Table 1. Summary of results from... | Rockefeller University Press

Table 1.

Summary of results from zebrafish studies evaluating the contribution of primitive myeloid cells to inflammatory cytokines involved in HSC emergence

Intervention	Cells affected	Effect on HSPC	Effect on cytokines	Reference
Irf8^a morpholino	↓ Csf1ra⁺ macrophages	↓ Runx1⁺ in AGM		Li et al., 2014
Irf8^a morpholino	↓ Csf1ra⁺ macrophages	↓ Cd41:Gfp⁺ in CHT		Li et al., 2014
	↓ Mpeg1⁺ macrophages	↑ Runx1⁺ in AGM	↑ TNFα	Espín-Palazón et al., 2014
	↓ Mpeg1⁺ macrophages	↑ Kdrl⁺;cMyb⁺ in AGM	↑ TNFα	Espín-Palazón et al., 2014
	↓ Mfap4⁺ macrophages	No effect	↓ IL-1β; ↓ G-CSF; ↑ TNFα	He et al., 2015
	↑ Lyz⁺ neutrophils	No effect	↓ IL-1β; ↓ G-CSF; ↑ TNFα	He et al., 2015
	↑ Mpx⁺ neutrophils	↑ Runx1⁺ in AGM	↑ IL-6	Tie et al., 2019
Cebp1^b morpholino	↓ Lyz⁺ neutrophils	↓ Runx1⁺ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1b^c morpholino	↓ Mfap4⁺ macrophages	↓ Runx1⁺ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1b^c morpholino	↓ Lyz⁺ neutrophils	↓ Runx1⁺ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1b^d morpholino	↓ l-Plastin⁺ leukocytes	↓ Kdrl⁺;cMyb⁺ in AGM	↓ TNFα	Espín-Palazón et al., 2014
Spi1b^d morpholino	↓ Mpx⁺ neutrophils	↓ Kdrl⁺;cMyb⁺ in AGM	↓ TNFα	Espín-Palazón et al., 2014
	↓ l-Plastin⁺ leukocytes	↓ Runx1⁺ in AGM	↓ IL-6	Tie et al., 2019
Spi1a^e/Spi1b^d morpholino	↓ Irf8⁺ macrophages	↓ Runx1⁺ in AGM	Unknown	Li et al., 2014
Spi1a^e/Spi1b^d morpholino	↓ Mpo⁺ granulocytes	↓ Cd41:Gfp⁺ in CHT	Unknown	Li et al., 2014
	↓↓ Mfap4⁺ macrophages	↓ Runx1⁺ in AGM	Unknown	Frame et al., 2020
	↓ Mpx⁺ neutrophils	↓ Cd41:Gfp⁺ in CHT	Unknown	Frame et al., 2020
Mpeg:NTR^f + MTZ	↓ Mpeg1⁺ macrophages	↓ cMyb⁺ in CHT	Unknown	Frame et al., 2020
Mpeg:Dnikbaa^g	↓ Mpeg1⁺ macrophages	↓ cMyb⁺ in CHT	Unknown	Frame et al., 2020

Intervention	Cells affected	Effect on HSPC	Effect on cytokines	Reference
Irf8^a morpholino	↓ Csf1ra⁺ macrophages	↓ Runx1⁺ in AGM		Li et al., 2014
Irf8^a morpholino	↓ Csf1ra⁺ macrophages	↓ Cd41:Gfp⁺ in CHT		Li et al., 2014
	↓ Mpeg1⁺ macrophages	↑ Runx1⁺ in AGM	↑ TNFα	Espín-Palazón et al., 2014
	↓ Mpeg1⁺ macrophages	↑ Kdrl⁺;cMyb⁺ in AGM	↑ TNFα	Espín-Palazón et al., 2014
	↓ Mfap4⁺ macrophages	No effect	↓ IL-1β; ↓ G-CSF; ↑ TNFα	He et al., 2015
	↑ Lyz⁺ neutrophils	No effect	↓ IL-1β; ↓ G-CSF; ↑ TNFα	He et al., 2015
	↑ Mpx⁺ neutrophils	↑ Runx1⁺ in AGM	↑ IL-6	Tie et al., 2019
Cebp1^b morpholino	↓ Lyz⁺ neutrophils	↓ Runx1⁺ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1b^c morpholino	↓ Mfap4⁺ macrophages	↓ Runx1⁺ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1b^c morpholino	↓ Lyz⁺ neutrophils	↓ Runx1⁺ in AGM	↓ IL-1β; ↓ G-CSF; ↓ TNFα	He et al., 2015
Spi1b^d morpholino	↓ l-Plastin⁺ leukocytes	↓ Kdrl⁺;cMyb⁺ in AGM	↓ TNFα	Espín-Palazón et al., 2014
Spi1b^d morpholino	↓ Mpx⁺ neutrophils	↓ Kdrl⁺;cMyb⁺ in AGM	↓ TNFα	Espín-Palazón et al., 2014
	↓ l-Plastin⁺ leukocytes	↓ Runx1⁺ in AGM	↓ IL-6	Tie et al., 2019
Spi1a^e/Spi1b^d morpholino	↓ Irf8⁺ macrophages	↓ Runx1⁺ in AGM	Unknown	Li et al., 2014
Spi1a^e/Spi1b^d morpholino	↓ Mpo⁺ granulocytes	↓ Cd41:Gfp⁺ in CHT	Unknown	Li et al., 2014
	↓↓ Mfap4⁺ macrophages	↓ Runx1⁺ in AGM	Unknown	Frame et al., 2020
	↓ Mpx⁺ neutrophils	↓ Cd41:Gfp⁺ in CHT	Unknown	Frame et al., 2020
Mpeg:NTR^f + MTZ	↓ Mpeg1⁺ macrophages	↓ cMyb⁺ in CHT	Unknown	Frame et al., 2020
Mpeg:Dnikbaa^g	↓ Mpeg1⁺ macrophages	↓ cMyb⁺ in CHT	Unknown	Frame et al., 2020

Irf8: 5′-AATGTTTCGCTTACTTTGAAAATGG-3′ and 5′-TCAGTCTGCGACCGCCCGAGTTCAT-3′.

Cebp1: 5′-GTCAGACACCGACATGGCTGTGTGT-3′, 5′-GGAGCTGCTGAACTCTACTCGATCT-3′, and 5′-GTCTGACTCCGTCATCGCTGAGTGT-3′.

Pu.1: 5′-CCTCCATTCTGTACGGATGCAGCAT-3′ and 5′-GGTCTTTCTCCTTACCATGCTCTCC-3′.

Pu.1/Spi1b: 5′-GATATACTGATACTCCATTGGTGGT-3′.

Spi1a: 5′-AGCGACTCACGCTGTGGAGGAACT-3′.

Mpeg:NTR: Tg(mpeg1:GAL4;UAS:NTRmCherry) + metronidazole.

Mpeg:Dnikbaa: Tg(mpeg1:GAL4;UAS:nfsB-mCherry;UAS:dnikbaa).

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