Table 1. Primers used for PCR-based cDNA... | Rockefeller University Press

Table 1.

Primers used for PCR-based cDNA amplification with TCR-specific primers

Primer name	Sequence (5′–3′)
Common forward primer	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC
Reverse primers
TCR α outer primer	TGAAGATATCTTGGCAGGTGA
TCR α inner primer with R2 adduct	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTCCTGAGACCGAGGATCT
TCR β outer primer	GCGGAAGTGGTTTCGAGGAT
TCR β inner primer with R2 adduct	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAGACCTTGGGTGGAGTCACA
Common reverse primer with 10× SCS sample ID	CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT

Primer name	Sequence (5′–3′)
Common forward primer	AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC
Reverse primers
TCR α outer primer	TGAAGATATCTTGGCAGGTGA
TCR α inner primer with R2 adduct	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGTCCTGAGACCGAGGATCT
TCR β outer primer	GCGGAAGTGGTTTCGAGGAT
TCR β inner primer with R2 adduct	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAGACCTTGGGTGGAGTCACA
Common reverse primer with 10× SCS sample ID	CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT