Table 1.
Resources used in this study
ReagentSourceNumber
Antibodies   
Mouse anti–β-tubulin (1:100) Developmental Studies Hybridoma Bank (DSHB) DSHB Cat# E7, RRID:AB_528499 
Rabbit anti-Dia (1:3,500) S. Wasserman, University of California at San Diego, La Jolla, CA N/A 
Rabbit anti-Amph (1:1,000) G. Boulianne, Hospital for Sick Children, Toronto, Ontario, Canada N/A 
Mouse anti-Dlg (1:100) DSHB DSHB Cat# 4F3, RRID:AB_528203 
Alexa Fluor 568–conjugated phalloidin (1:200) Invitrogen Cat# A-12380 
Goat anti-mouse Alexa Fluor 568 (1:500) Invitrogen Cat# A-11004, RRID:AB_141371 
Goat anti-mouse Alexa Fluor 647 (1:500) Invitrogen Cat# A-21235, RRID:AB_141693 
Goat anti-rabbit Alexa Fluor 488 (1:500) Invitrogen Cat# A-11008, RRID:AB_143165 
Goat anti-rabbit Alexa Fluor 568 (1:500) Invitrogen Cat# A-11011, RRID:AB_143157 
Goat anti-rabbit Alexa Fluor 647 (1:500) Invitrogen Cat# A-21245, RRID:AB_141775 
Mounting reagents   
Aqua Polymount Polysciences Cat #18606-5 
Halocarbon oil (series 700) Halocarbon Products Cat #9002-83-9 
Drosophila lines   
Maternal-α4-tubulin-GAL4::VP16 M. Peifer, University of North Carolina at Chapel Hill, Chapel Hill, NC N/A 
UASp-mCherry-shRNA Bloomington Drosophila Stock Center (BDSC) BDSC #35785 
UASp-Par-1-shRNA BDSC BDSC #35342 
UASp-Arp3-shRNA BDSC BDSC #53972 
UASp-Dia-shRNA (33424) BDSC BDSC #33424 
UASp-Dia-shRNA (35479) BDSC BDSC #35479 
UASp-RhoGEF2-shRNA (34643) BDSC BDSC #34643 
UASp-RhoGEF2-shRNA (76255) BDSC BDSC #76255 
UASp-Rok-shRNA Zhang et al., 2018  N/A 
UASp-Dlg-shRNA BDSC BDSC #35286 
UASp-Zip-shRNA BDSC BDSC #36727 
UASp-GFP U. Tepass, University of Toronto N/A 
UASp-GFP-Par-1 This study N/A 
UASp-GFP-Par-1KD This study N/A 
UASp-Mbs This study N/A 
UASp-MbsT506A This study N/A 
UASp-Baz-GFP McKinley et al., 2012  N/A 
UASp-BazS151AS1085A-GFP Jiang et al., 2015  N/A 
UASp-Arp3-GFP BDSC BDSC #39722 
Anillin-RBD-GFP T. Lecuit, Developmental Biology Institute of Marseille, France N/A 
Par-1-GFP Trap Flytrap CC01981 
Zip-GFP Trap Flytrap #51564 
UASp-mCherry-Tubulin N. Rusan, National Heart, Lung, and Blood Institute, Bethesda, MD N/A 
ncd-ncd-mRFP BDSC BDSC #58372 
sqh-Moe-ABD-GFP D. Kiehart, Duke University, Durham Hill, NC N/A 
par-1w3/Cyo D. St Johnston, Cambridge University, Cambridge, UK N/A 
Oligonucleotides   
Primers for par-1 coding region PCR amplification: 5’-CGC​GGA​TCC​GAT​GTC​GAC​AGC​AAT​GCG​CAC​CAC​ACT​GC-3’ (forward) and 5’-CCG​CTC​GAG​TTA​CTG​GTT​TGG​CCG​TTT​GCT​AAA​ACG-3’ (reverse) Life Technologies N/A 
Primers for site-directed mutagenesis to generate Par-1–KD: 5'-CGC​GTT​CTG​CGG​TGC​CCC​GCC​ATA​T-3' (forward) and 5'-ATA​TGG​CGG​GGC​ACC​GCA​GAA​CGC​G-3' (reverse) Life Technologies N/A 
Primers for mbs coding region PCR amplification: 5’-CAA​GAA​GGA​TCT​GAA​TTC​ATG​TCC​TCG​CTG​GAC​GCA-3’ (forward) and 5’-GTG​CGA​GTT​CGC​GGC​CGC​TCA​TTT​ACT​TAA​TTT​GC-3’ (reverse) Life Technologies N/A 
Primers for site-directed mutagenesis to generate MbsT506A: 5'-CGA​CGG​TCT​GCC​CAA​GGT​GTC​ACC​CTG-3' (forward) and 5'-CAG​GGT​GAC​ACC​TTG​GGC​AGA​CCG​TCG-3' (reverse) Life Technologies N/A 
Recombinant DNA   
Drosophila Par-1 N1S cDNA clone Drosophila Genomics Resource Center (DGRC) RE47050 
Drosophila Mbs cDNA clone DGRC RE63915 
pENTR Invitrogen Cat# A-10463 
pPGW DGRC DGRC:1077 
Software and algorithms   
Volocity 4.2.1 Quorum Technologies Inc. N/A 
ImageJ National Institutes of Health  N/A 
Ridge Detection 1.4.0 ImageJ plugin N/A 
Adobe Photoshop CS4 Adobe  N/A 
ReagentSourceNumber
Antibodies   
Mouse anti–β-tubulin (1:100) Developmental Studies Hybridoma Bank (DSHB) DSHB Cat# E7, RRID:AB_528499 
Rabbit anti-Dia (1:3,500) S. Wasserman, University of California at San Diego, La Jolla, CA N/A 
Rabbit anti-Amph (1:1,000) G. Boulianne, Hospital for Sick Children, Toronto, Ontario, Canada N/A 
Mouse anti-Dlg (1:100) DSHB DSHB Cat# 4F3, RRID:AB_528203 
Alexa Fluor 568–conjugated phalloidin (1:200) Invitrogen Cat# A-12380 
Goat anti-mouse Alexa Fluor 568 (1:500) Invitrogen Cat# A-11004, RRID:AB_141371 
Goat anti-mouse Alexa Fluor 647 (1:500) Invitrogen Cat# A-21235, RRID:AB_141693 
Goat anti-rabbit Alexa Fluor 488 (1:500) Invitrogen Cat# A-11008, RRID:AB_143165 
Goat anti-rabbit Alexa Fluor 568 (1:500) Invitrogen Cat# A-11011, RRID:AB_143157 
Goat anti-rabbit Alexa Fluor 647 (1:500) Invitrogen Cat# A-21245, RRID:AB_141775 
Mounting reagents   
Aqua Polymount Polysciences Cat #18606-5 
Halocarbon oil (series 700) Halocarbon Products Cat #9002-83-9 
Drosophila lines   
Maternal-α4-tubulin-GAL4::VP16 M. Peifer, University of North Carolina at Chapel Hill, Chapel Hill, NC N/A 
UASp-mCherry-shRNA Bloomington Drosophila Stock Center (BDSC) BDSC #35785 
UASp-Par-1-shRNA BDSC BDSC #35342 
UASp-Arp3-shRNA BDSC BDSC #53972 
UASp-Dia-shRNA (33424) BDSC BDSC #33424 
UASp-Dia-shRNA (35479) BDSC BDSC #35479 
UASp-RhoGEF2-shRNA (34643) BDSC BDSC #34643 
UASp-RhoGEF2-shRNA (76255) BDSC BDSC #76255 
UASp-Rok-shRNA Zhang et al., 2018  N/A 
UASp-Dlg-shRNA BDSC BDSC #35286 
UASp-Zip-shRNA BDSC BDSC #36727 
UASp-GFP U. Tepass, University of Toronto N/A 
UASp-GFP-Par-1 This study N/A 
UASp-GFP-Par-1KD This study N/A 
UASp-Mbs This study N/A 
UASp-MbsT506A This study N/A 
UASp-Baz-GFP McKinley et al., 2012  N/A 
UASp-BazS151AS1085A-GFP Jiang et al., 2015  N/A 
UASp-Arp3-GFP BDSC BDSC #39722 
Anillin-RBD-GFP T. Lecuit, Developmental Biology Institute of Marseille, France N/A 
Par-1-GFP Trap Flytrap CC01981 
Zip-GFP Trap Flytrap #51564 
UASp-mCherry-Tubulin N. Rusan, National Heart, Lung, and Blood Institute, Bethesda, MD N/A 
ncd-ncd-mRFP BDSC BDSC #58372 
sqh-Moe-ABD-GFP D. Kiehart, Duke University, Durham Hill, NC N/A 
par-1w3/Cyo D. St Johnston, Cambridge University, Cambridge, UK N/A 
Oligonucleotides   
Primers for par-1 coding region PCR amplification: 5’-CGC​GGA​TCC​GAT​GTC​GAC​AGC​AAT​GCG​CAC​CAC​ACT​GC-3’ (forward) and 5’-CCG​CTC​GAG​TTA​CTG​GTT​TGG​CCG​TTT​GCT​AAA​ACG-3’ (reverse) Life Technologies N/A 
Primers for site-directed mutagenesis to generate Par-1–KD: 5'-CGC​GTT​CTG​CGG​TGC​CCC​GCC​ATA​T-3' (forward) and 5'-ATA​TGG​CGG​GGC​ACC​GCA​GAA​CGC​G-3' (reverse) Life Technologies N/A 
Primers for mbs coding region PCR amplification: 5’-CAA​GAA​GGA​TCT​GAA​TTC​ATG​TCC​TCG​CTG​GAC​GCA-3’ (forward) and 5’-GTG​CGA​GTT​CGC​GGC​CGC​TCA​TTT​ACT​TAA​TTT​GC-3’ (reverse) Life Technologies N/A 
Primers for site-directed mutagenesis to generate MbsT506A: 5'-CGA​CGG​TCT​GCC​CAA​GGT​GTC​ACC​CTG-3' (forward) and 5'-CAG​GGT​GAC​ACC​TTG​GGC​AGA​CCG​TCG-3' (reverse) Life Technologies N/A 
Recombinant DNA   
Drosophila Par-1 N1S cDNA clone Drosophila Genomics Resource Center (DGRC) RE47050 
Drosophila Mbs cDNA clone DGRC RE63915 
pENTR Invitrogen Cat# A-10463 
pPGW DGRC DGRC:1077 
Software and algorithms   
Volocity 4.2.1 Quorum Technologies Inc. N/A 
ImageJ National Institutes of Health  N/A 
Ridge Detection 1.4.0 ImageJ plugin N/A 
Adobe Photoshop CS4 Adobe  N/A 
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