Table 1. Parameters of dynamic... | Rockefeller University Press

Table 1.

Parameters of dynamic instability for astral microtubules in HU-arrested cells

	Tub1 only	WT-Tub1	WT-Tub3	Tub3 only
Polymerization rate (μm/min)	2.11 ± 0.11 (52)	2.18 ± 0.08 (77)	2.17 ± 0.13 (55)	2.11 ± 0.08 (66)
Depolymerization rate (μm/min)	5.17 ± 0.21 (53)	4.74 ± 0.18 (72)	4.79 ± 0.02 (61)	3.83 ± 0.13 (57)^a
Catastrophe frequency (min⁻¹)	0.97 (46)	0.96 (65)	1.06 (51)	1.02 (49)
Rescue frequency (min⁻¹)	0.12 (3)	0.39 (15)	0.10 (3)	0.13 (4)
Polymerization duration, s	50.4 ± 4 (52)	49.2 ± 3 (77)	50.3 ± 4 (55)	31.8 ± 2 (66)^b
Depolymerization duration, s	28.3 ± 2 (53)	32.0 ± 1 (72)	30.0 ± 1 (61)	36.3 ± 2 (57)
Dynamicity, tubulin/s	86.28	84.70	88.16	71.96
Time spent polymerizing, %	60.39	59.37	58.64	51.06
Time spent depolymerizing, %	34.63	36.16	38.73	38.63
Time spent attenuated, %	4.98	4.47	2.63	10.31
Total microtubules observed	52	57	58	54
Total time observed, s	4,337	6,375	4,720	4,693

	Tub1 only	WT-Tub1	WT-Tub3	Tub3 only
Polymerization rate (μm/min)	2.11 ± 0.11 (52)	2.18 ± 0.08 (77)	2.17 ± 0.13 (55)	2.11 ± 0.08 (66)
Depolymerization rate (μm/min)	5.17 ± 0.21 (53)	4.74 ± 0.18 (72)	4.79 ± 0.02 (61)	3.83 ± 0.13 (57)^a
Catastrophe frequency (min⁻¹)	0.97 (46)	0.96 (65)	1.06 (51)	1.02 (49)
Rescue frequency (min⁻¹)	0.12 (3)	0.39 (15)	0.10 (3)	0.13 (4)
Polymerization duration, s	50.4 ± 4 (52)	49.2 ± 3 (77)	50.3 ± 4 (55)	31.8 ± 2 (66)^b
Depolymerization duration, s	28.3 ± 2 (53)	32.0 ± 1 (72)	30.0 ± 1 (61)	36.3 ± 2 (57)
Dynamicity, tubulin/s	86.28	84.70	88.16	71.96
Time spent polymerizing, %	60.39	59.37	58.64	51.06
Time spent depolymerizing, %	34.63	36.16	38.73	38.63
Time spent attenuated, %	4.98	4.47	2.63	10.31
Total microtubules observed	52	57	58	54
Total time observed, s	4,337	6,375	4,720	4,693

Results are presented as mean ± SEM, with sample number shown in parentheses.

P ≤ 0.001 versus Tub1 only, WT-Tub 1, and WT-Tub3 by unpaired, two-tailed Student’s t test.

P ≤ 0.01 versus Tub1 only and WT-Tub3; P ≤ 0.001 versus WT-Tub1 by unpaired, two-tailed Student’s t test.