PSF, point spread function. Shown are the parameters of the “point-source detection” particle detection algorithm in u-track that vary between channels and datasets. Two other parameters that change (namely “Max Fit Adjust” and “Fit Window Size”) are not shown because they were given their default values of 2 × PSF sigma and 4 × PSF sigma, respectively. The paxillin channel has N/A parameters because it was not detected using point-source detection due to its non-punctate nature.