| . | . | Asynchronous 1 . | Arrested 1 . | Heat Stress 1 . | Asynchronous 2 . | Arrested 2 . | Heat Stress 2 . |
|---|---|---|---|---|---|---|---|
| % Coverage | 51.30% | 52.30% | 7.10% | 70.90% | 73.90% | 75.70% | |
| S025 | C | ? | NC | C | NC | C | |
| S026 | X | X | NC | ? | NC | X | |
| T034 | Cdk-1 | X | X | NC | C | C | C |
| S039 | Cdk-2 | C | X | NC | C | C | X |
| S111 | Cdk-3 | X | X | NC | X | X | X |
| S198 | GIN4-3 | X | NC | NC | C | C | C |
| S249 | Cdk-4 | X | X | NC | X | X | X |
| S283 | GIN4-4 | X | X | X | X | C | X |
| S289 | C | X | NC | C | X | C | |
| S292 | ? | ? | NC | X | C | X | |
| T294 | YAK1-1 | X | C | NC | ? | C | C |
| T313 | ? | ? | C | C | C | X | |
| T314 | Cdk-6 (full) | ? | ? | C | ? | ? | ? |
| S355 | X | X | NC | X | X | X | |
| T359 | Cdk-7, HOG1-1 | X | X | NC | C | C | C |
| S372 | GIN4-5, PKA-1 | ? | ? | NC | C | C | X |
| S373 | GIN4-6 | NC | C | NC | C | C | ? |
| S396 | NC | NC | NC | ? | ? | C | |
| S413 | ? | X | X | C | NC | C | |
| S415 | C | C | C | NC | C | ? | |
| S559 | NC | X | NC | NC | C | X | |
| S561 | ? | C | NC | NC | C | C | |
| S583 | NC | X | NC | X | C | ? | |
| Coverage of predicted kinase sites not found by mass spectrometry | |||||||
| Asynchronous 1 | Arrested 1 | Heat Stress 1 | Asynchronous 2 | Arrested 2 | Heat Stress 2 | ||
| S024 | GIN4-1 | C | C | NC | C | C | C |
| T183 | GIN4-2 | NC | NC | NC | C | C | C |
| S271 | Cdk-5 | C | C | NC | C | C | C |
| S373 | GIN4-6 | C | C | NC | C | C | ? |
| T420 | Cdk-8 | NC | C | NC | C | C | C |
| S556 | GIN4-7 | NC | C | NC | NC | NC | C |
| S637 | GIN4-8, PKA-2 | NC | NC | NC | NC | NC | NC |
| . | . | Asynchronous 1 . | Arrested 1 . | Heat Stress 1 . | Asynchronous 2 . | Arrested 2 . | Heat Stress 2 . |
|---|---|---|---|---|---|---|---|
| % Coverage | 51.30% | 52.30% | 7.10% | 70.90% | 73.90% | 75.70% | |
| S025 | C | ? | NC | C | NC | C | |
| S026 | X | X | NC | ? | NC | X | |
| T034 | Cdk-1 | X | X | NC | C | C | C |
| S039 | Cdk-2 | C | X | NC | C | C | X |
| S111 | Cdk-3 | X | X | NC | X | X | X |
| S198 | GIN4-3 | X | NC | NC | C | C | C |
| S249 | Cdk-4 | X | X | NC | X | X | X |
| S283 | GIN4-4 | X | X | X | X | C | X |
| S289 | C | X | NC | C | X | C | |
| S292 | ? | ? | NC | X | C | X | |
| T294 | YAK1-1 | X | C | NC | ? | C | C |
| T313 | ? | ? | C | C | C | X | |
| T314 | Cdk-6 (full) | ? | ? | C | ? | ? | ? |
| S355 | X | X | NC | X | X | X | |
| T359 | Cdk-7, HOG1-1 | X | X | NC | C | C | C |
| S372 | GIN4-5, PKA-1 | ? | ? | NC | C | C | X |
| S373 | GIN4-6 | NC | C | NC | C | C | ? |
| S396 | NC | NC | NC | ? | ? | C | |
| S413 | ? | X | X | C | NC | C | |
| S415 | C | C | C | NC | C | ? | |
| S559 | NC | X | NC | NC | C | X | |
| S561 | ? | C | NC | NC | C | C | |
| S583 | NC | X | NC | X | C | ? | |
| Coverage of predicted kinase sites not found by mass spectrometry | |||||||
| Asynchronous 1 | Arrested 1 | Heat Stress 1 | Asynchronous 2 | Arrested 2 | Heat Stress 2 | ||
| S024 | GIN4-1 | C | C | NC | C | C | C |
| T183 | GIN4-2 | NC | NC | NC | C | C | C |
| S271 | Cdk-5 | C | C | NC | C | C | C |
| S373 | GIN4-6 | C | C | NC | C | C | ? |
| T420 | Cdk-8 | NC | C | NC | C | C | C |
| S556 | GIN4-7 | NC | C | NC | NC | NC | C |
| S637 | GIN4-8, PKA-2 | NC | NC | NC | NC | NC | NC |
?, phosphorylated with low confidence, <50%. Blue X, phosphorylated with medium confidence, 50–90%. Green X, phosphorylated with high confidence, >90%. Red X, phosphorylated with high confidence with a lot more hits than in asynchronous. C, region covered by mass spectrometry. NC, region not covered by mass spectrometry.