Table 1. Summary of steady-state ATPase... | Rockefeller University Press

Table 1.

Summary of steady-state ATPase results for human M2β-S1 exchanged with K104E versus WT RLC

Steady-state ATPase values (± SD), n = 3	WT	K104E	WT Non-ex
v₀ (s^–1)	0.02 ± 0.02	0.04 ± 0.04	0.02 ± 0.01
k_cat (s^–1)	4.5 ± 0.6	5.4 ± 0.8	5.4 ± 1.3
K_ATPase (µM)	49 ± 11	57 ± 14	53 ± 22

Steady-state ATPase values (± SD), n = 3	WT	K104E	WT Non-ex
v₀ (s^–1)	0.02 ± 0.02	0.04 ± 0.04	0.02 ± 0.01
k_cat (s^–1)	4.5 ± 0.6	5.4 ± 0.8	5.4 ± 1.3
K_ATPase (µM)	49 ± 11	57 ± 14	53 ± 22

K_ATPase, the actin concentration at which the ATPase rate is one half maximal actin-activated ATPase activity; k_cat, maximal actin-activated ATPase activity; v₀, absence of actin; Non-ex, non-exchanged myosin with endogenous RLC.

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