Table 1.
Used primer sequences of this study
ConstructPrimersSequence (5′→3′)
RIM-BPFL Fwd CAC​CAT​GCA​TTT​ATG​TGA​ATT​TCC​CAG​CGC 
Rev GTA​TTT​GCC​AAA​GCC​GAA​CCC​GAA​TTT​TC 
RIM-BPΔNTR1(26-250) Fwd_1 GAT​CGG​CGC​GCC​ATG​CAT​TTA​TGT​GAA​TTT​CCC​AGC​GC 
Rev_1 CGC​TTC​CTC​CCA​GAA​CTG​CTC​TTT​TTG​CTG​CTG​CTG​CTG​CTT​TTT​CAG​G 
Fwd_2 CCT​GAA​AAA​GCA​GCA​GCA​GCA​GCA​AAA​AGA​GCA​GTT​CTG​GGA​GGA​AGC​GG 
Rev_2 GAT​CGA​GCT​CGC​CCT​CAG​CCT​CCC​TGC​AAG​G 
RIM-BPΔNTR2(152-599) Fwd_3 ATC​CCG​CTC​GGA​TAT​TCC​TGG​AAA​GGG​CCG 
Rev_3 CAG​GAA​TAT​CCG​AGC​GGG​ATG​CCT​G 
RIM-BPΔSH3I(606-681) Fwd_4 TGG​AAA​GGG​CGA​CCT​CCT​GGA​GTT​CCA​CC 
Rev_4 CCA​GGA​GGT​CGC​CCT​TTC​CAG​GAA​TAT​CGA​G 
RIM-BPΔSH3II(1318-1382) Fwd_5 GAA​GAA​GCC​CGA​CAC​CAC​CGC​CTC​C 
Rev_5 TGT​ACT​GGT​CGG​GCT​TCT​TCT​GTG​CTC​CGG​G 
RIM-BPΔSH3III(1445-1509) Fwd_6 CAT​GCC​CGT​GGC​GCC​CGA​CCA​GTA​CAA​C 
Rev_6 GCG​CCC​GAC​CAG​CAC​GGG​CAT​GTT​GGC 
RIM-BPΔFN3 I(747-826) Fwd_7 CAG​CGA​CAA​TCG​GGA​TGC​CGC​CTG​C 
Rev_7 CGG​GAT​GCC​GAT​TGT​CGC​TGA​TGT​CG 
RIM-BPΔFN3 II(837-938) Fwd_8 GAT​CAT​CGG​GGA​CTT​CCG​CAC​CCT​CAC​CAA​G 
Rev_8 GGC​CCT​TGG​TCC​CGA​TGA​TCA​TCG​TGC 
RIM-BPΔFN3 III(951-1030) Fwd_9 CAA​GGG​CCT​GGC​GGA​CAG​TGC​GC 
Rev_9 GCG​GAC​AGT​GCA​GGC​CCT​TGG 
RIM-BPSH3 II + III OE(1317-1509) Fwd_10 GAA​GAA​GCC​CGA​CCA​GTA​CAA​CAA​CCA​GAT​GG 
Rev_10 TGT​ACT​GGT​CGG​GCT​TCT​TCT​GTG​CTC​CGG​G 
ConstructPrimersSequence (5′→3′)
RIM-BPFL Fwd CAC​CAT​GCA​TTT​ATG​TGA​ATT​TCC​CAG​CGC 
Rev GTA​TTT​GCC​AAA​GCC​GAA​CCC​GAA​TTT​TC 
RIM-BPΔNTR1(26-250) Fwd_1 GAT​CGG​CGC​GCC​ATG​CAT​TTA​TGT​GAA​TTT​CCC​AGC​GC 
Rev_1 CGC​TTC​CTC​CCA​GAA​CTG​CTC​TTT​TTG​CTG​CTG​CTG​CTG​CTT​TTT​CAG​G 
Fwd_2 CCT​GAA​AAA​GCA​GCA​GCA​GCA​GCA​AAA​AGA​GCA​GTT​CTG​GGA​GGA​AGC​GG 
Rev_2 GAT​CGA​GCT​CGC​CCT​CAG​CCT​CCC​TGC​AAG​G 
RIM-BPΔNTR2(152-599) Fwd_3 ATC​CCG​CTC​GGA​TAT​TCC​TGG​AAA​GGG​CCG 
Rev_3 CAG​GAA​TAT​CCG​AGC​GGG​ATG​CCT​G 
RIM-BPΔSH3I(606-681) Fwd_4 TGG​AAA​GGG​CGA​CCT​CCT​GGA​GTT​CCA​CC 
Rev_4 CCA​GGA​GGT​CGC​CCT​TTC​CAG​GAA​TAT​CGA​G 
RIM-BPΔSH3II(1318-1382) Fwd_5 GAA​GAA​GCC​CGA​CAC​CAC​CGC​CTC​C 
Rev_5 TGT​ACT​GGT​CGG​GCT​TCT​TCT​GTG​CTC​CGG​G 
RIM-BPΔSH3III(1445-1509) Fwd_6 CAT​GCC​CGT​GGC​GCC​CGA​CCA​GTA​CAA​C 
Rev_6 GCG​CCC​GAC​CAG​CAC​GGG​CAT​GTT​GGC 
RIM-BPΔFN3 I(747-826) Fwd_7 CAG​CGA​CAA​TCG​GGA​TGC​CGC​CTG​C 
Rev_7 CGG​GAT​GCC​GAT​TGT​CGC​TGA​TGT​CG 
RIM-BPΔFN3 II(837-938) Fwd_8 GAT​CAT​CGG​GGA​CTT​CCG​CAC​CCT​CAC​CAA​G 
Rev_8 GGC​CCT​TGG​TCC​CGA​TGA​TCA​TCG​TGC 
RIM-BPΔFN3 III(951-1030) Fwd_9 CAA​GGG​CCT​GGC​GGA​CAG​TGC​GC 
Rev_9 GCG​GAC​AGT​GCA​GGC​CCT​TGG 
RIM-BPSH3 II + III OE(1317-1509) Fwd_10 GAA​GAA​GCC​CGA​CCA​GTA​CAA​CAA​CCA​GAT​GG 
Rev_10 TGT​ACT​GGT​CGG​GCT​TCT​TCT​GTG​CTC​CGG​G 
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