Table 1.
Summary of mouse studies describing the contribution of different infection-derived inflammatory sources to HSC biology
SourceInflammatory pathwayMolecular mechanismEffect on HSCsReference
Viral     
Lymphocytic choriomeningitis virus IFN-α/β–IFNAR ND 30-fold reduction on CFU (at infection day 3) Binder et al. (1997)  
 IFN-γ–IFNGR IFN-γ–induced SOCS1 inhibits TPO-induced STAT5 phosphorylation and leads to decreased/increased cyclin D1/Cdkn1C Decreased pool recovery and self-renewal capacity (at infection days 4–12) de Bruin et al. (2013)  
 IFN-γ–IFNGR IFN-γ–induced Cebpb in high IFNGR–expressing myeloid-biased HSCs Increased proliferation and myeloid bias associated to decreased self-renewal (at infection day 6) Matatall et al. (2014)  
 IFN-γ–IFNGR–IL-6 Cytotoxic CD8+ T cell–produced IFN-γ activates IL-6 production by BM MSCs, leading to reduced Runx-1 and Cebpa  expression Increased myeloid-differentiation bias on HSPCs Schürch et al. (2014)  
 IFN-α/β–IFNAR and IFN-γ–IFNGR Persistent destruction of CARc networks by virus-specific IFN-producing CD8+ T cells—loss of HSC quiescence–enforcing niche Increased proliferation, decreased pool size, and decreased repopulating capacity Isringhausen et al., 2020 Preprint 
Murine cytomegalovirus Increased IFN-γ, IL-17, and CCL12 levels Inflammatory milieu associates in an IFNAR-independent manner with increased Sca-1 and Evi1 gene expression in HSCs Decreased repopulating capacity and increased myeloid differentiation, after BM viral clearance (at infection day 21) Hirche et al. (2017)  
Bacterial     
Polymicrobial sepsis using cecal ligation and puncture model ND HSC expansion associates with reduced BM cellularity but is independent from MyD88, TRIFF, IFNAR, TNF-α, IL-1, IL-6, prostaglandins, oxidative stress, and super antigen signaling Increased pool size Scumpia et al. (2010)  
Escherichia coli G-CSF/CXCL12 Dual stimulation of NOD1 and TLR4 leads to increased G-CSF and decreased CXCL12 production in radioresistant endothelial cells Increased mobilization of BM-expanded HSCs, reduced repopulating capacity, and increased myeloid-differentiation Burberry et al. (2014)  
 SHH-GLI ERK1/2–SP1–mediated increased SHH expression in Lin+ BM cells leads to higher GLI levels in HSCs Proliferation and myeloid differentiation Shi et al. (2018)  
 IFN-γ–IFNGR ND Increased proliferation and decreased repopulating capacity (4 wk after single bacterial inoculation) Baldridge et al. (2010)  
M. avium IFN-γ–IFNGR IFN-γ–induced Batf2 leading to HSPC terminal differentiation Decreased pool size, repopulating capacity, and increased myeloid differentiation (after six monthly inoculations) Matatall et al. (2016)  
 IFN-γ–IFNGR IFN-γ–induced expression of BST2 (noncanonical E-selectin ligand) displaces HSCs from quiescence-enforcing CARc niche to an activating E-selectin–positive vascular niche Increased proliferation, terminal differentiation, and decreased pool size (after four monthly inoculations) Florez et al. (2020)  
Mycobacterium tuberculosis TNF-α/IL-6 TLR2 and MyD88 bacterial sensing dependent Increased HSPC pool and myeloid differentiation Choi et al. (2011)  
 IFN-α/β–IFNAR IFNAR-dependent reprogramming of HSCs leading to dysregulated iron metabolism, depolarized mitochondrial membrane, and necrosis in myeloid progenitors Decreased pool and reconstitution capacity (up to 1 yr after infection) Khan et al. (2020)  
Ehrlichia muris IFN-γ–IFNGR ND Decreased pool and reconstitution capacity, and increased myeloid differentiation bias (at infection day 8) MacNamara et al. (2011)  
 IFN-α/β–IFNAR Direct sensitization of HSPCs to RIPK1-dependent death and increased HSC proliferative arrest Decreased pool and reconstitution capacity (at infection day 7) Smith et al. (2018)  
Protozoan     
Plasmodium berghei ND ND Increased proliferation and pool size (at infection days 7–10) Vainieri et al. (2016)  
 IFN-γ–IFNGR Loss of BM niche osteoblasts and endothelial cell properties Increased turnover leading to decreased functionality and transcriptional identity Haltalli et al. (2020)  
Fungal     
Candida albicans ND (TNF-α?) TLR2/MyD88 and dectin-1 fungal sensing dependent Increased proliferation and pool size Martínez et al. (2018)  
 IL-1β and GM-CSF/CD131 β-glucan–mediated IL-1β and GM-CSF production by BM cells leads to increased glycolytic pathways and proliferation in HSCs Increased proliferation, pool size, and myeloid differentiation bias (at 7 d after exposure) Mitroulis et al. (2018)  
SourceInflammatory pathwayMolecular mechanismEffect on HSCsReference
Viral     
Lymphocytic choriomeningitis virus IFN-α/β–IFNAR ND 30-fold reduction on CFU (at infection day 3) Binder et al. (1997)  
 IFN-γ–IFNGR IFN-γ–induced SOCS1 inhibits TPO-induced STAT5 phosphorylation and leads to decreased/increased cyclin D1/Cdkn1C Decreased pool recovery and self-renewal capacity (at infection days 4–12) de Bruin et al. (2013)  
 IFN-γ–IFNGR IFN-γ–induced Cebpb in high IFNGR–expressing myeloid-biased HSCs Increased proliferation and myeloid bias associated to decreased self-renewal (at infection day 6) Matatall et al. (2014)  
 IFN-γ–IFNGR–IL-6 Cytotoxic CD8+ T cell–produced IFN-γ activates IL-6 production by BM MSCs, leading to reduced Runx-1 and Cebpa  expression Increased myeloid-differentiation bias on HSPCs Schürch et al. (2014)  
 IFN-α/β–IFNAR and IFN-γ–IFNGR Persistent destruction of CARc networks by virus-specific IFN-producing CD8+ T cells—loss of HSC quiescence–enforcing niche Increased proliferation, decreased pool size, and decreased repopulating capacity Isringhausen et al., 2020 Preprint 
Murine cytomegalovirus Increased IFN-γ, IL-17, and CCL12 levels Inflammatory milieu associates in an IFNAR-independent manner with increased Sca-1 and Evi1 gene expression in HSCs Decreased repopulating capacity and increased myeloid differentiation, after BM viral clearance (at infection day 21) Hirche et al. (2017)  
Bacterial     
Polymicrobial sepsis using cecal ligation and puncture model ND HSC expansion associates with reduced BM cellularity but is independent from MyD88, TRIFF, IFNAR, TNF-α, IL-1, IL-6, prostaglandins, oxidative stress, and super antigen signaling Increased pool size Scumpia et al. (2010)  
Escherichia coli G-CSF/CXCL12 Dual stimulation of NOD1 and TLR4 leads to increased G-CSF and decreased CXCL12 production in radioresistant endothelial cells Increased mobilization of BM-expanded HSCs, reduced repopulating capacity, and increased myeloid-differentiation Burberry et al. (2014)  
 SHH-GLI ERK1/2–SP1–mediated increased SHH expression in Lin+ BM cells leads to higher GLI levels in HSCs Proliferation and myeloid differentiation Shi et al. (2018)  
 IFN-γ–IFNGR ND Increased proliferation and decreased repopulating capacity (4 wk after single bacterial inoculation) Baldridge et al. (2010)  
M. avium IFN-γ–IFNGR IFN-γ–induced Batf2 leading to HSPC terminal differentiation Decreased pool size, repopulating capacity, and increased myeloid differentiation (after six monthly inoculations) Matatall et al. (2016)  
 IFN-γ–IFNGR IFN-γ–induced expression of BST2 (noncanonical E-selectin ligand) displaces HSCs from quiescence-enforcing CARc niche to an activating E-selectin–positive vascular niche Increased proliferation, terminal differentiation, and decreased pool size (after four monthly inoculations) Florez et al. (2020)  
Mycobacterium tuberculosis TNF-α/IL-6 TLR2 and MyD88 bacterial sensing dependent Increased HSPC pool and myeloid differentiation Choi et al. (2011)  
 IFN-α/β–IFNAR IFNAR-dependent reprogramming of HSCs leading to dysregulated iron metabolism, depolarized mitochondrial membrane, and necrosis in myeloid progenitors Decreased pool and reconstitution capacity (up to 1 yr after infection) Khan et al. (2020)  
Ehrlichia muris IFN-γ–IFNGR ND Decreased pool and reconstitution capacity, and increased myeloid differentiation bias (at infection day 8) MacNamara et al. (2011)  
 IFN-α/β–IFNAR Direct sensitization of HSPCs to RIPK1-dependent death and increased HSC proliferative arrest Decreased pool and reconstitution capacity (at infection day 7) Smith et al. (2018)  
Protozoan     
Plasmodium berghei ND ND Increased proliferation and pool size (at infection days 7–10) Vainieri et al. (2016)  
 IFN-γ–IFNGR Loss of BM niche osteoblasts and endothelial cell properties Increased turnover leading to decreased functionality and transcriptional identity Haltalli et al. (2020)  
Fungal     
Candida albicans ND (TNF-α?) TLR2/MyD88 and dectin-1 fungal sensing dependent Increased proliferation and pool size Martínez et al. (2018)  
 IL-1β and GM-CSF/CD131 β-glucan–mediated IL-1β and GM-CSF production by BM cells leads to increased glycolytic pathways and proliferation in HSCs Increased proliferation, pool size, and myeloid differentiation bias (at 7 d after exposure) Mitroulis et al. (2018)  

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