Primary and secondary antibodies and their dilutions used for IF imaging
| Antibody . | Host, clonality . | Manufacturer (identifier) . | Dilution used . |
|---|---|---|---|
| Unconjugated primary | |||
| Anti TfR | Rabbit, polyclonal | Abcam, ab84036 | 1:200 |
| Anti TSAd (OTI4F3) | Mouse, monoclonal | Invitrogen, MA5-25894 | 1:100 |
| Anti activated β1-integrin (P4G11) | Mouse, monoclonal | Sigma-Aldrich, MAB1951 | 1:10,000 |
| Anti pFAK (pY397) (EP2160Y) | Rabbit, monoclonal | Abcam, ab81298 | 1:10,000 |
| Anti VEGFR-2 (55B11) | Rabbit, monoclonal | Cell Signaling Technology, #2479 | 1:400 |
| Conjugated primary | |||
| Alexa Fluor 647 Anti-Paxillin (Y113) | Rabbit, monoclonal | Abcam, ab246719 | 1:100 |
| Alexa Fluor 647 Anti-CHC (X22) | Mouse, monoclonal | Invitrogen, MA1-065-A647 | 1:2,000 |
| Secondary | |||
| Alexa Fluor 488 Anti-Mouse IgG secondary | Goat, polyclonal | Invitrogen, A-11029 | 1:1,000 |
| Alexa Fluor 546 Anti-Rabbit IgG secondary | Goat, polyclonal | Invitrogen, A-11010 | 1:1,000 |
| Alexa Fluor 488 Anti-Rabbit IgG secondary | Goat, polyclonal | Invitrogen, A-11008 | 1:10,000 |
| Alexa Fluor 546 Anti-Mouse IgG secondary | Goat, polyclonal | Invitrogen, A-11003 | 1:10,000 |
| Antibody . | Host, clonality . | Manufacturer (identifier) . | Dilution used . |
|---|---|---|---|
| Unconjugated primary | |||
| Anti TfR | Rabbit, polyclonal | Abcam, ab84036 | 1:200 |
| Anti TSAd (OTI4F3) | Mouse, monoclonal | Invitrogen, MA5-25894 | 1:100 |
| Anti activated β1-integrin (P4G11) | Mouse, monoclonal | Sigma-Aldrich, MAB1951 | 1:10,000 |
| Anti pFAK (pY397) (EP2160Y) | Rabbit, monoclonal | Abcam, ab81298 | 1:10,000 |
| Anti VEGFR-2 (55B11) | Rabbit, monoclonal | Cell Signaling Technology, #2479 | 1:400 |
| Conjugated primary | |||
| Alexa Fluor 647 Anti-Paxillin (Y113) | Rabbit, monoclonal | Abcam, ab246719 | 1:100 |
| Alexa Fluor 647 Anti-CHC (X22) | Mouse, monoclonal | Invitrogen, MA1-065-A647 | 1:2,000 |
| Secondary | |||
| Alexa Fluor 488 Anti-Mouse IgG secondary | Goat, polyclonal | Invitrogen, A-11029 | 1:1,000 |
| Alexa Fluor 546 Anti-Rabbit IgG secondary | Goat, polyclonal | Invitrogen, A-11010 | 1:1,000 |
| Alexa Fluor 488 Anti-Rabbit IgG secondary | Goat, polyclonal | Invitrogen, A-11008 | 1:10,000 |
| Alexa Fluor 546 Anti-Mouse IgG secondary | Goat, polyclonal | Invitrogen, A-11003 | 1:10,000 |