| Methods . | Slow . | Intermediate . | Fast . | Very fast . |
|---|---|---|---|---|
| Sinusoidal analysis | Process A | Process B | Process C | Y∞ |
| (Tension transients) | Oscillatory work | |||
| Phase advance | Phase delay | Phase advance | ||
| Isometric step | Phase 4 | Phase 3 | Phase 2 | Phase 1 |
| (Tension transients) | Delayed tension | |||
| Exponential advance | Exponential delay | Exponential advance | ||
| Isotonic step (length transients) | Steady velocity of shortening phase | Phase 3 with the rate constant r3 | Phase 2 with the rate constant r2 | Phase 1 |
| Mechanisms in CB cycle | Step 6 (linear work production) | Step 4 (force generation, oscillatory work production) | Step 2 (CB detachment after ATP binding) |
| Methods . | Slow . | Intermediate . | Fast . | Very fast . |
|---|---|---|---|---|
| Sinusoidal analysis | Process A | Process B | Process C | Y∞ |
| (Tension transients) | Oscillatory work | |||
| Phase advance | Phase delay | Phase advance | ||
| Isometric step | Phase 4 | Phase 3 | Phase 2 | Phase 1 |
| (Tension transients) | Delayed tension | |||
| Exponential advance | Exponential delay | Exponential advance | ||
| Isotonic step (length transients) | Steady velocity of shortening phase | Phase 3 with the rate constant r3 | Phase 2 with the rate constant r2 | Phase 1 |
| Mechanisms in CB cycle | Step 6 (linear work production) | Step 4 (force generation, oscillatory work production) | Step 2 (CB detachment after ATP binding) |
The correlation between processes in sinusoidal analysis and phases in step analysis is theoretically perfect (Kawai and Brandt, 1980), and the measurements are carried out under the isometric condition. The correlation between tension transients and length transients is only approximate. The general rule is that a fast process in one method corresponds to a fast process in the other, but the correlation becomes weaker as the process becomes slower. The polarity of process A (phase 4) and process C (phase 2) is positive, and the polarity of process B (phase 3) is negative (Eq. 4). For the length transients, terminology used previously (Caremani et al., 2013) is employed.