Table 1.
Sites phosphorylated with any confidence
  Asynchronous 1Arrested 1Heat Stress 1Asynchronous 2Arrested 2Heat Stress 2
% Coverage   51.30% 52.30% 7.10% 70.90% 73.90% 75.70% 
S025   C ? NC C NC C 
S026   X X NC ? NC X 
T034 Cdk-1 X X NC C C C 
S039 Cdk-2 C X NC C C X 
S111 Cdk-3 X X NC X X X 
S198 GIN4-3 X NC NC C C C 
S249 Cdk-4 X X NC X X X 
S283 GIN4-4 X X X X C X 
S289   C X NC C X C 
S292   ? ? NC X C X 
T294 YAK1-1 X C NC ? C C 
T313   ? ? C C C X 
T314 Cdk-6 (full) ? ? C ? ? ? 
S355   X X NC X X X 
T359 Cdk-7, HOG1-1 X X NC C C C 
S372 GIN4-5, PKA-1 ? ? NC C C X 
S373 GIN4-6 NC C NC C C ? 
S396   NC NC NC ? ? C 
S413   ? X X C NC C 
S415   C C C NC C ? 
S559   NC X NC NC C X 
S561   ? C NC NC C C 
S583   NC X NC X C ? 
Coverage of predicted kinase sites not found by mass spectrometry 
  Asynchronous 1 Arrested 1 Heat Stress 1 Asynchronous 2 Arrested 2 Heat Stress 2 
S024 GIN4-1 C C NC C C C 
T183 GIN4-2 NC NC NC C C C 
S271 Cdk-5 C C NC C C C 
S373 GIN4-6 C C NC C C ? 
T420 Cdk-8 NC C NC C C C 
S556 GIN4-7 NC C NC NC NC C 
S637 GIN4-8, PKA-2 NC NC NC NC NC NC 

?, phosphorylated with low confidence, <50%. Blue X, phosphorylated with medium confidence, 50–90%. Green X, phosphorylated with high confidence, >90%. Red X, phosphorylated with high confidence with a lot more hits than in asynchronous. C, region covered by mass spectrometry. NC, region not covered by mass spectrometry.

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