Table 1.
Primers used
PrimerSequence (5′ to 3′)
GFPBamHIF2 CGGGA​TCCGAT​GGT​GAG​CAA​AGG​CGA​AGA​G 
YPetSacIR2 CTA​GTCGAG​CTCTTA​TAC​AGT​TCG​TTC​ATG​CC 
GFPBamHIF1 CGC​GCG​GGA​TCCA​TGGTGA​GCA​AGG​GCG​AGG​AG 
GFP(TGA)HindIII CCCAAG​CTTTCA​CTT​GTA​CAG​CTC​GTC 
BamHI–importin α-F ATA​TAA​CGCGGA​TCCGAT​GCC​GAC​CAC​AAA​TG 
Importin α–XhoI-R CTA​TAC​CCGCTC​GAGCGG​AAA​TTG​AAA​GAC​TC 
GFPSacIR1 CGCGAG​CTCGAG​ATC​TGA​GTC​C 
YPetSacIR1 CTA​GTCGAG​CTCGCT​TAT​ACA​GTT​CGT​TCA​TGC​C 
PrimerSequence (5′ to 3′)
GFPBamHIF2 CGGGA​TCCGAT​GGT​GAG​CAA​AGG​CGA​AGA​G 
YPetSacIR2 CTA​GTCGAG​CTCTTA​TAC​AGT​TCG​TTC​ATG​CC 
GFPBamHIF1 CGC​GCG​GGA​TCCA​TGGTGA​GCA​AGG​GCG​AGG​AG 
GFP(TGA)HindIII CCCAAG​CTTTCA​CTT​GTA​CAG​CTC​GTC 
BamHI–importin α-F ATA​TAA​CGCGGA​TCCGAT​GCC​GAC​CAC​AAA​TG 
Importin α–XhoI-R CTA​TAC​CCGCTC​GAGCGG​AAA​TTG​AAA​GAC​TC 
GFPSacIR1 CGCGAG​CTCGAG​ATC​TGA​GTC​C 
YPetSacIR1 CTA​GTCGAG​CTCGCT​TAT​ACA​GTT​CGT​TCA​TGC​C 

Restriction sites are in bold.

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