Table 2.
List of DNA oligos synthesized for generating lentiviral miR RNAi expression vectors
NamemiR RNAi oligo sequence (5′–3′)
mRaptor_Sh1_top TGC​TGA​TAA​GAG​GCG​ACT​GCA​GCA​TCG​TTT​TGG​CCA​CTG​ACT​GACGAT​GCT​GCT​CGC​CTC​TTA​T 
mRaptor_Sh1_bottom CCT​GAT​AAG​AGG​CGA​GCA​GCA​TCG​TCA​GTC​AGT​GGC​CAA​AAC​GAT​GCT​GCA​GTC​GCC​TCT​TAT​C 
mRaptor_Sh2_top TGC​TGA​ACA​GTC​GTA​GAC​AAA​GAT​GGG​TTT​TGG​CCA​CTG​ACT​GACCCA​TCT​TTC​TAC​GAC​TGT​T 
mRaptor_Sh2_bottom CCT​GAA​CAG​TCG​TAG​AAA​GAT​GGG​TCA​GTC​AGT​GGC​CAA​AAC​CCA​TCT​TTG​TCT​ACG​ACT​GTT​C 
mRaptor_Sh3_top TGC​TGT​AAA​CAA​ACT​TGC​CAC​GAG​CAG​TTT​TGG​CCA​CTG​ACT​GACTGC​TCG​TGA​AGT​TTG​TTT​A 
mRaptor_Sh3_bottom CCT​GTA​AAC​AAA​CTT​CAC​GAG​CAG​TCA​GTC​AGT​GGC​CAA​AAC​TGC​TCG​TGG​CAA​GTT​TGT​TTA​C 
mRaptor_Sh4_top TGC​TGA​TTA​CAG​CAA​GAA​TGA​AGG​CTG​TTT​TGG​CCA​CTG​ACT​GACAGC​CTT​CAC​TTG​CTG​TAA​T 
mRaptor_Sh4_bottom CCT​GAT​TAC​AGC​AAG​TGA​AGG​CTG​TCA​GTC​AGT​GGC​CAA​AAC​AGC​CTT​CAT​TCT​TGC​TGT​AAT​C 
mRaptor_Sh5_top TGC​TGA​TTA​CAT​TCA​CAG​ACT​CGG​GCG​TTT​TGG​CCA​CTG​ACT​GACGCC​CGA​GTG​TGA​ATG​TAA​T 
mRaptor_Sh5_bottom CCT​GAT​TAC​ATT​CAC​ACT​CGG​GCG​TCA​GTC​AGT​GGC​CAA​AAC​GCC​CGA​GTC​TGT​GAA​TGT​AAT​C 
mRictor_Sh1_top TGC​TGT​CAT​CTT​TCT​GAC​TAA​GCG​AAG​TTT​TGG​CCA​CTG​ACT​GACTTC​GCT​TAC​AGA​AAG​ATG​A 
mRictor_Sh1_bottom CCT​GTC​ATC​TTT​CTG​TAA​GCG​AAG​TCA​GTC​AGT​GGC​CAA​AAC​TTC​GCT​TAG​TCA​GAA​AGA​TGA​C 
mRictor_Sh2_top TGC​TGA​GAA​CTA​GGA​AAC​AAG​GAA​GCG​TTT​TGG​CCA​CTG​ACT​GACGCT​TCC​TTT​TCC​TAG​TTC​T 
mRictor_Sh2_bottom CCT​GAG​AAC​TAG​GAA​AAG​GAA​GCG​TCA​GTC​AGT​GGC​CAA​AAC​GCT​TCC​TTG​TTT​CCT​AGT​TCT​C 
mRictor_Sh3_top TGC​TGT​ACA​TCA​GCT​CGC​ACA​TAC​TGG​TTT​TGG​CCA​CTG​ACT​GACCAG​TAT​GTG​AGC​TGA​TGT​A 
mRictor_Sh3_bottom CCT​GTA​CAT​CAG​CTC​ACA​TAC​TGG​TCA​GTC​AGT​GGC​CAA​AAC​CAG​TAT​GTG​CGA​GCT​GAT​GTA​C 
mRictor_Sh4_top TGC​TGA​TTA​AGG​AGA​CAC​TGA​AAG​ACG​TTT​TGG​CCA​CTG​ACT​GACGTC​TTT​CAG​TCT​CCT​TAA​T 
mRictor_Sh4_bottom CCT​GAT​TAA​GGA​GAC​TGA​AAG​ACG​TCA​GTC​AGT​GGC​CAA​AAC​GTC​TTT​CAG​TGT​CTC​CTT​AAT​C 
mRictor_Sh5_top TGC​TGA​ATG​ATT​CCC​ATC​TTA​CTG​GCG​TTT​TGG​CCA​CTG​ACT​GACGCC​AGT​AAT​GGG​AAT​CAT​T 
mRictor_Sh5_bottom CCT​GAA​TGA​TTC​CCA​TTA​CTG​GCG​TCA​GTC​AGT​GGC​CAA​AAC​GCC​AGT​AAG​ATG​GGA​ATC​ATT​C 
mNeg_Sh_top TGC​TGA​AAT​GTA​CTG​CGC​GTG​GAG​ACG​TTT​TGG​CCA​CTG​ACT​GACGTC​TCC​ACG​CAG​TAC​ATT​T 
mNeg_Sh_bottom CCT​GAA​ATG​TAC​TGC​GTG​GAG​ACG​TCA​GTC​AGT​GGC​CAA​AAC​GTC​TCC​ACG​CGC​AGT​ACA​TTT​C 
LacZ_Sh_top TGC​TGA​AAT​CGC​TGA​TTT​GTG​TAG​TCG​TTT​TGG​CCA​CTG​ACT​GACGAC​TAC​ACA​TCA​GCG​ATT​T 
LacZ_Sh_bottom CCT​GAA​ATC​GCT​GAT​GTG​TAG​TCG​TCA​GTC​AGT​GGC​CAA​AAC​GAC​TAC​ACA​AAT​CAG​CGA​TTT​C 
NamemiR RNAi oligo sequence (5′–3′)
mRaptor_Sh1_top TGC​TGA​TAA​GAG​GCG​ACT​GCA​GCA​TCG​TTT​TGG​CCA​CTG​ACT​GACGAT​GCT​GCT​CGC​CTC​TTA​T 
mRaptor_Sh1_bottom CCT​GAT​AAG​AGG​CGA​GCA​GCA​TCG​TCA​GTC​AGT​GGC​CAA​AAC​GAT​GCT​GCA​GTC​GCC​TCT​TAT​C 
mRaptor_Sh2_top TGC​TGA​ACA​GTC​GTA​GAC​AAA​GAT​GGG​TTT​TGG​CCA​CTG​ACT​GACCCA​TCT​TTC​TAC​GAC​TGT​T 
mRaptor_Sh2_bottom CCT​GAA​CAG​TCG​TAG​AAA​GAT​GGG​TCA​GTC​AGT​GGC​CAA​AAC​CCA​TCT​TTG​TCT​ACG​ACT​GTT​C 
mRaptor_Sh3_top TGC​TGT​AAA​CAA​ACT​TGC​CAC​GAG​CAG​TTT​TGG​CCA​CTG​ACT​GACTGC​TCG​TGA​AGT​TTG​TTT​A 
mRaptor_Sh3_bottom CCT​GTA​AAC​AAA​CTT​CAC​GAG​CAG​TCA​GTC​AGT​GGC​CAA​AAC​TGC​TCG​TGG​CAA​GTT​TGT​TTA​C 
mRaptor_Sh4_top TGC​TGA​TTA​CAG​CAA​GAA​TGA​AGG​CTG​TTT​TGG​CCA​CTG​ACT​GACAGC​CTT​CAC​TTG​CTG​TAA​T 
mRaptor_Sh4_bottom CCT​GAT​TAC​AGC​AAG​TGA​AGG​CTG​TCA​GTC​AGT​GGC​CAA​AAC​AGC​CTT​CAT​TCT​TGC​TGT​AAT​C 
mRaptor_Sh5_top TGC​TGA​TTA​CAT​TCA​CAG​ACT​CGG​GCG​TTT​TGG​CCA​CTG​ACT​GACGCC​CGA​GTG​TGA​ATG​TAA​T 
mRaptor_Sh5_bottom CCT​GAT​TAC​ATT​CAC​ACT​CGG​GCG​TCA​GTC​AGT​GGC​CAA​AAC​GCC​CGA​GTC​TGT​GAA​TGT​AAT​C 
mRictor_Sh1_top TGC​TGT​CAT​CTT​TCT​GAC​TAA​GCG​AAG​TTT​TGG​CCA​CTG​ACT​GACTTC​GCT​TAC​AGA​AAG​ATG​A 
mRictor_Sh1_bottom CCT​GTC​ATC​TTT​CTG​TAA​GCG​AAG​TCA​GTC​AGT​GGC​CAA​AAC​TTC​GCT​TAG​TCA​GAA​AGA​TGA​C 
mRictor_Sh2_top TGC​TGA​GAA​CTA​GGA​AAC​AAG​GAA​GCG​TTT​TGG​CCA​CTG​ACT​GACGCT​TCC​TTT​TCC​TAG​TTC​T 
mRictor_Sh2_bottom CCT​GAG​AAC​TAG​GAA​AAG​GAA​GCG​TCA​GTC​AGT​GGC​CAA​AAC​GCT​TCC​TTG​TTT​CCT​AGT​TCT​C 
mRictor_Sh3_top TGC​TGT​ACA​TCA​GCT​CGC​ACA​TAC​TGG​TTT​TGG​CCA​CTG​ACT​GACCAG​TAT​GTG​AGC​TGA​TGT​A 
mRictor_Sh3_bottom CCT​GTA​CAT​CAG​CTC​ACA​TAC​TGG​TCA​GTC​AGT​GGC​CAA​AAC​CAG​TAT​GTG​CGA​GCT​GAT​GTA​C 
mRictor_Sh4_top TGC​TGA​TTA​AGG​AGA​CAC​TGA​AAG​ACG​TTT​TGG​CCA​CTG​ACT​GACGTC​TTT​CAG​TCT​CCT​TAA​T 
mRictor_Sh4_bottom CCT​GAT​TAA​GGA​GAC​TGA​AAG​ACG​TCA​GTC​AGT​GGC​CAA​AAC​GTC​TTT​CAG​TGT​CTC​CTT​AAT​C 
mRictor_Sh5_top TGC​TGA​ATG​ATT​CCC​ATC​TTA​CTG​GCG​TTT​TGG​CCA​CTG​ACT​GACGCC​AGT​AAT​GGG​AAT​CAT​T 
mRictor_Sh5_bottom CCT​GAA​TGA​TTC​CCA​TTA​CTG​GCG​TCA​GTC​AGT​GGC​CAA​AAC​GCC​AGT​AAG​ATG​GGA​ATC​ATT​C 
mNeg_Sh_top TGC​TGA​AAT​GTA​CTG​CGC​GTG​GAG​ACG​TTT​TGG​CCA​CTG​ACT​GACGTC​TCC​ACG​CAG​TAC​ATT​T 
mNeg_Sh_bottom CCT​GAA​ATG​TAC​TGC​GTG​GAG​ACG​TCA​GTC​AGT​GGC​CAA​AAC​GTC​TCC​ACG​CGC​AGT​ACA​TTT​C 
LacZ_Sh_top TGC​TGA​AAT​CGC​TGA​TTT​GTG​TAG​TCG​TTT​TGG​CCA​CTG​ACT​GACGAC​TAC​ACA​TCA​GCG​ATT​T 
LacZ_Sh_bottom CCT​GAA​ATC​GCT​GAT​GTG​TAG​TCG​TCA​GTC​AGT​GGC​CAA​AAC​GAC​TAC​ACA​AAT​CAG​CGA​TTT​C 

Target sequences are denoted in bold font.

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