| Technique . | Principle . | Applications in spines and synapses . |
|---|---|---|
| FRAP | Fluorescent molecules in a small region are photobleached, and subsequent movement of surrounding nonbleached fluorescent molecules into the photobleached area is monitored (Lippincott-Schwartz et al., 2018). | Svoboda et al., 1996; Bloodgood and Sabatini, 2005 |
| FCS | Fluctuation of fluorescence intensity from the detection volume fixed at a specific position is recorded, and a temporal correlation is analyzed (Elson, 2011). | Chen et al., 2015; Obashi et al., 2019 |
| RICS | Spatial correlation is analyzed from raster-scanned images (Digman and Gratton, 2011). | Obashi et al., 2019 |
| SPT | The movement of a single particle is tracked using time-lapse imaging, and a trajectory is made and analyzed. To detect single particles, the density of fluorescence particles should be kept low (Shen et al., 2017). | Borgdorff and Choquet, 2002; Varela et al., 2016 |
| SPT-PALM | Only a small number of photoactivatable fluorescent proteins in the field of view are activated and tracked until they are bleached (Manley et al., 2010). | Frost et al., 2010b; Nair et al., 2013 |
| Technique . | Principle . | Applications in spines and synapses . |
|---|---|---|
| FRAP | Fluorescent molecules in a small region are photobleached, and subsequent movement of surrounding nonbleached fluorescent molecules into the photobleached area is monitored (Lippincott-Schwartz et al., 2018). | Svoboda et al., 1996; Bloodgood and Sabatini, 2005 |
| FCS | Fluctuation of fluorescence intensity from the detection volume fixed at a specific position is recorded, and a temporal correlation is analyzed (Elson, 2011). | Chen et al., 2015; Obashi et al., 2019 |
| RICS | Spatial correlation is analyzed from raster-scanned images (Digman and Gratton, 2011). | Obashi et al., 2019 |
| SPT | The movement of a single particle is tracked using time-lapse imaging, and a trajectory is made and analyzed. To detect single particles, the density of fluorescence particles should be kept low (Shen et al., 2017). | Borgdorff and Choquet, 2002; Varela et al., 2016 |
| SPT-PALM | Only a small number of photoactivatable fluorescent proteins in the field of view are activated and tracked until they are bleached (Manley et al., 2010). | Frost et al., 2010b; Nair et al., 2013 |