Table 1. List of oligos Name . Sequence... | Rockefeller University Press

Table 1.

List of oligos

Name	Sequence (5′- to -3′)	Purpose	Construct
EH-524	gagctctcgagaattctcacgcgtatgcagatcccacaggcg	Clone human PD-1^{WT or FF} into pHR-mGFP vector	pHR-PD-1^{WT or FF}-mGFP
EH-863	gcaagcttgatatcctgcagacgcgtcaggggccaagagcagt	Clone human PD-1^{WT or FF} into pHR-mGFP vector	pHR-PD-1^{WT or FF}-mGFP
EH-741	gagctctcgagaattctcatgaagacattgcctgccat	Clone human BTLA^{WT or FF} into pHR-mGFP vector	pHR-BTLA^{WT or FF}-mGFP
EH-742	caagcttgatatcctgcagacgcgtactcctcacacatatggatg	Clone human BTLA^{WT or FF} into pHR-mGFP vector	pHR-BTLA^{WT or FF}-mGFP
EH-1004	ctggccgtcatctgctccagaaggcaccaaggaaagc	Pair with EH-742, clone human BTLA^INT into pHR-mGFP vector	pHR–PD-1^ECTO-TMD–BTLA^INT-mGFP
EH-1005	ggagcagatgacggccag	Pair with EH-524, clone human PD-1^ECTO-TMD into pHR-mGFP vector	pHR–PD-1^ECTO-TMD–BTLA^INT-mGFP
EH-1006	ctgcctgttctgctgcctgcgggccgcacgagggaca	Pair with EH-863, clone human PD-1^INT into pHR-mGFP vector	pHR–BTLA^ECTO-TMD–PD-1^INT–mGFP
EH-1007	caggcagcagaacaggcag	Pair with EH-741, clone human BTLA^ECTO-TMD into pHR-mGFP vector	pHR–BTLA^ECTO-TMD–PD-1^INT–mGFP
EH-1829	gagctctcgagaattctcacgcgtatgaggatatttgctgtctttatattcatg	Clone human PD-L1 into pHR-mCherry vector	pHR–PD-L1–mCherry
EH-959	caccatggtggcgaccggtggatccgtctcctccaaatgtgtatc	Clone human PD-L1 into pHR-mCherry vector	pHR–PD-L1–mCherry
EH-70	agctctcgagaattctcatggagcctcctggag	Clone human PD-L1 into pHR-mRuby vector	pHR-HVEM-mRuby
EH-71	caagcttgatatcctgcagacggtggtttgggctcctccc	Clone human PD-L1 into pHR-mRuby vector	pHR-HVEM-mRuby
EH-1391	tggaggctcgagcggtggcggccgcatggtgaggtggtttcaccg	Clone human SHP1 into pHR-mCherry vector	pHR-mCherry-SHP1
EH-1392	cgactctagagtcgcggccgctcacttcctcttgagggaacc	Clone human SHP1 into pHR-mCherry vector	pHR-mCherry-SHP1
EH-1078	tggaggctcgagcggtggcggccgcatgacatcgcggagatgg	Clone human SHP2 into pHR-mCherry vector	pHR-mCherry-SHP2
EH-1079	tctagagtcgcggccgcttcatctgaaacttttctgctgttg	Clone human SHP2 into pHR-mCherry vector	pHR-mCherry-SHP2
EH-396	tctgttccaggggcccctgggatcccgggccgcacgagg	Clone human PD-1^{INT (WT or FF)} into pGEX6p2-GST vector	pGEX6p2-GST–PD-1^{INT (WT or FF)}
EH-397	ggccgctcgagtcgacccgggttacaggggccaagagc	Clone human PD-1^{INT (WT or FF)} into pGEX6p2-GST vector	pGEX6p2-GST–PD-1^{INT (WT or FF)}
EH-637	ttaactttaagaaggagatataccatgtcccctatactaggt	Clone GST-human BTLA^{INT (WT or FFFF)} into pET28A-TwinStrep vector	pET28A-GST-BTLA^{INT (WT or FFFF)}-TwinStrep
EH-607	tccacctttctcgaactgcgggtggctccaggatccactcctcacacatatgg		pET28A-GST-BTLA^{INT (WT or FFFF)}-TwinStrep
EH-1070	gagctctcgagaattctcacgcgtatgtgggtccggcag	Clone mouse PD-1 into pMIG II-mGFP vector	pMIG II-mouse PD-1–mGFP
EH-1071	agcttgatatcctgcagacgcgtaagaggccaagaacaatgtc	Clone mouse PD-1 into pMIG II-mGFP vector	pMIG II-mouse PD-1–mGFP
EH-1376	gagctctcgagaattctcacgcgtatgaagacagtgcctgccatg	Clone mouse BTLA into pMIG II-mGFP vector	pMIG II-mouse BTLA-mGFP
EH-1377	caagcttgatatcctgcagacgcgtacttctcacacaaatggatgc	Clone mouse BTLA into pMIG II-mGFP vector	pMIG II-mouse BTLA-mGFP
EH-1386	tggaggctcgagcggtggcggccgcatggtgaggtggtttcaccgggacc	Clone mouse SHP1 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP1
EH-1387	tggaggctcgagcggtggcggccgcatgacatcgcggagatgg	Clone mouse SHP1 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP1
EH-1389	cgactctagagtcgcggccgctcacttcctcttgagagaacctttg	Clone mouse SHP2 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP2
EH-1390	cgactctagagtcgcggccgctcatctgaaactcctctgctg	Clone mouse SHP2 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP2
EH-899	caccgtcggcccagtcgcaagaacc	SHP1_gRNA1	pX330-GFP SHP1_gRNA1
EH-900	aaacggttcttgcgactgggccga	SHP1_gRNA1	pX330-GFP SHP1_gRNA1
EH-901	caccggacacctcggcccttgagc	SHP1_gRNA2	pX330-GFP SHP1_gRNA2
EH-902	aaacgctcaagggccgaggtgtc	SHP1_gRNA2	pX330-GFP SHP1_gRNA2
EH-387	caccggagacttcacactttccgtt	SHP2_gRNA1	pX330-GFP SHP2_gRNA1
EH-388	aaacaacggaaagtgtgaagtctcc	SHP2_gRNA1	pX330-GFP SHP2_gRNA1
EH-389	caccgtacagtactacaactcaagc	SHP2_gRNA2	pX330-GFP SHP2_gRNA2
EH-390	aaacgcttgagttgtagtactgtac	SHP2_gRNA2	pX330-GFP SHP2_gRNA2
EH-26	tggacagcaaatgggtcgcggacgggccgcacgaggga	Clone human PD-1^INT into pET28A-His10 vector	pET28A-His₁₀–PD-1^INT
EH-1949	gagctcgaattcgtcacaggggccaagagc	Clone human PD-1^INT into pET28A-His10 vector	pET28A-His₁₀–PD-1^INT
EH-1498	cagcaaatgggtcgcggaggcttctgggaggagttt	Clone human SHP1^PTPase into pET28A-His₁₀ vector	pET28A-His₁₀-SHP1^PTPase
EH-1499	gacggagctcgaattcttaataggtgatgttcccgtact	Clone human SHP1^PTPase into pET28A-His₁₀ vector	pET28A-His₁₀-SHP1^PTPase
EH-1502	ggacagcaaatgggtcgcggatccggcttttgggaggaatttg	Clone human SHP2^PTPase into pET28A-His₁₀ vector	pET28aA-His₁₀-SHP2^PTPase
EH-1538	gtcgacggagctcgaattctcaatacttaatatttgtatattcgtgcc	Clone human SHP2^PTPase into pET28A-His₁₀ vector	pET28aA-His₁₀-SHP2^PTPase
EH-606	ctttaagaaggagatataccatgggcagcagccatcat	Clone His₁₀-human BTLA^{INT (WT, YFFF, FYFF, FFYF, or FFFY)} into pET28A-TwinStrep vector	pET28A-His₁₀-BTLA^{INT (WT, YFFF, FYFF, FFYF or FFFY)}-TwinStrep
EH-607	tccacctttctcgaactgcgggtggctccaggatccactcctcacacatatgg

Name	Sequence (5′- to -3′)	Purpose	Construct
EH-524	gagctctcgagaattctcacgcgtatgcagatcccacaggcg	Clone human PD-1^{WT or FF} into pHR-mGFP vector	pHR-PD-1^{WT or FF}-mGFP
EH-863	gcaagcttgatatcctgcagacgcgtcaggggccaagagcagt	Clone human PD-1^{WT or FF} into pHR-mGFP vector	pHR-PD-1^{WT or FF}-mGFP
EH-741	gagctctcgagaattctcatgaagacattgcctgccat	Clone human BTLA^{WT or FF} into pHR-mGFP vector	pHR-BTLA^{WT or FF}-mGFP
EH-742	caagcttgatatcctgcagacgcgtactcctcacacatatggatg	Clone human BTLA^{WT or FF} into pHR-mGFP vector	pHR-BTLA^{WT or FF}-mGFP
EH-1004	ctggccgtcatctgctccagaaggcaccaaggaaagc	Pair with EH-742, clone human BTLA^INT into pHR-mGFP vector	pHR–PD-1^ECTO-TMD–BTLA^INT-mGFP
EH-1005	ggagcagatgacggccag	Pair with EH-524, clone human PD-1^ECTO-TMD into pHR-mGFP vector	pHR–PD-1^ECTO-TMD–BTLA^INT-mGFP
EH-1006	ctgcctgttctgctgcctgcgggccgcacgagggaca	Pair with EH-863, clone human PD-1^INT into pHR-mGFP vector	pHR–BTLA^ECTO-TMD–PD-1^INT–mGFP
EH-1007	caggcagcagaacaggcag	Pair with EH-741, clone human BTLA^ECTO-TMD into pHR-mGFP vector	pHR–BTLA^ECTO-TMD–PD-1^INT–mGFP
EH-1829	gagctctcgagaattctcacgcgtatgaggatatttgctgtctttatattcatg	Clone human PD-L1 into pHR-mCherry vector	pHR–PD-L1–mCherry
EH-959	caccatggtggcgaccggtggatccgtctcctccaaatgtgtatc	Clone human PD-L1 into pHR-mCherry vector	pHR–PD-L1–mCherry
EH-70	agctctcgagaattctcatggagcctcctggag	Clone human PD-L1 into pHR-mRuby vector	pHR-HVEM-mRuby
EH-71	caagcttgatatcctgcagacggtggtttgggctcctccc	Clone human PD-L1 into pHR-mRuby vector	pHR-HVEM-mRuby
EH-1391	tggaggctcgagcggtggcggccgcatggtgaggtggtttcaccg	Clone human SHP1 into pHR-mCherry vector	pHR-mCherry-SHP1
EH-1392	cgactctagagtcgcggccgctcacttcctcttgagggaacc	Clone human SHP1 into pHR-mCherry vector	pHR-mCherry-SHP1
EH-1078	tggaggctcgagcggtggcggccgcatgacatcgcggagatgg	Clone human SHP2 into pHR-mCherry vector	pHR-mCherry-SHP2
EH-1079	tctagagtcgcggccgcttcatctgaaacttttctgctgttg	Clone human SHP2 into pHR-mCherry vector	pHR-mCherry-SHP2
EH-396	tctgttccaggggcccctgggatcccgggccgcacgagg	Clone human PD-1^{INT (WT or FF)} into pGEX6p2-GST vector	pGEX6p2-GST–PD-1^{INT (WT or FF)}
EH-397	ggccgctcgagtcgacccgggttacaggggccaagagc	Clone human PD-1^{INT (WT or FF)} into pGEX6p2-GST vector	pGEX6p2-GST–PD-1^{INT (WT or FF)}
EH-637	ttaactttaagaaggagatataccatgtcccctatactaggt	Clone GST-human BTLA^{INT (WT or FFFF)} into pET28A-TwinStrep vector	pET28A-GST-BTLA^{INT (WT or FFFF)}-TwinStrep
EH-607	tccacctttctcgaactgcgggtggctccaggatccactcctcacacatatgg		pET28A-GST-BTLA^{INT (WT or FFFF)}-TwinStrep
EH-1070	gagctctcgagaattctcacgcgtatgtgggtccggcag	Clone mouse PD-1 into pMIG II-mGFP vector	pMIG II-mouse PD-1–mGFP
EH-1071	agcttgatatcctgcagacgcgtaagaggccaagaacaatgtc	Clone mouse PD-1 into pMIG II-mGFP vector	pMIG II-mouse PD-1–mGFP
EH-1376	gagctctcgagaattctcacgcgtatgaagacagtgcctgccatg	Clone mouse BTLA into pMIG II-mGFP vector	pMIG II-mouse BTLA-mGFP
EH-1377	caagcttgatatcctgcagacgcgtacttctcacacaaatggatgc	Clone mouse BTLA into pMIG II-mGFP vector	pMIG II-mouse BTLA-mGFP
EH-1386	tggaggctcgagcggtggcggccgcatggtgaggtggtttcaccgggacc	Clone mouse SHP1 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP1
EH-1387	tggaggctcgagcggtggcggccgcatgacatcgcggagatgg	Clone mouse SHP1 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP1
EH-1389	cgactctagagtcgcggccgctcacttcctcttgagagaacctttg	Clone mouse SHP2 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP2
EH-1390	cgactctagagtcgcggccgctcatctgaaactcctctgctg	Clone mouse SHP2 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP2
EH-899	caccgtcggcccagtcgcaagaacc	SHP1_gRNA1	pX330-GFP SHP1_gRNA1
EH-900	aaacggttcttgcgactgggccga	SHP1_gRNA1	pX330-GFP SHP1_gRNA1
EH-901	caccggacacctcggcccttgagc	SHP1_gRNA2	pX330-GFP SHP1_gRNA2
EH-902	aaacgctcaagggccgaggtgtc	SHP1_gRNA2	pX330-GFP SHP1_gRNA2
EH-387	caccggagacttcacactttccgtt	SHP2_gRNA1	pX330-GFP SHP2_gRNA1
EH-388	aaacaacggaaagtgtgaagtctcc	SHP2_gRNA1	pX330-GFP SHP2_gRNA1
EH-389	caccgtacagtactacaactcaagc	SHP2_gRNA2	pX330-GFP SHP2_gRNA2
EH-390	aaacgcttgagttgtagtactgtac	SHP2_gRNA2	pX330-GFP SHP2_gRNA2
EH-26	tggacagcaaatgggtcgcggacgggccgcacgaggga	Clone human PD-1^INT into pET28A-His10 vector	pET28A-His₁₀–PD-1^INT
EH-1949	gagctcgaattcgtcacaggggccaagagc	Clone human PD-1^INT into pET28A-His10 vector	pET28A-His₁₀–PD-1^INT
EH-1498	cagcaaatgggtcgcggaggcttctgggaggagttt	Clone human SHP1^PTPase into pET28A-His₁₀ vector	pET28A-His₁₀-SHP1^PTPase
EH-1499	gacggagctcgaattcttaataggtgatgttcccgtact	Clone human SHP1^PTPase into pET28A-His₁₀ vector	pET28A-His₁₀-SHP1^PTPase
EH-1502	ggacagcaaatgggtcgcggatccggcttttgggaggaatttg	Clone human SHP2^PTPase into pET28A-His₁₀ vector	pET28aA-His₁₀-SHP2^PTPase
EH-1538	gtcgacggagctcgaattctcaatacttaatatttgtatattcgtgcc	Clone human SHP2^PTPase into pET28A-His₁₀ vector	pET28aA-His₁₀-SHP2^PTPase
EH-606	ctttaagaaggagatataccatgggcagcagccatcat	Clone His₁₀-human BTLA^{INT (WT, YFFF, FYFF, FFYF, or FFFY)} into pET28A-TwinStrep vector	pET28A-His₁₀-BTLA^{INT (WT, YFFF, FYFF, FFYF or FFFY)}-TwinStrep
EH-607	tccacctttctcgaactgcgggtggctccaggatccactcctcacacatatgg

View Large

This Feature Is Available To Subscribers Only