Table 1.
Lentiviral plasmids encoding desired shRNA for stable protein knock-down
VectorshRNAPrimer
pLKO.1 hANKRD28 hANKRD28_shRNA_sense 
5′-ccg​gaa​GCC​TTA​GGT​CCT​ATT​CAT​Act​cga​gTA​TGA​ATA​GGA​CCT​AAG​GCt​ttt​ttt​g-3′ 
hANKRD28_shRNA_anti 
5′-aat​tca​aaa​aaa​GCC​TTA​GGT​CCT​ATT​CAT​Act​cga​gTA​TGA​ATA​GGA​CCT​AAG​GCt​t-3′ 
pLKO.1 hβ1 Integrin hβ1 Integrin_shRNA_sense 
5′-ccg​gaa​TGC​AGC​ACA​GAT​GAA​GTT​Act​cga​gTA​ACT​TCA​TCT​GTG​CTG​CAt​ttt​ttt​g-3′ 
hβ1 Integrin_shRNA_anti 
5′-aat​tca​aaa​aaa​TGC​AGC​ACA​GAT​GAA​GTT​Act​cga​gTA​ACT​TCA​TCT​GTG​CTG​CAt​t-3′ 
pLKO.1 hFilaminA hFilaminA_shRNA_sense 
5′-ccg​gaa​GAC​CAC​CTA​CTT​TGA​GAT​Cct​cga​gGA​TCT​CAA​AGT​AGG​TGG​TCt​ttt​ttt​g-3′ 
hFilaminA_shRNA_anti 
5′-aat​tca​aaa​aaa​GAC​CAC​CTA​CTT​TGA​GAT​Cct​cga​gGA​TCT​CAA​AGT​AGG​TGG​TCt​t-3′ 
pLKO.1 hILKAP hILKAP_shRNA_sense 
5′-ccg​gaa​AGA​AAG​TTT​GTA​AAG​CCT​Cct​cga​gGA​GGC​TTT​ACA​AAC​TTT​CTt​ttt​ttt​g-3′ 
hILKAP_shRNA_anti 
5′-aat​tca​aaa​aaa​AGA​AAG​TTT​GTA​AAG​CCT​Cct​cga​gGA​GGC​TTT​ACA​AAC​TTT​CTt​t-3′ 
pLKO.1 hPPM1F hPPM1F_shRNA_sense 
5′-ccg​gaa​CCA​GCT​CTT​CGG​CTT​GTC​Tct​cga​gAG​ACA​AGC​CGA​AGA​GCT​GGt​ttt​ttt​g-3′ 
hPPM1F_shRNA_anti 
5′-aat​tca​aaa​aaa​CCA​GCT​CTT​CGG​CTT​GTC​Tct​cga​gAG​ACA​AGC​CGA​AGA​GCT​GGt​t-3′ 
pLKO.1 hPP2A hPP2A_shRNA_sense 
5′-ccg​gaa​TGG​GAA​GAG​CAA​CAG​TAA​Cct​cga​gGT​TAC​TGT​TGC​TCT​TCC​CAt​ttt​ttt​g-3′ 
hPP2A_shRNA_anti 
5′-aat​tca​aaa​aaa​TGG​GAA​GAG​CAA​CAG​TAA​Cct​cga​gGT​TAC​TGT​TGC​TCT​TCC​CAt​t-3′ 
pLKO.1 hPTP1B hPTP1B_shRNA_sense 
5′-ccg​gaa​CTC​TCC​ACT​CCA​TAT​TTA​Tct​cga​gAT​AAA​TAT​GGA​GTG​GAG​AGt​ttt​ttt​g-3′ 
hPTP1B_shRNA_anti 
5′-aat​tca​aaa​aaa​CTC​TCC​ACT​CCA​TAT​TTA​Tct​cga​gAT​AAA​TAT​GGA​GTG​GAG​AGt​t-3′ 
pLKO.1 hPTP-PEST hPTP_PEST_shRNA_sense 
5′-ccg​gaa​GCC​AGA​TTT​ATA​GTA​TTC​Cct​cga​gGG​AAT​ACT​ATA​AAT​CTG​GCt​ttt​ttt​g-3′ 
hPTP-PEST_shRNA_anti 
5′-aat​tca​aaa​aaa​GCC​AGA​TTT​ATA​GTA​TTC​Cct​cga​gGG​AAT​ACT​ATA​AAT​CTG​GCt​t-3′ 
pLKO.1 hPTPRF hPTPRF_shRNA_sense 
5′-ccg​gaa​TCA​GAG​AGC​CTA​GAA​CAT​Cct​cga​gGA​TGT​TCT​AGG​CTC​TCT​GAt​ttt​ttt​g-3′ 
hPTPRF_shRNA_anti 
5′-aat​tca​aaa​aaa​TCA​GAG​AGC​CTA​GAA​CAT​Cct​cga​gGA​TGT​TCT​AGG​CTC​TCT​GAt​t-3′ 
pLKO.1 hPTPRO hPTPRO_shRNA_sense 
5′-ccg​gaa​GAA​ATG​GTC​ATT​CTA​CTT​Cct​cga​gGA​AGT​AGA​ATG​ACC​ATT​TCt​ttt​ttt​g-3′ 
hPTPRO_shRNA_anti 
5′-aat​tca​aaa​aaa​GAA​ATG​GTC​ATT​CTA​CTT​Cct​cga​gGA​AGT​AGA​ATG​ACC​ATT​TCt​t-3′ 
pLKO.1 hRPTPα hRPTPα_shRNA_sense 
5′-ccg​gaa​TGG​ATG​ATG​CAG​TTC​AAA​Tct​cga​gAT​TTG​AAC​TGC​ATC​ATC​CAt​ttt​ttt​g-3′ 
hRPTPα_shRNA_anti 
5′-aat​tca​aaa​aaa​TGG​ATG​ATG​CAG​TTC​AAA​Tct​cga​gAT​TTG​AAC​TGC​ATC​ATC​CAt​t-3′ 
pLKO.1 hSHP1 hSHP1_shRNA_sense 
5′-ccg​gaa​CCC​TTC​TCC​TCT​TGT​AAA​Tct​cga​gAT​TTA​CAA​GAG​GAG​AAG​GGt​ttt​ttt​g-3′ 
hSHP1_shRNA_anti 
5′-aat​tca​aaa​aaa​CCC​TTC​TCC​TCT​TGT​AAA​Tct​cga​gAT​TTA​CAA​GAG​GAG​AAG​GGt​t-3′ 
pLKO.1 hSHP2 hSHP2_shRNA_sense 
5′-ccg​gaa​CAG​ACG​CAA​GAA​AGT​TTA​Tct​cga​gAT​AAA​CTT​TCT​TGC​GTC​TGt​ttt​ttt​g-3′ 
hSHP2_shRNA_anti 
5′-aat​tca​aaa​aaa​CAG​ACG​CAA​GAA​AGT​TTA​Tct​cga​gAT​AAA​CTT​TCT​TGC​GTC​TGt​t-3′ 
pLKO.1 hTCPTP hTCPTP_shRNA_sense 
5′-ccg​gaa​CCT​GCA​CTT​GAT​ATA​AGC​Act​cga​gTG​CTT​ATA​TCA​AGT​GCA​GGt​ttt​ttt​g-3′ 
hTCPTP_shRNA_anti 
5′-aat​tca​aaa​aaa​CCT​GCA​CTT​GAT​ATA​AGC​Act​cga​gTG​CTT​ATA​TCA​AGT​GCA​GGt​t-3′ 
VectorshRNAPrimer
pLKO.1 hANKRD28 hANKRD28_shRNA_sense 
5′-ccg​gaa​GCC​TTA​GGT​CCT​ATT​CAT​Act​cga​gTA​TGA​ATA​GGA​CCT​AAG​GCt​ttt​ttt​g-3′ 
hANKRD28_shRNA_anti 
5′-aat​tca​aaa​aaa​GCC​TTA​GGT​CCT​ATT​CAT​Act​cga​gTA​TGA​ATA​GGA​CCT​AAG​GCt​t-3′ 
pLKO.1 hβ1 Integrin hβ1 Integrin_shRNA_sense 
5′-ccg​gaa​TGC​AGC​ACA​GAT​GAA​GTT​Act​cga​gTA​ACT​TCA​TCT​GTG​CTG​CAt​ttt​ttt​g-3′ 
hβ1 Integrin_shRNA_anti 
5′-aat​tca​aaa​aaa​TGC​AGC​ACA​GAT​GAA​GTT​Act​cga​gTA​ACT​TCA​TCT​GTG​CTG​CAt​t-3′ 
pLKO.1 hFilaminA hFilaminA_shRNA_sense 
5′-ccg​gaa​GAC​CAC​CTA​CTT​TGA​GAT​Cct​cga​gGA​TCT​CAA​AGT​AGG​TGG​TCt​ttt​ttt​g-3′ 
hFilaminA_shRNA_anti 
5′-aat​tca​aaa​aaa​GAC​CAC​CTA​CTT​TGA​GAT​Cct​cga​gGA​TCT​CAA​AGT​AGG​TGG​TCt​t-3′ 
pLKO.1 hILKAP hILKAP_shRNA_sense 
5′-ccg​gaa​AGA​AAG​TTT​GTA​AAG​CCT​Cct​cga​gGA​GGC​TTT​ACA​AAC​TTT​CTt​ttt​ttt​g-3′ 
hILKAP_shRNA_anti 
5′-aat​tca​aaa​aaa​AGA​AAG​TTT​GTA​AAG​CCT​Cct​cga​gGA​GGC​TTT​ACA​AAC​TTT​CTt​t-3′ 
pLKO.1 hPPM1F hPPM1F_shRNA_sense 
5′-ccg​gaa​CCA​GCT​CTT​CGG​CTT​GTC​Tct​cga​gAG​ACA​AGC​CGA​AGA​GCT​GGt​ttt​ttt​g-3′ 
hPPM1F_shRNA_anti 
5′-aat​tca​aaa​aaa​CCA​GCT​CTT​CGG​CTT​GTC​Tct​cga​gAG​ACA​AGC​CGA​AGA​GCT​GGt​t-3′ 
pLKO.1 hPP2A hPP2A_shRNA_sense 
5′-ccg​gaa​TGG​GAA​GAG​CAA​CAG​TAA​Cct​cga​gGT​TAC​TGT​TGC​TCT​TCC​CAt​ttt​ttt​g-3′ 
hPP2A_shRNA_anti 
5′-aat​tca​aaa​aaa​TGG​GAA​GAG​CAA​CAG​TAA​Cct​cga​gGT​TAC​TGT​TGC​TCT​TCC​CAt​t-3′ 
pLKO.1 hPTP1B hPTP1B_shRNA_sense 
5′-ccg​gaa​CTC​TCC​ACT​CCA​TAT​TTA​Tct​cga​gAT​AAA​TAT​GGA​GTG​GAG​AGt​ttt​ttt​g-3′ 
hPTP1B_shRNA_anti 
5′-aat​tca​aaa​aaa​CTC​TCC​ACT​CCA​TAT​TTA​Tct​cga​gAT​AAA​TAT​GGA​GTG​GAG​AGt​t-3′ 
pLKO.1 hPTP-PEST hPTP_PEST_shRNA_sense 
5′-ccg​gaa​GCC​AGA​TTT​ATA​GTA​TTC​Cct​cga​gGG​AAT​ACT​ATA​AAT​CTG​GCt​ttt​ttt​g-3′ 
hPTP-PEST_shRNA_anti 
5′-aat​tca​aaa​aaa​GCC​AGA​TTT​ATA​GTA​TTC​Cct​cga​gGG​AAT​ACT​ATA​AAT​CTG​GCt​t-3′ 
pLKO.1 hPTPRF hPTPRF_shRNA_sense 
5′-ccg​gaa​TCA​GAG​AGC​CTA​GAA​CAT​Cct​cga​gGA​TGT​TCT​AGG​CTC​TCT​GAt​ttt​ttt​g-3′ 
hPTPRF_shRNA_anti 
5′-aat​tca​aaa​aaa​TCA​GAG​AGC​CTA​GAA​CAT​Cct​cga​gGA​TGT​TCT​AGG​CTC​TCT​GAt​t-3′ 
pLKO.1 hPTPRO hPTPRO_shRNA_sense 
5′-ccg​gaa​GAA​ATG​GTC​ATT​CTA​CTT​Cct​cga​gGA​AGT​AGA​ATG​ACC​ATT​TCt​ttt​ttt​g-3′ 
hPTPRO_shRNA_anti 
5′-aat​tca​aaa​aaa​GAA​ATG​GTC​ATT​CTA​CTT​Cct​cga​gGA​AGT​AGA​ATG​ACC​ATT​TCt​t-3′ 
pLKO.1 hRPTPα hRPTPα_shRNA_sense 
5′-ccg​gaa​TGG​ATG​ATG​CAG​TTC​AAA​Tct​cga​gAT​TTG​AAC​TGC​ATC​ATC​CAt​ttt​ttt​g-3′ 
hRPTPα_shRNA_anti 
5′-aat​tca​aaa​aaa​TGG​ATG​ATG​CAG​TTC​AAA​Tct​cga​gAT​TTG​AAC​TGC​ATC​ATC​CAt​t-3′ 
pLKO.1 hSHP1 hSHP1_shRNA_sense 
5′-ccg​gaa​CCC​TTC​TCC​TCT​TGT​AAA​Tct​cga​gAT​TTA​CAA​GAG​GAG​AAG​GGt​ttt​ttt​g-3′ 
hSHP1_shRNA_anti 
5′-aat​tca​aaa​aaa​CCC​TTC​TCC​TCT​TGT​AAA​Tct​cga​gAT​TTA​CAA​GAG​GAG​AAG​GGt​t-3′ 
pLKO.1 hSHP2 hSHP2_shRNA_sense 
5′-ccg​gaa​CAG​ACG​CAA​GAA​AGT​TTA​Tct​cga​gAT​AAA​CTT​TCT​TGC​GTC​TGt​ttt​ttt​g-3′ 
hSHP2_shRNA_anti 
5′-aat​tca​aaa​aaa​CAG​ACG​CAA​GAA​AGT​TTA​Tct​cga​gAT​AAA​CTT​TCT​TGC​GTC​TGt​t-3′ 
pLKO.1 hTCPTP hTCPTP_shRNA_sense 
5′-ccg​gaa​CCT​GCA​CTT​GAT​ATA​AGC​Act​cga​gTG​CTT​ATA​TCA​AGT​GCA​GGt​ttt​ttt​g-3′ 
hTCPTP_shRNA_anti 
5′-aat​tca​aaa​aaa​CCT​GCA​CTT​GAT​ATA​AGC​Act​cga​gTG​CTT​ATA​TCA​AGT​GCA​GGt​t-3′ 
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