GCC​GCT​CTA​GAT​TAA​AAC​TCC​TCG​TGC​ACG​C 
pLPCX-SS-EGFP-Cab45-5pXA/5pXE Fragment1: CGT​GAC​CGC​CGC​CCC​GGA​ATT​CCG​GCC​TGC​CAA​CCA​CTC​G Fragment1: TTT​ATC​GAT​GTT​TGG​CCG​AGG​CGA​CCG​GTT​TAA​AAC​TCC​TCG​TGC​ACG​CTG​CG 
pLPCX-Fam20C-HA Fragment1: CGT​AGA​TCT​ATG​AAG​ATG​ATG​CTG​GTG​CGC​CG Fragment1: GCA​CAA​TTG​TTA​AGC​GTA​GTC​TGG​GAC​GTC​GTA​TGG​GTA​CCT​CGC​CGA​GGC​GGC​TCT​GTG 
pLPCX-Fam20C-D478A-HA D478A: CAT​CAT​CCA​CTT​AGC​CAA​TGG​AAG​AGG  
pLPCX-mCherry-Fam20C Fragment1: CCA​TAA​AGC​TTA​TAC​GAA​TTC​ATA​GCC​ATG​GCT​ACA​GGC​TCC​CGG​ACG​TCC​CTG​CTC​CTG​GCT​TTT​GGC​CTG​CTC​TGC​CTG​CCC​TGG​CTT​CAA​GAG​GGC​AGT​GCC​TTC​CCA​ACC​ATT​CCC​TTA​TCC​TCG​GGA​A Fragment1: TTC​CCG​AGG​ATA​AGG​GAA​TGG​TTG​GGA​AGG​CAC​TGC​CCT​CTT​GAA​GCC​AGG​GCA​GGC​AGA​GCA​GGC​CAA​AAG​CCA​GGA​GCA​GGG​ACG​TCC​GGG​AGC​CTG​TAG​CCA​TGG 
Fragment2: CCC​TTA​TCC​TCG​GGA​ACA​AAG​CTT​ATG​GTG​AGC​AAG​GGC​GAG​G Fragment2: CCT​CCC​CGG​GGG​AGA​GGA​ATT​CCT​TAG​CGT​AGT​CTG​GGA​CGT​CGT​ATG​GGT​A 
Fragment3: TAC​CCA​TAC​GAC​GTC​CCA​GAC​TAC​GCT​AAG​GAA​TTC​CTC​TCC​CCC​GGG​GAG​G Fragment3: TAT​CGA​TGT​TTG​GCC​GAG​GCG​GCC​GCT​TAC​CTC​GCC​GAG​GCG​GCT​CTG​TG 
pIRESneo3-Str-KDEL_SS-SBP-tagRFP-Cab45 Fragment1: CTT​GCC​ACA​ACC​CGG​GAG​GCG​CGC​CAT​GGC​TAC​AGG​CTC​CCG​GAC​GTC​CC Fragment1: CTT​AAT​CAG​CTC​TTC​GCC​CTT​AGA​CAC​ACC​TGC​AGG​TGG​TTC​ACG​TTG​ACC​TTG 
Fragment2: CAA​GGT​CAA​CGT​GAA​CCA​CCT​GCA​GGT​GTG​TCT​AAG​GGC​GAA​GAG​CTG​ATT​AAG Fragment2: AGT​TAA​TTA​ATT​GGC​CCT​CGA​GGC​CTT​AAA​ACT​CCT​CGT​GCA​CGC​TGC​GC 
ConstructForward primer (5′ to 3′)Reverse primer (5′ to 3′)
pLPCX-Cab45-5pXA T131: GGA​TCA​TGG​AGA​AGG​CGG​CCG​AGC​ACT​TC S99: AGC​TTC​CTC​CGG​GCC​CGC​CGC​GGC​TC 
T142: GAG​GCC​ATG​GAG​GAG​GCC​AAG​ACA​CAC​TTC​CG 
S193: TCA​AAG​TGG​ATG​AGG​AAG​CAC​AGG​AAG​TCC​TGG​A 
S349: CGA​GTT​CTT​CAC​GGG​CGC​CAA​GCT​GGT​GGA​CTA​C 
pLPCX-Cab45-5pXE T131: GGA​TCA​TGG​AGA​AGG​AAG​CCG​AGC​ACT​TC S99: AGC​TTC​CTC​CGT​TCC​CGC​CGC​GGC​TC 
T142: GAG​GCC​ATG​GAG​GAG​GAA​AAG​ACA​CAC​TTC​CG 
S193: TCA​AAG​TGG​ATG​AGG​AAG​AAC​AGG​AAG​TCC​TGG​A 
S349: CGA​GTT​CTT​CAC​GGG​CGA​AAA​GCT​GGT​GGA​CTA​C 
pB-T-PAF-Cab45-WT/5pXA/5pXE Fragment1: GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​ATG​GCT​ACA​GGC​TCC​CGG Fragment1: GGT​TGG​CAG​GCC​GGC​ATT​CAC​CTC​C 
Fragment2: GGA​GGT​GAA​TGC​CGG​CCT​GCC​AAC​C Fragment2: CCA​GCA​CAC​TGG​ATC​AGT​TAT​CTA​TGC​TTA​AAA​CTC​CTC​GTG​CAC​GCT​GCG 
pBT-PAF-GFP-Cab45-WT/5pXA/5pXE Fragment1: GGC​GGC​CAT​CAC​AAG​TTT​GTA​CAG​CTA​GCA​TGG​CTA​CAG​GCT​CCC​G Fragment1: CCG​CTT​CCA​CCT​CCA​CCA​GAT​CTG​TGA​TGA​TGA​TGG​TGA​TGA​AGC​TTT​GTT​C 
Fragment2: CTG​GTG​GAG​GTG​GAA​GCG​GTA​GCA​AAG​GAG​AAG​AAC​TTT​TCA​CTG​G Fragment2: CTT​TGG​AAG​TAC​AGG​TTC​TCG​GAT​CCG​GCC​GGC​CGA​CCT​CCA​CCT​TTG​TAG​AGC​TC 
Fragment3: GAG​GTC​GGC​CGG​CCG​GAT​CCG​AGA​ACC​TGT​ACT​TCC​AAA​GTG​GCG​CGC​CAC​GGC​CTG​CCA​ACC​AC Fragment3: GGA​TCA​GTT​ATC​TAT​GCG​GCC​GCT​CTA​GAT​TAA​AAC​TCC​TCG​TGC​ACG​C 
pLPCX-SS-EGFP-Cab45-5pXA/5pXE Fragment1: CGT​GAC​CGC​CGC​CCC​GGA​ATT​CCG​GCC​TGC​CAA​CCA​CTC​G Fragment1: TTT​ATC​GAT​GTT​TGG​CCG​AGG​CGA​CCG​GTT​TAA​AAC​TCC​TCG​TGC​ACG​CTG​CG 
pLPCX-Fam20C-HA Fragment1: CGT​AGA​TCT​ATG​AAG​ATG​ATG​CTG​GTG​CGC​CG Fragment1: GCA​CAA​TTG​TTA​AGC​GTA​GTC​TGG​GAC​GTC​GTA​TGG​GTA​CCT​CGC​CGA​GGC​GGC​TCT​GTG 
pLPCX-Fam20C-D478A-HA D478A: CAT​CAT​CCA​CTT​AGC​CAA​TGG​AAG​AGG  
pLPCX-mCherry-Fam20C Fragment1: CCA​TAA​AGC​TTA​TAC​GAA​TTC​ATA​GCC​ATG​GCT​ACA​GGC​TCC​CGG​ACG​TCC​CTG​CTC​CTG​GCT​TTT​GGC​CTG​CTC​TGC​CTG​CCC​TGG​CTT​CAA​GAG​GGC​AGT​GCC​TTC​CCA​ACC​ATT​CCC​TTA​TCC​TCG​GGA​A Fragment1: TTC​CCG​AGG​ATA​AGG​GAA​TGG​TTG​GGA​AGG​CAC​TGC​CCT​CTT​GAA​GCC​AGG​GCA​GGC​AGA​GCA​GGC​CAA​AAG​CCA​GGA​GCA​GGG​ACG​TCC​GGG​AGC​CTG​TAG​CCA​TGG 
Fragment2: CCC​TTA​TCC​TCG​GGA​ACA​AAG​CTT​ATG​GTG​AGC​AAG​GGC​GAG​G Fragment2: CCT​CCC​CGG​GGG​AGA​GGA​ATT​CCT​TAG​CGT​AGT​CTG​GGA​CGT​CGT​ATG​GGT​A 
Fragment3: TAC​CCA​TAC​GAC​GTC​CCA​GAC​TAC​GCT​AAG​GAA​TTC​CTC​TCC​CCC​GGG​GAG​G Fragment3: TAT​CGA​TGT​TTG​GCC​GAG​GCG​GCC​GCT​TAC​CTC​GCC​GAG​GCG​GCT​CTG​TG 
pIRESneo3-Str-KDEL_SS-SBP-tagRFP-Cab45 Fragment1: CTT​GCC​ACA​ACC​CGG​GAG​GCG​CGC​CAT​GGC​TAC​AGG​CTC​CCG​GAC​GTC​CC Fragment1: CTT​AAT​CAG​CTC​TTC​GCC​CTT​AGA​CAC​ACC​TGC​AGG​TGG​TTC​ACG​TTG​ACC​TTG 
Fragment2: CAA​GGT​CAA​CGT​GAA​CCA​CCT​GCA​GGT​GTG​TCT​AAG​GGC​GAA​GAG​CTG​ATT​AAG Fragment2: AGT​TAA​TTA​ATT​GGC​CCT​CGA​GGC​CTT​AAA​ACT​CCT​CGT​GCA​CGC​TGC​GC 
View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal