Table 2.
Primers and oligonucleotides used in this study
NameSequence (5′ to 3′)Purpose
M1-REEP4 CTC​ATG​CTG​TGC​CTC​CCC​TTT​CCC​CAG​GGC​ACC​TCG​CGC​TCC​CTG​AAG​GTT​CGG​ACG​AGG​AAA​AAG​ACT​GTG​CCC​TCA​GAC​GTG​GAC​AGC​TCA​GGT​GGA​GGA​GGT​AGT​G Generation of amplicon for HA-tagging of REEP4 at the endogenous locus with CRISPR/Cas12 (Fueller et al., 2020) 
M2-REEP4 ATA​GCC​CTG​GAG​CCC​TGC​AGG​GCA​CGA​GGT​AAG​AAG​GGG​GCA​GAT​GCA​GCA​GAC​CAA​AAA​ATG​GAC​AGC​TAG​GGT​CTG​CTG​ATC​TAC​AAG​AGT​AGA​AAT​TAG​CTA​GCT​GCA​TCG​GTA​CC Generation of amplicon for HA-tagging of REEP4 at the endogenous locus with CRISPR/Cas12 (Fueller et al., 2020) 
RGP09 AGA​TAC​TGA​GGC​AGT​CCC​CC Forward primer for sequencing of gene-edited REEP4 locus 
RGP10 GAC​TTT​CCA​CAC​CGT​CGA​CAT Reverse primer for sequencing of gene-edited REEP4 locus 
oAS363 GCC​CTG​AAA​ATA​AAG​ATT​CTC​TGT​TGT​GGG​TCC​ACT​ACC​AGA​TCC​GGA​GCT​GTC​CAC​GTC​TGA​GGG​CAC Generation of REEP4-V5-tev-HA (see text) 
oAS365 TCT​TTA​TTT​TCA​GGG​CAG​TGG​TAG​TGG​CAG​CGG​ATA​CCC​ATA​CGA​TGT​TCC​AGA​TTA​C Generation of REEP4-V5-tev-HA (see text) 
oAS416 TCC​GAG​CAG​GGG​ATT​GGG​GAT​AGG​CTT​GCC​TCC​ACT​ACC​AGA​TCC​GGA​GC Generation of REEP4-V5-tev-HA (see text) 
oAS417 AAT​CCC​CTG​CTC​GGA​CTG​GAT​AGC​ACC​CCC​ACA​ACA​GAG​AAT​CTT​TAT​TTT​C Generation of REEP4-V5-tev-HA (see text) 
oAS421 TCC​GGA​TCT​GGT​AGT​GGA​GGC​AAG​CCT​ATC​CCC​AAT​CCC​CTG​CTC​GGA​CTG​GAT​AGC​ACC​CCC​ACA​ACA​GAG​AAT​CTT​TAT​TTT​CAG​GGC Generation of REEP3-V5-tev-HA (see text) 
oAS422 GCC​CTG​AAA​ATA​AAG​ATT​CTC​TGT​TGT​GGG​GGT​GCT​ATC​CAG​TCC​GAG​CAG​GGG​ATT​GGG​GAT​AGG​CTT​GCC​TCC​ACT​ACC​AGA​TCC​GGA Generation of REEP3-V5-tev-HA (see text) 
oAS423 CAC​TAC​CAG​ATC​CGG​AAA​AAT​ACA​CTT​GTG​GTC​GTT​TCT​TC Generation of REEP3-V5-tev-HA (see text) 
oAS424 AAT​CTT​TAT​TTT​CAG​GGC​AGT​GGT​AGT​GGC​AGC​GGA​TAC​CCA​TAC​GAT​GTT​CCA​GAT​TAC Generation of REEP3-V5-tev-HA (see text) 
oAS492 AGT​GGT​AGT​GGC​AGC​GGA​CCC​ACA​ACA​GAG​AAT​CTT​TAT​TTT​CAG​GGC​GGC​AAG​CCT​ATC​CCC​AAT​CCC​CTG​CTC​GGA​CTG​GAT​AGC​ACC​TCC​GGA​TCT​GGT​AGT​GGA Generation of HA-tev-V5-Lap2β (see text) 
oAS493 TCC​ACT​ACC​AGA​TCC​GGA​GGT​GCT​ATC​CAG​TCC​GAG​CAG​GGG​ATT​GGG​GAT​AGG​CTT​GCC​GCC​CTG​AAA​ATA​AAG​ATT​CTC​TGT​TGT​GGG​TCC​GCT​GCC​ACT​ACC​ACT Generation of HA-tev-V5-Lap2β (see text) 
oAS494 GCT​GCC​ACT​ACC​ACT​AGC​GTA​ATC​TGG​AAC​ATC​GTA​TGG​GTA​CAT​GGT​GGC​GAC​CGG​TAG​C Generation of HA-tev-V5-Lap2β (see text) 
oAS495 CGG​ATC​TGG​TAG​TGG​AAT​GCC​GGA​GTT​CCT​AGA​GGA​C Generation of HA-tev-V5-Lap2β (see text) 
AB20 GGC​AGC​GGA​TCC​GGG​TCT​GGC​TCC​GGA​TCA​AAA​GAC​AAT​ACT​GTG​CCT​CTG​AAG​C Amplification of TurboID sequence 
AB21 CTA​TGC​GTA​ATC​CGG​TAC​ATC​GTA​AGG​GTA​GCT​AGC​CTT​TTC​GGC​AGA​C Amplification of TurboID sequence 
AB03 ATG​TAC​CGG​ATT​ACG​CAT​AGT​GTA​CAA​GTA​AAG​CGG​CCG​C Amplification/linearization of REEP4-HA–containing vector 
AB15 CCA​GAC​CCG​GAT​CCG​CTG​CCG​CTG​TCC​ACG​TCT​GAG​GG Amplification/linearization of REEP4-HA–containing vector 
AB22 CGG​ACT​CAG​ATC​TCG​AGC​TCA​TGT​CTG​CGG​CCA​TGA​G Amplification of REEP5 sequence 
AB23 CCA​GAC​CCG​GAT​CCG​CTG​CCG​GTG​CTC​TTC​TTT​TCT​TCA​CCC Amplification of REEP5 sequence 
AB24 ATG​TAC​CGG​ATT​ACG​CAT​AGT​GTA​CAA​GTA​AAG​CGG​CCG​C Amplification of REEP4-HA vector backbone (excluding REEP4-HA) 
AB25 GAG​CTC​GAG​ATC​TGA​GTC​CG Amplification of REEP4-HA vector backbone (excluding REEP4-HA) 
AB26 AGA​AAT​CAG​TCT​GCG​GTC​TGC Amplification of vector backbone (from 3xHA-miniturbo-NLS; Addgene) 
AB27 TGCGTAGTCTGGGACGTC Amplification of vector backbone (from 3xHA-miniturbo-NLS; Addgene) 
AB28 ATG​ACG​TCC​CAG​ACT​ACG​CAA​AAG​ACA​ATA​CTG​TGC​CTC​TGA​AGC Amplification of TurboID-NLS 
AB29 CTTTTCGGCAGACCGCAG Amplification of TurboID-NLS 
AB08 CTA​GAG​GGC​CCG​TTT​AAA​CC Amplification/linearization of pcDNA5/FRT/TO vector 
AB09 CAA​GCT​TAA​GTT​TAA​ACG​CTA​GAG​TCC Amplification/linearization of pcDNA5/FRT/TO vector 
AB10 AGC​GTT​TAA​ACT​TAA​GCT​TGA​TGG​TGT​CCT​GGA​TGA​TCT​GTC Amplification of REEP4-TurboID-HA 
AB11 GGT​TTA​AAC​GGG​CCC​TCT​AGC​TAT​GCG​TAA​TCC​GGT​ACA​TCG Amplification of REEP4- and REEP5-TurboID-HA 
AB30 AGC​GTT​TAA​ACT​TAA​GCT​TGA​TGT​CTG​CGG​CCA​TGA​G Amplification of REEP5-TurboID-HA 
AB32 GGT​TTA​AAC​GGG​CCC​TCT​AGC​CTC​TAG​ATG​CAT​GCT​CGA​GTC Amplification of HA-TurboID-3xNLS 
AB33 AGC​GTT​TAA​ACT​TAA​GCT​TGT​ACC​CCT​ATG​ACG​TCC​CAG​ACT​ACG​CAA​AAG​ACA​ATA​C Amplification of HA-TurboID-3xNLS 
NameSequence (5′ to 3′)Purpose
M1-REEP4 CTC​ATG​CTG​TGC​CTC​CCC​TTT​CCC​CAG​GGC​ACC​TCG​CGC​TCC​CTG​AAG​GTT​CGG​ACG​AGG​AAA​AAG​ACT​GTG​CCC​TCA​GAC​GTG​GAC​AGC​TCA​GGT​GGA​GGA​GGT​AGT​G Generation of amplicon for HA-tagging of REEP4 at the endogenous locus with CRISPR/Cas12 (Fueller et al., 2020) 
M2-REEP4 ATA​GCC​CTG​GAG​CCC​TGC​AGG​GCA​CGA​GGT​AAG​AAG​GGG​GCA​GAT​GCA​GCA​GAC​CAA​AAA​ATG​GAC​AGC​TAG​GGT​CTG​CTG​ATC​TAC​AAG​AGT​AGA​AAT​TAG​CTA​GCT​GCA​TCG​GTA​CC Generation of amplicon for HA-tagging of REEP4 at the endogenous locus with CRISPR/Cas12 (Fueller et al., 2020) 
RGP09 AGA​TAC​TGA​GGC​AGT​CCC​CC Forward primer for sequencing of gene-edited REEP4 locus 
RGP10 GAC​TTT​CCA​CAC​CGT​CGA​CAT Reverse primer for sequencing of gene-edited REEP4 locus 
oAS363 GCC​CTG​AAA​ATA​AAG​ATT​CTC​TGT​TGT​GGG​TCC​ACT​ACC​AGA​TCC​GGA​GCT​GTC​CAC​GTC​TGA​GGG​CAC Generation of REEP4-V5-tev-HA (see text) 
oAS365 TCT​TTA​TTT​TCA​GGG​CAG​TGG​TAG​TGG​CAG​CGG​ATA​CCC​ATA​CGA​TGT​TCC​AGA​TTA​C Generation of REEP4-V5-tev-HA (see text) 
oAS416 TCC​GAG​CAG​GGG​ATT​GGG​GAT​AGG​CTT​GCC​TCC​ACT​ACC​AGA​TCC​GGA​GC Generation of REEP4-V5-tev-HA (see text) 
oAS417 AAT​CCC​CTG​CTC​GGA​CTG​GAT​AGC​ACC​CCC​ACA​ACA​GAG​AAT​CTT​TAT​TTT​C Generation of REEP4-V5-tev-HA (see text) 
oAS421 TCC​GGA​TCT​GGT​AGT​GGA​GGC​AAG​CCT​ATC​CCC​AAT​CCC​CTG​CTC​GGA​CTG​GAT​AGC​ACC​CCC​ACA​ACA​GAG​AAT​CTT​TAT​TTT​CAG​GGC Generation of REEP3-V5-tev-HA (see text) 
oAS422 GCC​CTG​AAA​ATA​AAG​ATT​CTC​TGT​TGT​GGG​GGT​GCT​ATC​CAG​TCC​GAG​CAG​GGG​ATT​GGG​GAT​AGG​CTT​GCC​TCC​ACT​ACC​AGA​TCC​GGA Generation of REEP3-V5-tev-HA (see text) 
oAS423 CAC​TAC​CAG​ATC​CGG​AAA​AAT​ACA​CTT​GTG​GTC​GTT​TCT​TC Generation of REEP3-V5-tev-HA (see text) 
oAS424 AAT​CTT​TAT​TTT​CAG​GGC​AGT​GGT​AGT​GGC​AGC​GGA​TAC​CCA​TAC​GAT​GTT​CCA​GAT​TAC Generation of REEP3-V5-tev-HA (see text) 
oAS492 AGT​GGT​AGT​GGC​AGC​GGA​CCC​ACA​ACA​GAG​AAT​CTT​TAT​TTT​CAG​GGC​GGC​AAG​CCT​ATC​CCC​AAT​CCC​CTG​CTC​GGA​CTG​GAT​AGC​ACC​TCC​GGA​TCT​GGT​AGT​GGA Generation of HA-tev-V5-Lap2β (see text) 
oAS493 TCC​ACT​ACC​AGA​TCC​GGA​GGT​GCT​ATC​CAG​TCC​GAG​CAG​GGG​ATT​GGG​GAT​AGG​CTT​GCC​GCC​CTG​AAA​ATA​AAG​ATT​CTC​TGT​TGT​GGG​TCC​GCT​GCC​ACT​ACC​ACT Generation of HA-tev-V5-Lap2β (see text) 
oAS494 GCT​GCC​ACT​ACC​ACT​AGC​GTA​ATC​TGG​AAC​ATC​GTA​TGG​GTA​CAT​GGT​GGC​GAC​CGG​TAG​C Generation of HA-tev-V5-Lap2β (see text) 
oAS495 CGG​ATC​TGG​TAG​TGG​AAT​GCC​GGA​GTT​CCT​AGA​GGA​C Generation of HA-tev-V5-Lap2β (see text) 
AB20 GGC​AGC​GGA​TCC​GGG​TCT​GGC​TCC​GGA​TCA​AAA​GAC​AAT​ACT​GTG​CCT​CTG​AAG​C Amplification of TurboID sequence 
AB21 CTA​TGC​GTA​ATC​CGG​TAC​ATC​GTA​AGG​GTA​GCT​AGC​CTT​TTC​GGC​AGA​C Amplification of TurboID sequence 
AB03 ATG​TAC​CGG​ATT​ACG​CAT​AGT​GTA​CAA​GTA​AAG​CGG​CCG​C Amplification/linearization of REEP4-HA–containing vector 
AB15 CCA​GAC​CCG​GAT​CCG​CTG​CCG​CTG​TCC​ACG​TCT​GAG​GG Amplification/linearization of REEP4-HA–containing vector 
AB22 CGG​ACT​CAG​ATC​TCG​AGC​TCA​TGT​CTG​CGG​CCA​TGA​G Amplification of REEP5 sequence 
AB23 CCA​GAC​CCG​GAT​CCG​CTG​CCG​GTG​CTC​TTC​TTT​TCT​TCA​CCC Amplification of REEP5 sequence 
AB24 ATG​TAC​CGG​ATT​ACG​CAT​AGT​GTA​CAA​GTA​AAG​CGG​CCG​C Amplification of REEP4-HA vector backbone (excluding REEP4-HA) 
AB25 GAG​CTC​GAG​ATC​TGA​GTC​CG Amplification of REEP4-HA vector backbone (excluding REEP4-HA) 
AB26 AGA​AAT​CAG​TCT​GCG​GTC​TGC Amplification of vector backbone (from 3xHA-miniturbo-NLS; Addgene) 
AB27 TGCGTAGTCTGGGACGTC Amplification of vector backbone (from 3xHA-miniturbo-NLS; Addgene) 
AB28 ATG​ACG​TCC​CAG​ACT​ACG​CAA​AAG​ACA​ATA​CTG​TGC​CTC​TGA​AGC Amplification of TurboID-NLS 
AB29 CTTTTCGGCAGACCGCAG Amplification of TurboID-NLS 
AB08 CTA​GAG​GGC​CCG​TTT​AAA​CC Amplification/linearization of pcDNA5/FRT/TO vector 
AB09 CAA​GCT​TAA​GTT​TAA​ACG​CTA​GAG​TCC Amplification/linearization of pcDNA5/FRT/TO vector 
AB10 AGC​GTT​TAA​ACT​TAA​GCT​TGA​TGG​TGT​CCT​GGA​TGA​TCT​GTC Amplification of REEP4-TurboID-HA 
AB11 GGT​TTA​AAC​GGG​CCC​TCT​AGC​TAT​GCG​TAA​TCC​GGT​ACA​TCG Amplification of REEP4- and REEP5-TurboID-HA 
AB30 AGC​GTT​TAA​ACT​TAA​GCT​TGA​TGT​CTG​CGG​CCA​TGA​G Amplification of REEP5-TurboID-HA 
AB32 GGT​TTA​AAC​GGG​CCC​TCT​AGC​CTC​TAG​ATG​CAT​GCT​CGA​GTC Amplification of HA-TurboID-3xNLS 
AB33 AGC​GTT​TAA​ACT​TAA​GCT​TGT​ACC​CCT​ATG​ACG​TCC​CAG​ACT​ACG​CAA​AAG​ACA​ATA​C Amplification of HA-TurboID-3xNLS 
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