Table 1.
Primers for qRT-PCR detection of eRNA and mRNA
GeneForward primer, 5′–3′Reverse primer, 5′–3′Genomic coordinates
Gdf15 eRNA −2.6 kb TAG​GAT​CCC​ACT​TCG​CCA​GG TTA​ACC​CCC​AAG​TGA​CAC​CC chr8: 70633546–70633793 
Gdf15 eRNA −3.6 kb GAC​ATC​TCC​CCT​CGG​GTT​CTA CAC​TAC​ACC​ACA​GCA​CCA​GC chr8: 70634857–70634974 
Gdf15 mRNA GCT​GTC​CGG​ATA​CTC​AGT​CC CTT​CAG​GGG​CCT​AGT​GAT​GTC - 
Gdf3 mRNA GGGTGTTCGTGGGAACCT CCA​TCT​TGG​AAA​GGT​TTC​TGT​G - 
Ppia mRNA GCG​TCT​CCT​TCG​AGC​TGT​TT ACC​ACC​CTG​GCA​CAT​GAA​TC - 
Myh2 mRNA TCC​AAG​TTC​CGC​AAG​ATC​CA GCG​CAT​GAC​CAA​AGG​TTT​CA - 
Pax7 mRNA GGC​ACA​GAG​GAC​CAA​GCT​C GCACGCCGGTTACTGAAC - 
Rpl32 mRNA ACA​TTT​GCC​CTG​AAT​GTG​GT ATC​CTC​TTG​CCC​TGA​TCC​TT - 
Igf1 mRNA TGG​ATG​CTC​TTC​AGT​TCG​TG GCA​ACA​CTC​ATC​CAC​AAT​GC - 
Cd36 mRNA TTG​TAC​CTA​TAC​TGT​GGC​TAA​ATG​AGA CTT​GTG​TTT​TGA​ACA​TTT​CTG​CTT - 
Rxrα mRNA ACA​TTT​CCT​GCC​GCT​CGA​CTT TGA​TGA​CAG​AGA​AGG​GCG​GA - 
Gpnmb v1 mRNA ACGGCAGGTGGAAGGACT CGG​TGA​GTC​ACT​GGT​CAG​G - 
Gpnmb v2 mRNA AGC​CAA​TAG​GAA​ACT​GCC​CC AAC​AAC​AGT​TCC​CAG​CCA​CA - 
GeneForward primer, 5′–3′Reverse primer, 5′–3′Genomic coordinates
Gdf15 eRNA −2.6 kb TAG​GAT​CCC​ACT​TCG​CCA​GG TTA​ACC​CCC​AAG​TGA​CAC​CC chr8: 70633546–70633793 
Gdf15 eRNA −3.6 kb GAC​ATC​TCC​CCT​CGG​GTT​CTA CAC​TAC​ACC​ACA​GCA​CCA​GC chr8: 70634857–70634974 
Gdf15 mRNA GCT​GTC​CGG​ATA​CTC​AGT​CC CTT​CAG​GGG​CCT​AGT​GAT​GTC - 
Gdf3 mRNA GGGTGTTCGTGGGAACCT CCA​TCT​TGG​AAA​GGT​TTC​TGT​G - 
Ppia mRNA GCG​TCT​CCT​TCG​AGC​TGT​TT ACC​ACC​CTG​GCA​CAT​GAA​TC - 
Myh2 mRNA TCC​AAG​TTC​CGC​AAG​ATC​CA GCG​CAT​GAC​CAA​AGG​TTT​CA - 
Pax7 mRNA GGC​ACA​GAG​GAC​CAA​GCT​C GCACGCCGGTTACTGAAC - 
Rpl32 mRNA ACA​TTT​GCC​CTG​AAT​GTG​GT ATC​CTC​TTG​CCC​TGA​TCC​TT - 
Igf1 mRNA TGG​ATG​CTC​TTC​AGT​TCG​TG GCA​ACA​CTC​ATC​CAC​AAT​GC - 
Cd36 mRNA TTG​TAC​CTA​TAC​TGT​GGC​TAA​ATG​AGA CTT​GTG​TTT​TGA​ACA​TTT​CTG​CTT - 
Rxrα mRNA ACA​TTT​CCT​GCC​GCT​CGA​CTT TGA​TGA​CAG​AGA​AGG​GCG​GA - 
Gpnmb v1 mRNA ACGGCAGGTGGAAGGACT CGG​TGA​GTC​ACT​GGT​CAG​G - 
Gpnmb v2 mRNA AGC​CAA​TAG​GAA​ACT​GCC​CC AAC​AAC​AGT​TCC​CAG​CCA​CA - 
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