Table 4.
Primers to create Gibson fragments
NameDescriptionSequence
AGO1963 S637X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1964 S637X_5' R gca​aag​ctg​cAG​CAG​CAT​TTT​TAG​GGC​CGT​TG 
AGO1965 S637X_3' F ggg​tcc​caa​cAG​CAG​AAG​AGG​TGG​TGC​TAC​C 
AGO1966 S637X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1967 S289X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1968 S289X_5' R ctg​gaa​cgg​tAT​CTG​CTG​TTG​CGT​GAG​CTC​G 
AGO1969 S289X_3' F ctt​cca​gca​tAA​GTG​GTT​TGA​CGA​CTT​CGC 
AGO1970 S289X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1971 S283X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1972 S283X_5' R tcg​tca​acc​aCT​TCA​ACG​TAG​GTT​TTG​AAC​GGA​AAG 
AGO1973 S283X_3' F ggt​aat​gga​aCC​GGC​ATT​CCT​TCC​AGC​ATA​AG 
AGO1974 S283X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1975 S111X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1976 S111X_5' R ctt​gcg​att​cCG​CCC​CCA​TCG​CTA​CCA​C 
AGO1977 S111X_3' F cgt​gtt​atc​tAT​CGA​GTT​GTC​GAG​CTT​CC 
AGO1978 S111X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1979 cdkmutant_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1980 cdkmutant_5' R aac​cac​ttg​aGT​CAG​GCT​TGC​GTG​CGT​TC 
AGO1981 cdkmutant_3' F acc​gca​tca​gGC​GCC​AGC​GCA​GAC​GCA​G 
AGO1982 cdkmutant_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​CGC 
NameDescriptionSequence
AGO1963 S637X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1964 S637X_5' R gca​aag​ctg​cAG​CAG​CAT​TTT​TAG​GGC​CGT​TG 
AGO1965 S637X_3' F ggg​tcc​caa​cAG​CAG​AAG​AGG​TGG​TGC​TAC​C 
AGO1966 S637X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1967 S289X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1968 S289X_5' R ctg​gaa​cgg​tAT​CTG​CTG​TTG​CGT​GAG​CTC​G 
AGO1969 S289X_3' F ctt​cca​gca​tAA​GTG​GTT​TGA​CGA​CTT​CGC 
AGO1970 S289X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1971 S283X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1972 S283X_5' R tcg​tca​acc​aCT​TCA​ACG​TAG​GTT​TTG​AAC​GGA​AAG 
AGO1973 S283X_3' F ggt​aat​gga​aCC​GGC​ATT​CCT​TCC​AGC​ATA​AG 
AGO1974 S283X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1975 S111X_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1976 S111X_5' R ctt​gcg​att​cCG​CCC​CCA​TCG​CTA​CCA​C 
AGO1977 S111X_3' F cgt​gtt​atc​tAT​CGA​GTT​GTC​GAG​CTT​CC 
AGO1978 S111X_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​C 
AGO1979 cdkmutant_5' F ccc​ttt​cgt​cTC​GCG​CGT​TTC​GGT​GAT​G 
AGO1980 cdkmutant_5' R aac​cac​ttg​aGT​CAG​GCT​TGC​GTG​CGT​TC 
AGO1981 cdkmutant_3' F acc​gca​tca​gGC​GCC​AGC​GCA​GAC​GCA​G 
AGO1982 cdkmutant_3' R aaa​cgc​gcg​aGA​CGA​AAG​GGC​CTC​GTG​ATA​CGC 
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