Table 4.
Secondary antibodies and reagents for detection of biotinylated proteins used in this study
ConjugationTarget speciesHost speciesSource and catalogue numberDilutions used
IRDye 800CW Rabbit Goat Licor, 926-32211 WB: 1/10,000 
IRDye 680D Mouse Goat Licor, 926-68070 WB: 1/10,000 
Alexa Fluor 680 Rat Goat Thermo Fisher Scientific, A-21096 WB: 1/10,000 
Alexa Fluor 488 Rabbit Donkey Thermo Fisher Scientific, A21206 IF: 1/500 
Alexa Fluor 488 Mouse Donkey Thermo Fisher Scientific, A21202 IF: 1/500 
Alexa Fluor 647 Rabbit Donkey Thermo Fisher Scientific, A31573 IF: 1/500 
Alexa Fluor 647 Mouse Donkey Thermo Fisher Scientific, A31571 IF: 1/500 
Alexa Fluor 568 Rabbit Donkey Thermo Fisher Scientific, A10042 IF: 1/500 
Alexa Fluor 568 Mouse Donkey Thermo Fisher Scientific, A10037 IF: 1/500 
STAR 635P Rabbit Goat Abberior, ST635P-1002 IF (STED): 1/200 
ATTO 594 Mouse Goat Sigma-Aldrich, 76085 IF (STED): 1/200 
Alexa Fluor 647–streptavidin NA NA Thermo Fisher Scientific, S21374 IF: 1/2,000 
DyLight800-streptavidin NA NA Thermo Fisher Scientific, 21851 WB: 1/15,000 
ConjugationTarget speciesHost speciesSource and catalogue numberDilutions used
IRDye 800CW Rabbit Goat Licor, 926-32211 WB: 1/10,000 
IRDye 680D Mouse Goat Licor, 926-68070 WB: 1/10,000 
Alexa Fluor 680 Rat Goat Thermo Fisher Scientific, A-21096 WB: 1/10,000 
Alexa Fluor 488 Rabbit Donkey Thermo Fisher Scientific, A21206 IF: 1/500 
Alexa Fluor 488 Mouse Donkey Thermo Fisher Scientific, A21202 IF: 1/500 
Alexa Fluor 647 Rabbit Donkey Thermo Fisher Scientific, A31573 IF: 1/500 
Alexa Fluor 647 Mouse Donkey Thermo Fisher Scientific, A31571 IF: 1/500 
Alexa Fluor 568 Rabbit Donkey Thermo Fisher Scientific, A10042 IF: 1/500 
Alexa Fluor 568 Mouse Donkey Thermo Fisher Scientific, A10037 IF: 1/500 
STAR 635P Rabbit Goat Abberior, ST635P-1002 IF (STED): 1/200 
ATTO 594 Mouse Goat Sigma-Aldrich, 76085 IF (STED): 1/200 
Alexa Fluor 647–streptavidin NA NA Thermo Fisher Scientific, S21374 IF: 1/2,000 
DyLight800-streptavidin NA NA Thermo Fisher Scientific, 21851 WB: 1/15,000 

IF, immunofluorescence; NA, not applicable; WB, Western blotting.

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