Morphometric parameters of Golgi proteins in an average ministack
| Name . | GLIM . | Side-averaging . | ||||||
|---|---|---|---|---|---|---|---|---|
| LQ . | SEM . | n . | LQside . | n . | Axial position (nm) . | Normalized lateral size . | Lateral size (nm) . | |
| mGFP-sec16a | −0.81 | 0.12 | 79 | 0.04 | 39 | 12 | 0.80 | 563 |
| sec31a-GFP | −0.65a | 0.06 | 67 | −0.43 | 22 | −118 | 1.16 | 810 |
| sec23a-mCherry | −0.58b | 0.06 | 121 | −0.30 | 37 | −82 | 0.98 | 690 |
| GS27 | −0.22a | 0.03 | 101 | −0.06 | 27 | −17 | 0.71 | 500 |
| γ1COP | −0.17b | 0.07 | 106 | 0.18 | 26 | 48 | 1.10 | 770 |
| GFP-ERGIC53 | −0.16a | 0.02 | 198 | −0.05 | 24 | −12 | 0.90 | 630 |
| GM130 | 0.00c | 0.00 | 223 | 0 | 0.64 | 450 | ||
| βCOP | 0.15 | 0.06 | 183 | 0.45 | 29 | 122 | 1.11 | 784 |
| GFP-Rab1a | 0.21b | 0.03 | 154 | 0.43 | 39 | 119 | 0.57 | 400 |
| GFP-ACBD3 | 0.25b | 0.03 | 132 | 0.51 | 32 | 139 | 0.72 | 506 |
| VIP36-GFP | 0.30 | 0.02 | 134 | 0.26 | 17 | 71 | 0.64 | 450 |
| GALNT8-GFP | 0.34 | 0.05 | 73 | 0.28 | 21 | 78 | 0.61 | 430 |
| B3GALT6-Myc | 0.47b | 0.03 | 97 | 0.33 | 16 | 90 | 0.61 | 430 |
| B4GALT7-Myc | 0.52b | 0.04 | 110 | 0.14 | 19 | 39 | 0.64 | 450 |
| MGAT2-Myc | 0.53b | 0.04 | 136 | 0.50 | 34 | 138 | 0.55 | 380 |
| Giantin | 0.57b | 0.05 | 103 | 0.58 | 73 | 160 | 1.00 | 700 |
| GALNT4-GFP | 0.58 | 0.02 | 163 | 0.74 | 38 | 203 | 0.60 | 420 |
| TPST2-GFP | 0.64b | 0.02 | 154 | 0.66 | 47 | 180 | 0.54 | 380 |
| POMGNT1-Myc | 0.67b | 0.04 | 87 | 0.76 | 30 | 209 | 0.61 | 430 |
| GCNT1-GFP | 0.69 | 0.03 | 167 | 0.83 | 25 | 226 | 0.75 | 520 |
| B4GALT3-Myc | 0.74b | 0.02 | 149 | 0.78 | 42 | 215 | 0.59 | 410 |
| TPST1-GFP | 0.76a | 0.04 | 111 | 0.95 | 30 | 260 | 0.58 | 400 |
| GPP130-GFP | 0.84a | 0.02 | 168 | 0.65 | 31 | 178 | 0.90 | 630 |
| SLC35C1-Myc | 0.84b | 0.04 | 85 | 0.95 | 18 | 259 | 0.68 | 479 |
| ST6GAL1-GFP | 0.85b | 0.02 | 138 | 0.71 | 35 | 195 | 0.67 | 470 |
| GalT-mCherry | 1.00b | 1.00 | 223 | 274 | 0.67 | 470 | ||
| GFP-Rab6 | 1.04a | 0.04 | 262 | 0.95 | 26 | 260 | 0.76 | 530 |
| GFP-GOLPH3 | 1.05 | 0.03 | 99 | 0.80 | 20 | 220 | 0.87 | 610 |
| GFP-GGA1 | 1.30a | 0.12 | 33 | 1.54 | 18 | 423 | 1.75 | 1,220 |
| GFP-golgin-97 | 1.45b | 0.03 | 161 | 1.33 | 26 | 364 | 0.63 | 440 |
| CI-M6PR | 1.46b | 0.24 | 42 | 1.47 | 27 | 403 | 1.35 | 940 |
| Cab45-Myc | 1.49 | 0.07 | 96 | 1.13 | 22 | 308 | 0.76 | 530 |
| Vamp4-GFP | 1.57a | 0.04 | 157 | 1.47 | 16 | 402 | 0.66 | 470 |
| Furin-GFP | 1.64 | 0.08 | 55 | 1.69 | 28 | 463 | 0.64 | 450 |
| CLCB | 1.65b | 0.26 | 37 | 1.66 | 41 | 455 | 1.66 | 1,160 |
| SMS1-Myc | 1.76 | 0.08 | 105 | 1.51 | 28 | 414 | 0.59 | 410 |
| GGA2 | 1.96a | 0.23 | 33 | 1.48 | 20 | 405 | 1.35 | 950 |
| γ-Adaptin | 2.20 | 0.14 | 43 | 1.60 | 18 | 438 | 1.62 | 1,140 |
| Name . | GLIM . | Side-averaging . | ||||||
|---|---|---|---|---|---|---|---|---|
| LQ . | SEM . | n . | LQside . | n . | Axial position (nm) . | Normalized lateral size . | Lateral size (nm) . | |
| mGFP-sec16a | −0.81 | 0.12 | 79 | 0.04 | 39 | 12 | 0.80 | 563 |
| sec31a-GFP | −0.65a | 0.06 | 67 | −0.43 | 22 | −118 | 1.16 | 810 |
| sec23a-mCherry | −0.58b | 0.06 | 121 | −0.30 | 37 | −82 | 0.98 | 690 |
| GS27 | −0.22a | 0.03 | 101 | −0.06 | 27 | −17 | 0.71 | 500 |
| γ1COP | −0.17b | 0.07 | 106 | 0.18 | 26 | 48 | 1.10 | 770 |
| GFP-ERGIC53 | −0.16a | 0.02 | 198 | −0.05 | 24 | −12 | 0.90 | 630 |
| GM130 | 0.00c | 0.00 | 223 | 0 | 0.64 | 450 | ||
| βCOP | 0.15 | 0.06 | 183 | 0.45 | 29 | 122 | 1.11 | 784 |
| GFP-Rab1a | 0.21b | 0.03 | 154 | 0.43 | 39 | 119 | 0.57 | 400 |
| GFP-ACBD3 | 0.25b | 0.03 | 132 | 0.51 | 32 | 139 | 0.72 | 506 |
| VIP36-GFP | 0.30 | 0.02 | 134 | 0.26 | 17 | 71 | 0.64 | 450 |
| GALNT8-GFP | 0.34 | 0.05 | 73 | 0.28 | 21 | 78 | 0.61 | 430 |
| B3GALT6-Myc | 0.47b | 0.03 | 97 | 0.33 | 16 | 90 | 0.61 | 430 |
| B4GALT7-Myc | 0.52b | 0.04 | 110 | 0.14 | 19 | 39 | 0.64 | 450 |
| MGAT2-Myc | 0.53b | 0.04 | 136 | 0.50 | 34 | 138 | 0.55 | 380 |
| Giantin | 0.57b | 0.05 | 103 | 0.58 | 73 | 160 | 1.00 | 700 |
| GALNT4-GFP | 0.58 | 0.02 | 163 | 0.74 | 38 | 203 | 0.60 | 420 |
| TPST2-GFP | 0.64b | 0.02 | 154 | 0.66 | 47 | 180 | 0.54 | 380 |
| POMGNT1-Myc | 0.67b | 0.04 | 87 | 0.76 | 30 | 209 | 0.61 | 430 |
| GCNT1-GFP | 0.69 | 0.03 | 167 | 0.83 | 25 | 226 | 0.75 | 520 |
| B4GALT3-Myc | 0.74b | 0.02 | 149 | 0.78 | 42 | 215 | 0.59 | 410 |
| TPST1-GFP | 0.76a | 0.04 | 111 | 0.95 | 30 | 260 | 0.58 | 400 |
| GPP130-GFP | 0.84a | 0.02 | 168 | 0.65 | 31 | 178 | 0.90 | 630 |
| SLC35C1-Myc | 0.84b | 0.04 | 85 | 0.95 | 18 | 259 | 0.68 | 479 |
| ST6GAL1-GFP | 0.85b | 0.02 | 138 | 0.71 | 35 | 195 | 0.67 | 470 |
| GalT-mCherry | 1.00b | 1.00 | 223 | 274 | 0.67 | 470 | ||
| GFP-Rab6 | 1.04a | 0.04 | 262 | 0.95 | 26 | 260 | 0.76 | 530 |
| GFP-GOLPH3 | 1.05 | 0.03 | 99 | 0.80 | 20 | 220 | 0.87 | 610 |
| GFP-GGA1 | 1.30a | 0.12 | 33 | 1.54 | 18 | 423 | 1.75 | 1,220 |
| GFP-golgin-97 | 1.45b | 0.03 | 161 | 1.33 | 26 | 364 | 0.63 | 440 |
| CI-M6PR | 1.46b | 0.24 | 42 | 1.47 | 27 | 403 | 1.35 | 940 |
| Cab45-Myc | 1.49 | 0.07 | 96 | 1.13 | 22 | 308 | 0.76 | 530 |
| Vamp4-GFP | 1.57a | 0.04 | 157 | 1.47 | 16 | 402 | 0.66 | 470 |
| Furin-GFP | 1.64 | 0.08 | 55 | 1.69 | 28 | 463 | 0.64 | 450 |
| CLCB | 1.65b | 0.26 | 37 | 1.66 | 41 | 455 | 1.66 | 1,160 |
| SMS1-Myc | 1.76 | 0.08 | 105 | 1.51 | 28 | 414 | 0.59 | 410 |
| GGA2 | 1.96a | 0.23 | 33 | 1.48 | 20 | 405 | 1.35 | 950 |
| γ-Adaptin | 2.20 | 0.14 | 43 | 1.60 | 18 | 438 | 1.62 | 1,140 |