Table 1.
Primers used for qRT-PCR
GeneForward primerReverse primer
Becn1 5′-CCC​CAG​GCA​GCA​TTG​ATT​TC-3′ 5′-AGC​AGC​TGG​AGT​TGG​ATG​AC-3′ 
Bnip3 5′-TCG​ACT​TGA​CCA​ATC​CCA​TAT​CC-3′ 5′-TCC​CAG​ACA​CCA​CAA​GAT​ACC-3′ 
Cat 5′-TTC​AGT​GAC​CGA​GGG​ATT​CC-3′ 5′-TTC​CTG​AGC​AAG​CCT​TCC​TG-3′ 
Ccl2 5′-AGG​TGT​CCC​AAA​GAA​GCT​GT-3′ 5′-ACA​GAA​GTG​CTT​GAG​GTG​GT-3′ 
Cd36 5′-ACA​CAC​CAC​CAT​TTC​TTC​TCC-3′ 5′-GCT​CCT​TGG​CAT​GGT​AGA​GA-3′ 
Cpt1a 5′-TTA​TGT​GAG​TGA​CTG​GTG​GGA​GG-3′ 5′-ATG​GGT​TGG​GGT​GAT​GTA​GAG​C-3′ 
Cpt1b 5′-AGG​CTC​CAG​GGT​TCA​GAA​AG-3′ 5′-AAC​ATG​TAC​CGC​CTA​GCC​AT-3′ 
Crp 5′-AGC​TTC​TCT​CGG​ACT​TTT​GGT-3′ 5′-AAT​CGT​ACT​TCA​GCA​CCA​CCC-3′ 
Ddit3 5′-TCT​CAT​CCC​CAG​GAA​ACG​AAG-3′ 5′-TGT​GCG​TGT​GAC​CTC​TGT​TGG-3′ 
Edn 5′-CCA​AGG​AGC​TCC​AGA​AAC​AG-3′ 5′-GGT​GAG​CGC​ACT​GAC​ATC​TA-3′ 
Fgf21 5′-CCT​CTA​GGT​TTC​TTT​GCC​AAC​AG-3′ 5′-AAG​CTG​CAG​GCC​TCA​GGA​T-3′ 
Hmox1 5′-CAT​CCA​AGC​CGA​GAA​TGC​TG-3′ 5′-CCT​CAG​GGA​AGT​AGA​GTG​GGG-3′ 
Il1a 5′-ACC​CGA​CTT​TGT​TCT​TTG​GTG-3′ 5′-GAG​AAG​ACC​AGC​CCG​TGT​TG-3′ 
Il1b 5′-CAG​TTG​TCT​AAT​GGG​AAC​GTC​A-3′ 5′-GCA​CCT​TCT​TTT​CCT​TCA​TCT​TT-3′ 
Il6 5′-GAC​TTC​CAT​CCA​GTT​GCC​TTC​TTG​G-3′ 5′-CCA​GTT​TGG​TAG​CAT​CCA​TCA​TTT​CT-3′ 
Il17a 5′-ACT​ACC​TCA​ACC​GTT​CCA​CG-3′ 5′-TTC​CCT​CCG​CAT​TGA​CAC​AG-3′ 
Il17f 5′-ACG​TGA​ATT​CCA​GAA​CCG​CT-3′ 5′-TGA​TGC​AGC​CTG​AGT​GTC​TG-3′ 
Il18 5′-GAC​AAA​GAA​AGC​CGC​CTC​AA-3′ 5′-TCA​TTT​CCT​TGA​AGT​TGA​CGC​A-3′ 
Klb 5′-GAT​GAA​GAA​TTT​CCT​AAA​CCA​GGT​T-3′ 5′-AAC​CAA​ACA​CGC​GGA​TTT​C-3′ 
Nos2 5′-GAA​TCT​TGG​AGC​GAG​TTG​TGG-3′ 5′-CAG​GAA​GTA​GGT​GAG​GGC​TTG-3′ 
Pdk4 5′-TGC​CTG​ACC​GCT​TAG​TGA​ACA​C-3′ 5′-CTT​GAG​CCA​TTG​TAG​GGA​CCA​C-3′ 
Ppargc1a 5′-GTA​AAT​CTG​CGG​GAT​GAT​GG-3′ 5′-GGG​TCA​AAA​TCG​TCT​GAG​TTG-3′ 
Rpl13a 5′-GAG​GTC​GGG​TGG​AAG​TAC​CA-3′ 5′-TGC​ATC​TTG​GCC​TTT​TCC​TT-3′ 
Saa1 5′-CTG​ACA​TGA​AGG​AAG​CTA​ACT​GGA-3′ 5′-GCC​GAA​GAA​TTC​CTG​AAA​GGC-3′ 
Saa2 5′-AGG​AAG​CTG​GCT​GGA​AAG​AT-3′ 5′-CAG​TAT​TTG​GCA​GGC​AGT​CC-3′ 
Sod2 5′-CAG​CCT​GAA​CCT​TGG​ACT​CC-3′ 5′-ACA​ACT​CAG​GTC​GCT​CTT​CAG-3′ 
Tgfb1 5′-TGA​GTG​GCT​GTC​TTT​TGA​CG-3′ 5′-GGT​TCA​TGT​CAT​GGA​TGG​TG-3′ 
Tnfa 5′-TCT​GTC​TAC​TGA​ACT​TCG​GGG​TG-3′ 5′-ACT​TGG​TGG​TTT​GCT​ACG​ACG-3′ 
Ucp2 5′-TGC​ACT​CCT​GTG​TTC​TCC​TG-3′ 5′-GGG​ACC​TTC​AAT​CGG​CAA​GA-3′ 
Ulk1 5′-TTG​ACT​CGG​ATG​TTG​CTG​GG-3′ 5′-GTG​GAG​ACC​TGG​CTG​ACT​AC-3′ 
GeneForward primerReverse primer
Becn1 5′-CCC​CAG​GCA​GCA​TTG​ATT​TC-3′ 5′-AGC​AGC​TGG​AGT​TGG​ATG​AC-3′ 
Bnip3 5′-TCG​ACT​TGA​CCA​ATC​CCA​TAT​CC-3′ 5′-TCC​CAG​ACA​CCA​CAA​GAT​ACC-3′ 
Cat 5′-TTC​AGT​GAC​CGA​GGG​ATT​CC-3′ 5′-TTC​CTG​AGC​AAG​CCT​TCC​TG-3′ 
Ccl2 5′-AGG​TGT​CCC​AAA​GAA​GCT​GT-3′ 5′-ACA​GAA​GTG​CTT​GAG​GTG​GT-3′ 
Cd36 5′-ACA​CAC​CAC​CAT​TTC​TTC​TCC-3′ 5′-GCT​CCT​TGG​CAT​GGT​AGA​GA-3′ 
Cpt1a 5′-TTA​TGT​GAG​TGA​CTG​GTG​GGA​GG-3′ 5′-ATG​GGT​TGG​GGT​GAT​GTA​GAG​C-3′ 
Cpt1b 5′-AGG​CTC​CAG​GGT​TCA​GAA​AG-3′ 5′-AAC​ATG​TAC​CGC​CTA​GCC​AT-3′ 
Crp 5′-AGC​TTC​TCT​CGG​ACT​TTT​GGT-3′ 5′-AAT​CGT​ACT​TCA​GCA​CCA​CCC-3′ 
Ddit3 5′-TCT​CAT​CCC​CAG​GAA​ACG​AAG-3′ 5′-TGT​GCG​TGT​GAC​CTC​TGT​TGG-3′ 
Edn 5′-CCA​AGG​AGC​TCC​AGA​AAC​AG-3′ 5′-GGT​GAG​CGC​ACT​GAC​ATC​TA-3′ 
Fgf21 5′-CCT​CTA​GGT​TTC​TTT​GCC​AAC​AG-3′ 5′-AAG​CTG​CAG​GCC​TCA​GGA​T-3′ 
Hmox1 5′-CAT​CCA​AGC​CGA​GAA​TGC​TG-3′ 5′-CCT​CAG​GGA​AGT​AGA​GTG​GGG-3′ 
Il1a 5′-ACC​CGA​CTT​TGT​TCT​TTG​GTG-3′ 5′-GAG​AAG​ACC​AGC​CCG​TGT​TG-3′ 
Il1b 5′-CAG​TTG​TCT​AAT​GGG​AAC​GTC​A-3′ 5′-GCA​CCT​TCT​TTT​CCT​TCA​TCT​TT-3′ 
Il6 5′-GAC​TTC​CAT​CCA​GTT​GCC​TTC​TTG​G-3′ 5′-CCA​GTT​TGG​TAG​CAT​CCA​TCA​TTT​CT-3′ 
Il17a 5′-ACT​ACC​TCA​ACC​GTT​CCA​CG-3′ 5′-TTC​CCT​CCG​CAT​TGA​CAC​AG-3′ 
Il17f 5′-ACG​TGA​ATT​CCA​GAA​CCG​CT-3′ 5′-TGA​TGC​AGC​CTG​AGT​GTC​TG-3′ 
Il18 5′-GAC​AAA​GAA​AGC​CGC​CTC​AA-3′ 5′-TCA​TTT​CCT​TGA​AGT​TGA​CGC​A-3′ 
Klb 5′-GAT​GAA​GAA​TTT​CCT​AAA​CCA​GGT​T-3′ 5′-AAC​CAA​ACA​CGC​GGA​TTT​C-3′ 
Nos2 5′-GAA​TCT​TGG​AGC​GAG​TTG​TGG-3′ 5′-CAG​GAA​GTA​GGT​GAG​GGC​TTG-3′ 
Pdk4 5′-TGC​CTG​ACC​GCT​TAG​TGA​ACA​C-3′ 5′-CTT​GAG​CCA​TTG​TAG​GGA​CCA​C-3′ 
Ppargc1a 5′-GTA​AAT​CTG​CGG​GAT​GAT​GG-3′ 5′-GGG​TCA​AAA​TCG​TCT​GAG​TTG-3′ 
Rpl13a 5′-GAG​GTC​GGG​TGG​AAG​TAC​CA-3′ 5′-TGC​ATC​TTG​GCC​TTT​TCC​TT-3′ 
Saa1 5′-CTG​ACA​TGA​AGG​AAG​CTA​ACT​GGA-3′ 5′-GCC​GAA​GAA​TTC​CTG​AAA​GGC-3′ 
Saa2 5′-AGG​AAG​CTG​GCT​GGA​AAG​AT-3′ 5′-CAG​TAT​TTG​GCA​GGC​AGT​CC-3′ 
Sod2 5′-CAG​CCT​GAA​CCT​TGG​ACT​CC-3′ 5′-ACA​ACT​CAG​GTC​GCT​CTT​CAG-3′ 
Tgfb1 5′-TGA​GTG​GCT​GTC​TTT​TGA​CG-3′ 5′-GGT​TCA​TGT​CAT​GGA​TGG​TG-3′ 
Tnfa 5′-TCT​GTC​TAC​TGA​ACT​TCG​GGG​TG-3′ 5′-ACT​TGG​TGG​TTT​GCT​ACG​ACG-3′ 
Ucp2 5′-TGC​ACT​CCT​GTG​TTC​TCC​TG-3′ 5′-GGG​ACC​TTC​AAT​CGG​CAA​GA-3′ 
Ulk1 5′-TTG​ACT​CGG​ATG​TTG​CTG​GG-3′ 5′-GTG​GAG​ACC​TGG​CTG​ACT​AC-3′ 
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