Table 1.
Summary of microtubule dynamics and microtubule interactions at different concentrations of HIS-TAN1
Plus-end dynamics0 µM HIS-TAN10.1 µM HIS-TAN10.5 µM HIS-TAN11 µM HIS-TAN12 µM HIS-TAN1
Growth events (n) 156 180 166 214 196 
Growth rate (μm/s, mean ± SD) 1.8 ± 0.4 1.8 ± 0.3 1.8 ± 0.3 1.7 ± 0.5* 1.5 ± 0.3*** 
Shrinkage events (n) 109 127 113 153 149 
Shrinkage rate (μm/s, mean ± SD) 31.5 ± 15.6 27.7 ± 10.8 26.2 ± 8.8* 27.8 ± 9.7 24.2 ± 10.0*** 
Time growing (%) 94.9 93.8 94.5 94.6 95.2 
Time shrinking (%) 5.1 6.2** 5.5* 5.4 4.8 
Catastrophe frequency (events/min) 0.3 0.4 0.4 0.4 0.5 
Crossovers (n) 445 346 334 334 506 
Bundling events (n) 2 0 0 3 139 
Bundling frequency (%) 0.5 0 0 0.9 27.5 
Plus-end dynamics0 µM HIS-TAN10.1 µM HIS-TAN10.5 µM HIS-TAN11 µM HIS-TAN12 µM HIS-TAN1
Growth events (n) 156 180 166 214 196 
Growth rate (μm/s, mean ± SD) 1.8 ± 0.4 1.8 ± 0.3 1.8 ± 0.3 1.7 ± 0.5* 1.5 ± 0.3*** 
Shrinkage events (n) 109 127 113 153 149 
Shrinkage rate (μm/s, mean ± SD) 31.5 ± 15.6 27.7 ± 10.8 26.2 ± 8.8* 27.8 ± 9.7 24.2 ± 10.0*** 
Time growing (%) 94.9 93.8 94.5 94.6 95.2 
Time shrinking (%) 5.1 6.2** 5.5* 5.4 4.8 
Catastrophe frequency (events/min) 0.3 0.4 0.4 0.4 0.5 
Crossovers (n) 445 346 334 334 506 
Bundling events (n) 2 0 0 3 139 
Bundling frequency (%) 0.5 0 0 0.9 27.5 

Bundling includes both zippering and pulling. Significance was calculated by comparing values to 0 µM HIS-TAN1 determined by Mann–Whitney test (*, P > 0.05; **, P > 0.01; ***, P > 0.001). Three trials were performed for each concentration of HIS-TAN1.

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