Table 1.
Sequences of primers used
GeneForward primer, 5′-3′Reverse primer, 5′-3′
Mouse   
β-actin GGC​TGT​ATT​CCC​CTC​CAT​CG CCA​GTT​GGT​AAC​AAT​GCC​ATG​T 
eMLV spliced CCA​GGG​ACC​ACC​GAC​CCA​CCG TAG​TCG​GTC​CCG​GTA​GGC​CTC​G 
GLN TGT​GTA​AGT​CCA​GAC​GCA​G CCA​ACC​TAC​TCC​AAA​AAC​AG 
MervlPol ATC​TCC​TGG​CAC​CTG​GTA​TG AGA​AGA​AGG​CAT​TTG​CCA​GA 
MMERVK CAA​ATA​GCC​CTA​CCA​TAT​GTC​AG GTA​TAC​TTT​CTT​CTT​CAG​GTC​CAC 
MoMLV CGA​GAC​CTC​ATC​ACC​CAG​GTT​AAG CAT​CAG​AGC​AGC​CGA​TTG​TCT​G 
MTA ATG​TTT​TGG​GGA​GGA​CTG​TG AGC​CCC​AGC​TAA​CCA​GAA​C 
MusD GTG​CTA​ACC​CAA​CGC​TGG​TTC CTC​TGG​CCT​GAA​ACA​ACT​CCT​G 
pMLV CCG​CCA​GGT​CCT​CAA​TAT​AG AGAAGGTGGGGCAGTCT 
xMLV TCT​ATG​GTA​CCT​GGG​GCT​C GGC​AGA​GGT​ATG​GTT​GGA​GTA​G 
Mx1 GAC​CAT​AGG​GGT​CTT​GAC​CAA AGA​CTT​GCT​CTT​TCT​GAA​AAG​CC 
Mx2 GAG​GCT​CTT​CAG​AAT​GAG​CAA​A CTC​TGC​GGT​CAG​TCT​CTC​T 
Oas1b GGC​CTC​TAA​GGG​GGT​CAA​G CTG​GCA​GCA​CGT​CAA​ACT​TC 
Isg15 GGT​GTC​CGT​GAC​TAA​CTC​CAT TGG​AAA​GGG​TAA​GAC​CGT​CCT 
Oasl1 CAG​GAG​CTG​TAC​GGC​TTC​C CCT​ACC​TTG​AGT​ACC​TTG​AGC​AC 
Ddx58 AAG​AGC​CAG​AGT​GTC​AGA​ATC​T AGC​TCC​AGT​TGG​TAA​TTT​CTT​GG 
Ifna1 CTG​TGC​TTT​CCT​GAT​GGT​CCT TCC​ATG​CAG​CAG​ATG​AGT​CCT 
Ifnb1 AGC​TCC​AAG​AAA​GGA​CGA​ACA GCC​CTG​TAG​GTG​AGG​TTG​AT 
Ifng ATA​AGC​GTC​ATT​GAA​TCA​CAC CTC​AAA​CTT​GGC​AAT​ACT​CA 
Ifnl2 GCA​GAC​CTG​TAC​ACA​GCT​TCA CAG​GTT​GGA​GGT​GAC​AGA​GG 
Ifnl3 GTT​CAA​GTC​TCT​GTC​CCC​AAA​A GTG​GGA​ACT​GCA​CCT​CAT​GT 
Ifnar1 CTT​GTA​GCC​TCA​CCG​CCT​A CAA​CAT​CCA​CAA​GCA​CGT​CCA 
Ifngr1 GTG​GAG​CTT​TGA​CGA​GCA​CT ATT​CCC​AGC​ATA​CGA​CAG​GGT 
Il10rb ACC​TGC​TTT​CCC​CAA​AAC​GAA TGA​GAG​AAG​TCG​CAC​TGA​GTC 
Stat1 AAA​TGC​CCA​AAG​ATT​TAA​TCA​GG TTG​GTC​GCT​CTT​CGC​CAC​A 
Irf1 ATG​CCA​ATC​ACT​CGA​ATG​CG TTG​TAT​CGG​CCT​GTG​TGA​ATG 
Irf5 GGT​CAA​CGG​GGA​AAA​GAA​ACT CAT​CCA​CCC​CTT​CAG​TGT​ACT 
Irf7 CAC​AGT​CTT​CCG​CGT​ACC​C TCC​CGG​CTA​AGT​TCG​TAC​ACC 
Irf9 GAA​ATC​ACC​ATG​CAA​GCG​AAG TGA​GTG​GTT​TAC​AAC​GCC​AT 
Gbp3 GAG​GCA​CCC​ATT​TGT​CTG​GT CCG​TCC​TGC​AAG​ACG​ATT​CA 
CD274 (PD-L1) GCT​CCA​AAG​GAC​TTG​TAC​GTG TGA​TCT​GAA​GGG​CAG​CAT​TTC 
CD274-L2A TAC​AGC​TGA​ATT​GGT​CAT​CCC​A GGA​TGG​CCA​TCG​TGC​TAG​GAA 
Human   
β-actin CAT​GTA​CGT​TGC​TAT​CCA​GGC CTC​CTT​AAT​GTC​ACG​CAC​GAT 
HLINEORF1F TTG​GAA​AAC​ACT​CTG​CAG​GAT​ATT​AT TTG​GCC​TGC​CTT​GCT​AGA​TT 
HERV-W TGA​GTC​AAT​TCT​CAT​ACC​TG AGT​TAA​GAG​TTC​TTG​GGT​GG 
HERV-H TGGTGCCGTGACTCG GAT GCT​GAG​TCC​GAA​AAG​AGA​GTC 
ERVF2B AAA​AAG​GAA​GAA​GTT​AAC​AGC ATA​TAA​AGA​CTT​AGG​TCC​TGC 
HERV-E GGT​GTC​ACT​ACT​CAA​TAC​AC GCAGCCTAGGTCTCTGG 
HERV-F CCT​CCA​GTC​ACA​ACA​ACT​C TAT​TGA​AGA​AGG​CGG​CTG​G 
HERV-K AAAGAACCAGCCACCAGG CAG​TCT​GAA​AAC​TTT​TCT​CTC 
HML-5 TGAAAGGCCAGCTTGCTG CAA​TTA​GGA​AAT​TCT​TTT​CTA​C 
OASL1 CTG​ATG​CAG​GAA​CTG​TAT​AGC​AC CAC​AGC​GTC​TAG​CAC​CTC​TT 
DHX58 GGG​CCT​CCA​AAC​TCG​ATG​G TTC​TGG​GGT​GAC​ATG​ATG​CAC 
MX1 GTT​TCC​GAA​GTG​GAC​ATC​GCA CTG​CAC​AGG​TTG​TTC​TCA​GC 
ISG15 CGC​AGA​TCA​CCC​AGA​AGA​TCG TTC​GTC​GCA​TTT​GTC​CAC​CA 
OAS1 GAA​GGC​AGC​TCA​CGA​AAC​C AGGCCTCAGCCTCTTGTG 
IFIT1 TGT​TGA​AGC​AGA​AGC​ACA​CA TCT​ACG​CGA​TGT​TTC​CTA​CG 
MX2 CAG​AGG​CAG​CGG​AAT​CGT​AA TGA​AGC​TCT​AGC​TCG​GTG​TTC 
IFI44 GGT​GGG​CAC​TAA​TAC​AAC​TGG CAC​ACA​GAA​TAA​ACG​GCA​GGT​A 
OAS1 TGT​CCA​AGG​TGG​TAA​AGG​GTG CCG​GCG​ATT​TAA​CTG​ATC​CTG 
OAS2 ACG​TGA​CAT​CCT​CGA​TAA​AAC​TG GAA​CCC​ATC​AAG​GGA​CTT​CTG 
DDX60 CAG​CTC​CAA​TGA​AAT​GGT​GCC CTC​AGG​GGT​TTA​TGA​GAA​TGC​C 
IDH2 CGC​CAC​TAT​GCC​GAC​AAA​AG ACT​GCC​AGA​TAA​TAC​GGG​TCA 
STAT1 CAA​TGG​AAC​TTG​ATG​GCC​CTA TTC​TAC​AGA​GCC​CAC​TAT​CCG 
IRF1 ATG​CCC​ATC​ACT​CGG​ATG​C CCC​TGC​TTT​GTA​TCG​GCC​TG 
IRF5 GGG​CTT​CAA​TGG​GTC​AAC​G GCC​TTC​GGT​GTA​TTT​CCC​TG 
IRF7 GCT​GGA​CGT​GAC​CAT​CAT​GTA GGG​CCG​TAT​AGG​AAC​GTG​C 
IRF9 GCC​CTA​CAA​GGT​GTA​TCA​GTT​G TGC​TGT​CGC​TTT​GAT​GGT​ACT 
CD274 (PD-L1) TGG​CAT​TTG​CTG​AAC​GCA​TTT TGC​AGC​CAG​GTC​TAA​TTG​TTT​T 
CD274-L2A TAC​AGC​TGA​ATT​GGT​CAT​CCC​A AGG​CAG​ACA​TCA​TGC​TAG​GTG 
GeneForward primer, 5′-3′Reverse primer, 5′-3′
Mouse   
β-actin GGC​TGT​ATT​CCC​CTC​CAT​CG CCA​GTT​GGT​AAC​AAT​GCC​ATG​T 
eMLV spliced CCA​GGG​ACC​ACC​GAC​CCA​CCG TAG​TCG​GTC​CCG​GTA​GGC​CTC​G 
GLN TGT​GTA​AGT​CCA​GAC​GCA​G CCA​ACC​TAC​TCC​AAA​AAC​AG 
MervlPol ATC​TCC​TGG​CAC​CTG​GTA​TG AGA​AGA​AGG​CAT​TTG​CCA​GA 
MMERVK CAA​ATA​GCC​CTA​CCA​TAT​GTC​AG GTA​TAC​TTT​CTT​CTT​CAG​GTC​CAC 
MoMLV CGA​GAC​CTC​ATC​ACC​CAG​GTT​AAG CAT​CAG​AGC​AGC​CGA​TTG​TCT​G 
MTA ATG​TTT​TGG​GGA​GGA​CTG​TG AGC​CCC​AGC​TAA​CCA​GAA​C 
MusD GTG​CTA​ACC​CAA​CGC​TGG​TTC CTC​TGG​CCT​GAA​ACA​ACT​CCT​G 
pMLV CCG​CCA​GGT​CCT​CAA​TAT​AG AGAAGGTGGGGCAGTCT 
xMLV TCT​ATG​GTA​CCT​GGG​GCT​C GGC​AGA​GGT​ATG​GTT​GGA​GTA​G 
Mx1 GAC​CAT​AGG​GGT​CTT​GAC​CAA AGA​CTT​GCT​CTT​TCT​GAA​AAG​CC 
Mx2 GAG​GCT​CTT​CAG​AAT​GAG​CAA​A CTC​TGC​GGT​CAG​TCT​CTC​T 
Oas1b GGC​CTC​TAA​GGG​GGT​CAA​G CTG​GCA​GCA​CGT​CAA​ACT​TC 
Isg15 GGT​GTC​CGT​GAC​TAA​CTC​CAT TGG​AAA​GGG​TAA​GAC​CGT​CCT 
Oasl1 CAG​GAG​CTG​TAC​GGC​TTC​C CCT​ACC​TTG​AGT​ACC​TTG​AGC​AC 
Ddx58 AAG​AGC​CAG​AGT​GTC​AGA​ATC​T AGC​TCC​AGT​TGG​TAA​TTT​CTT​GG 
Ifna1 CTG​TGC​TTT​CCT​GAT​GGT​CCT TCC​ATG​CAG​CAG​ATG​AGT​CCT 
Ifnb1 AGC​TCC​AAG​AAA​GGA​CGA​ACA GCC​CTG​TAG​GTG​AGG​TTG​AT 
Ifng ATA​AGC​GTC​ATT​GAA​TCA​CAC CTC​AAA​CTT​GGC​AAT​ACT​CA 
Ifnl2 GCA​GAC​CTG​TAC​ACA​GCT​TCA CAG​GTT​GGA​GGT​GAC​AGA​GG 
Ifnl3 GTT​CAA​GTC​TCT​GTC​CCC​AAA​A GTG​GGA​ACT​GCA​CCT​CAT​GT 
Ifnar1 CTT​GTA​GCC​TCA​CCG​CCT​A CAA​CAT​CCA​CAA​GCA​CGT​CCA 
Ifngr1 GTG​GAG​CTT​TGA​CGA​GCA​CT ATT​CCC​AGC​ATA​CGA​CAG​GGT 
Il10rb ACC​TGC​TTT​CCC​CAA​AAC​GAA TGA​GAG​AAG​TCG​CAC​TGA​GTC 
Stat1 AAA​TGC​CCA​AAG​ATT​TAA​TCA​GG TTG​GTC​GCT​CTT​CGC​CAC​A 
Irf1 ATG​CCA​ATC​ACT​CGA​ATG​CG TTG​TAT​CGG​CCT​GTG​TGA​ATG 
Irf5 GGT​CAA​CGG​GGA​AAA​GAA​ACT CAT​CCA​CCC​CTT​CAG​TGT​ACT 
Irf7 CAC​AGT​CTT​CCG​CGT​ACC​C TCC​CGG​CTA​AGT​TCG​TAC​ACC 
Irf9 GAA​ATC​ACC​ATG​CAA​GCG​AAG TGA​GTG​GTT​TAC​AAC​GCC​AT 
Gbp3 GAG​GCA​CCC​ATT​TGT​CTG​GT CCG​TCC​TGC​AAG​ACG​ATT​CA 
CD274 (PD-L1) GCT​CCA​AAG​GAC​TTG​TAC​GTG TGA​TCT​GAA​GGG​CAG​CAT​TTC 
CD274-L2A TAC​AGC​TGA​ATT​GGT​CAT​CCC​A GGA​TGG​CCA​TCG​TGC​TAG​GAA 
Human   
β-actin CAT​GTA​CGT​TGC​TAT​CCA​GGC CTC​CTT​AAT​GTC​ACG​CAC​GAT 
HLINEORF1F TTG​GAA​AAC​ACT​CTG​CAG​GAT​ATT​AT TTG​GCC​TGC​CTT​GCT​AGA​TT 
HERV-W TGA​GTC​AAT​TCT​CAT​ACC​TG AGT​TAA​GAG​TTC​TTG​GGT​GG 
HERV-H TGGTGCCGTGACTCG GAT GCT​GAG​TCC​GAA​AAG​AGA​GTC 
ERVF2B AAA​AAG​GAA​GAA​GTT​AAC​AGC ATA​TAA​AGA​CTT​AGG​TCC​TGC 
HERV-E GGT​GTC​ACT​ACT​CAA​TAC​AC GCAGCCTAGGTCTCTGG 
HERV-F CCT​CCA​GTC​ACA​ACA​ACT​C TAT​TGA​AGA​AGG​CGG​CTG​G 
HERV-K AAAGAACCAGCCACCAGG CAG​TCT​GAA​AAC​TTT​TCT​CTC 
HML-5 TGAAAGGCCAGCTTGCTG CAA​TTA​GGA​AAT​TCT​TTT​CTA​C 
OASL1 CTG​ATG​CAG​GAA​CTG​TAT​AGC​AC CAC​AGC​GTC​TAG​CAC​CTC​TT 
DHX58 GGG​CCT​CCA​AAC​TCG​ATG​G TTC​TGG​GGT​GAC​ATG​ATG​CAC 
MX1 GTT​TCC​GAA​GTG​GAC​ATC​GCA CTG​CAC​AGG​TTG​TTC​TCA​GC 
ISG15 CGC​AGA​TCA​CCC​AGA​AGA​TCG TTC​GTC​GCA​TTT​GTC​CAC​CA 
OAS1 GAA​GGC​AGC​TCA​CGA​AAC​C AGGCCTCAGCCTCTTGTG 
IFIT1 TGT​TGA​AGC​AGA​AGC​ACA​CA TCT​ACG​CGA​TGT​TTC​CTA​CG 
MX2 CAG​AGG​CAG​CGG​AAT​CGT​AA TGA​AGC​TCT​AGC​TCG​GTG​TTC 
IFI44 GGT​GGG​CAC​TAA​TAC​AAC​TGG CAC​ACA​GAA​TAA​ACG​GCA​GGT​A 
OAS1 TGT​CCA​AGG​TGG​TAA​AGG​GTG CCG​GCG​ATT​TAA​CTG​ATC​CTG 
OAS2 ACG​TGA​CAT​CCT​CGA​TAA​AAC​TG GAA​CCC​ATC​AAG​GGA​CTT​CTG 
DDX60 CAG​CTC​CAA​TGA​AAT​GGT​GCC CTC​AGG​GGT​TTA​TGA​GAA​TGC​C 
IDH2 CGC​CAC​TAT​GCC​GAC​AAA​AG ACT​GCC​AGA​TAA​TAC​GGG​TCA 
STAT1 CAA​TGG​AAC​TTG​ATG​GCC​CTA TTC​TAC​AGA​GCC​CAC​TAT​CCG 
IRF1 ATG​CCC​ATC​ACT​CGG​ATG​C CCC​TGC​TTT​GTA​TCG​GCC​TG 
IRF5 GGG​CTT​CAA​TGG​GTC​AAC​G GCC​TTC​GGT​GTA​TTT​CCC​TG 
IRF7 GCT​GGA​CGT​GAC​CAT​CAT​GTA GGG​CCG​TAT​AGG​AAC​GTG​C 
IRF9 GCC​CTA​CAA​GGT​GTA​TCA​GTT​G TGC​TGT​CGC​TTT​GAT​GGT​ACT 
CD274 (PD-L1) TGG​CAT​TTG​CTG​AAC​GCA​TTT TGC​AGC​CAG​GTC​TAA​TTG​TTT​T 
CD274-L2A TAC​AGC​TGA​ATT​GGT​CAT​CCC​A AGG​CAG​ACA​TCA​TGC​TAG​GTG 
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