Kinetic parameters for binding of Cm28 to KV1.2 and KV1.3 channels
| . | Mean ± SEM . | |
|---|---|---|
| KV1.2 . | KV1.3 . | |
| kon (nM−1 s−1) | 0.0011 ± 7.17 × 10−5 | 0.0013 ± 2.63 × 10−5 |
| koff (s−1) | 0.0013 ± 2.94 × 10−5 | 0.0028 ± 2.15 × 10−4 |
| Kd (nM) | 1.18 ± 0.047 | 2.15 ± 0.12 |
| n | 4 | 4 |
| . | Mean ± SEM . | |
|---|---|---|
| KV1.2 . | KV1.3 . | |
| kon (nM−1 s−1) | 0.0011 ± 7.17 × 10−5 | 0.0013 ± 2.63 × 10−5 |
| koff (s−1) | 0.0013 ± 2.94 × 10−5 | 0.0028 ± 2.15 × 10−4 |
| Kd (nM) | 1.18 ± 0.047 | 2.15 ± 0.12 |
| n | 4 | 4 |
For KV1.2, the kon and koff were calculated from time constants (τon, τoff) for the onset and recovery of the block in the presence of 2 nM Cm28. For KV1.3, kon and koff were determined by the fitting the 1/τon and 1/τoff values with linear regression function (Fig. 5 F, see Results for details). Kd was determined as koff/kon.