Table 2. Synthesized maternal genotypes... | Rockefeller University Press

Table 2.

Synthesized maternal genotypes used in this study

Par-1 RNAi rescue experiments
mgv/+; UASp-mCherry(mCh)-shRNA/+
mgv/+; UASp-Par-1-shRNA/+
mgv/+; UASp-GFP-Par-1 (RNAi resistant)/+; UASp-Par-1-shRNA/+
mgv/+; UASp-Par-1KD-GFP (RNAi resistant)/+; UASp-Par-1-shRNA/+
Analyses of known Par-1 phosphorylation targets
mgv/+; Zip-GFP trap/+; UASp-mCh-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-Mbs/+
mgv/+; Zip-GFP trap/+; UASp-Mbs^T506A/+
mgv/+; UASp-GFP/+
mgv/+; UASp-Baz-GFP/+
mgv/+; UASp-Baz^S151AS1085A-GFP/+
mgv/+; UASp-mCh-shRNA/+
mgv/+; UASp-Dlg-shRNA/+
mgv/+; UASp-Dlg-shRNA/UASp-mCh-shRNA
mgv/+; UASp-Dlg-shRNA/UASp-Par-1-shRNA
Par-1 localization analyses
mgv/+; UASp-GFP-Par-1 (RNAi resistant)/+; UASp-Par-1-shRNA/+
mgv/+; Par-1-GFP trap/+; UASp-mCh-shRNA
mgv/+; Par-1-GFP trap/+; UASp-Dia-shRNA (35479)
Live imaging of cytoskeleton markers
mgv/+; sqh-Moe-ABD-GFP/+; UASp-mCh-shRNA/+
mgv/+; sqh-Moe-ABD-GFP/+; UASp-Par-1-shRNA/+
mgv/+; sqh-Moe-ABD-GFP/UASp-Dia-shRNA (35479)
mgv/+; sqh-Moe-ABD-GFP/UASp-Arp3-shRNA
mgv/+; sqh-Moe-ABD-GFP/+; UASp-Zip-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-mCh-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-Par-1-shRNA/+
mgv/+; Zip-GFP trap/UAS-Dia-shRNA (35479)
mgv; UASp-mCherry-Tubulin
mgv/+; UASp-mCherry-Tubulin/UASp-Par-1-shRNA
mgv/+; ncd-ncd-mRFP/UASp-Par-1-shRNA
Dia, Par-1, Arp3, and F-actin fixed localization studies
mgv/+; UASp-mCh-shRNA/+
mgv/+; UASp-Par-1-shRNA/+
mgv/+; UASp-Dia-shRNA (35479)/+
mgv/+; UASp-Arp3-shRNA/+
mgv/+; UASp-GFP-Par-1/+
mgv/+; UASp-Arp3-GFP/+
mgv/+; UASp-GFP-Par-1/UASp-Arp3-shRNA
Dia RNAi enhancement experiments
mgv/+; par-1^w3/sqh-Moe-ABD-GFP
mgv/+; par-1^w3/sqh-Moe-ABD-GFP; UASp-Dia-shRNA (33424)/+
mgv/+; Cyo/sqh-Moe-ABD-GFP; UASp-Dia-shRNA (33424)/+ (sibling of line above)
Dia RNAi suppression experiments
mgv/+; UASp-GFP/UASp-Dia-shRNA (33424)
mgv/+; UASp-GFP-Par-1 (RNAi resistant)/+; UASp-Dia-shRNA (33424)/+
mgv/+; UASp-Par-1KD-GFP (RNAi resistant)/+; UASp-Dia-shRNA (33424)/+
RhoGEF2-Rho1 pathway experiments
mgv/+; Zip-GFP trap/+; UASp-mCh-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-Par-1-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-RhoGEF2-shRNA (34643)/+
mgv/+; Zip-GFP trap/+; UASp-Rok-shRNA/+
mgv/+; Anillin-RBD-GFP/UASp-mCh-shRNA
mgv/+; Anillin-RBD-GFP/UASp-Par-1-shRNA
mgv/+; Anillin-RBD-GFP/UASp-RhoGEF2-shRNA (34643)

Par-1 RNAi rescue experiments
mgv/+; UASp-mCherry(mCh)-shRNA/+
mgv/+; UASp-Par-1-shRNA/+
mgv/+; UASp-GFP-Par-1 (RNAi resistant)/+; UASp-Par-1-shRNA/+
mgv/+; UASp-Par-1KD-GFP (RNAi resistant)/+; UASp-Par-1-shRNA/+
Analyses of known Par-1 phosphorylation targets
mgv/+; Zip-GFP trap/+; UASp-mCh-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-Mbs/+
mgv/+; Zip-GFP trap/+; UASp-Mbs^T506A/+
mgv/+; UASp-GFP/+
mgv/+; UASp-Baz-GFP/+
mgv/+; UASp-Baz^S151AS1085A-GFP/+
mgv/+; UASp-mCh-shRNA/+
mgv/+; UASp-Dlg-shRNA/+
mgv/+; UASp-Dlg-shRNA/UASp-mCh-shRNA
mgv/+; UASp-Dlg-shRNA/UASp-Par-1-shRNA
Par-1 localization analyses
mgv/+; UASp-GFP-Par-1 (RNAi resistant)/+; UASp-Par-1-shRNA/+
mgv/+; Par-1-GFP trap/+; UASp-mCh-shRNA
mgv/+; Par-1-GFP trap/+; UASp-Dia-shRNA (35479)
Live imaging of cytoskeleton markers
mgv/+; sqh-Moe-ABD-GFP/+; UASp-mCh-shRNA/+
mgv/+; sqh-Moe-ABD-GFP/+; UASp-Par-1-shRNA/+
mgv/+; sqh-Moe-ABD-GFP/UASp-Dia-shRNA (35479)
mgv/+; sqh-Moe-ABD-GFP/UASp-Arp3-shRNA
mgv/+; sqh-Moe-ABD-GFP/+; UASp-Zip-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-mCh-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-Par-1-shRNA/+
mgv/+; Zip-GFP trap/UAS-Dia-shRNA (35479)
mgv; UASp-mCherry-Tubulin
mgv/+; UASp-mCherry-Tubulin/UASp-Par-1-shRNA
mgv/+; ncd-ncd-mRFP/UASp-Par-1-shRNA
Dia, Par-1, Arp3, and F-actin fixed localization studies
mgv/+; UASp-mCh-shRNA/+
mgv/+; UASp-Par-1-shRNA/+
mgv/+; UASp-Dia-shRNA (35479)/+
mgv/+; UASp-Arp3-shRNA/+
mgv/+; UASp-GFP-Par-1/+
mgv/+; UASp-Arp3-GFP/+
mgv/+; UASp-GFP-Par-1/UASp-Arp3-shRNA
Dia RNAi enhancement experiments
mgv/+; par-1^w3/sqh-Moe-ABD-GFP
mgv/+; par-1^w3/sqh-Moe-ABD-GFP; UASp-Dia-shRNA (33424)/+
mgv/+; Cyo/sqh-Moe-ABD-GFP; UASp-Dia-shRNA (33424)/+ (sibling of line above)
Dia RNAi suppression experiments
mgv/+; UASp-GFP/UASp-Dia-shRNA (33424)
mgv/+; UASp-GFP-Par-1 (RNAi resistant)/+; UASp-Dia-shRNA (33424)/+
mgv/+; UASp-Par-1KD-GFP (RNAi resistant)/+; UASp-Dia-shRNA (33424)/+
RhoGEF2-Rho1 pathway experiments
mgv/+; Zip-GFP trap/+; UASp-mCh-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-Par-1-shRNA/+
mgv/+; Zip-GFP trap/+; UASp-RhoGEF2-shRNA (34643)/+
mgv/+; Zip-GFP trap/+; UASp-Rok-shRNA/+
mgv/+; Anillin-RBD-GFP/UASp-mCh-shRNA
mgv/+; Anillin-RBD-GFP/UASp-Par-1-shRNA
mgv/+; Anillin-RBD-GFP/UASp-RhoGEF2-shRNA (34643)

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