Time constants from exponential fits to dwell time histograms
| Fitted dwell time components (τ) in μs | CTZ n = 7 | CII n = 5 | (R, R)-2b n = 3 | CII + (R, R)-2b n = 6 |
|---|---|---|---|---|
| Closed | ||||
| τ1 | 300 ± 90 | 260 ± 50 | 170 ± 20 | 540 ± 210 |
| τ2 | 2,400 ± 1,400 | 1,500 ± 300 | 1,080 ± 70 | 5,800 ± 4,200 (5) |
| A1 | 89 ± 7% | 95 ± 2% | 91 ± 8% | 89 ± 6% |
| Open 1 | ||||
| τ1 | 240 ± 20 | 240 ± 40 | 140 ± 20 | 180 ± 50 |
| τ2 | 600 ± 300 (2) | — | 400 ± 50 | 480 ± 320 (2) |
| A1 | 92 ± 6% | 100% | 95 ± 5% | 93 ± 8% |
| Open 2 | ||||
| τ1 | 280 ± 30 | 200 ± 10 | 220 ± 60 | 230 ± 40 |
| τ2 | 570 (1) | — | — | 400 ± 140 (3) |
| A1 | 97 ± 3% | 100% | 100% | 86 ± 13% |
| Open 3 | ||||
| τ1 | 260 ± 20 | 170 ± 20 | 180 ± 80 | 220 ± 50 |
| τ2 | 700 (1) | 330 ± 30 (3) | 240 (1) | — |
| A1 | 99 ± 1% | 85 ± 14% | 86 ± 17% | 100% |
| Open 4 | ||||
| τ1 | 320 ± 70 | 180 ± 40 | 250 ± 80 | 270 ± 50 (5) |
| τ2 | 1,200 ± 400 (6) | — | 900 ± 300 | 2,500 ± 1,800 (4) |
| A1 | 87 ± 4% | 100% | 75 ± 11% | 98 ± 1% |
| Fitted dwell time components (τ) in μs | CTZ | CII | (R, R)-2b | CII + (R, R)-2b |
|---|---|---|---|---|
| Closed | ||||
| τ1 | 300 ± 90 | 260 ± 50 | 170 ± 20 | 540 ± 210 |
| τ2 | 2,400 ± 1,400 | 1,500 ± 300 | 1,080 ± 70 | 5,800 ± 4,200 (5) |
| | 89 ± 7% | 95 ± 2% | 91 ± 8% | 89 ± 6% |
| Open 1 | ||||
| τ1 | 240 ± 20 | 240 ± 40 | 140 ± 20 | 180 ± 50 |
| τ2 | 600 ± 300 (2) | — | 400 ± 50 | 480 ± 320 (2) |
| | 92 ± 6% | 100% | 95 ± 5% | 93 ± 8% |
| Open 2 | ||||
| τ1 | 280 ± 30 | 200 ± 10 | 220 ± 60 | 230 ± 40 |
| τ2 | 570 (1) | — | — | 400 ± 140 (3) |
| | 97 ± 3% | 100% | 100% | 86 ± 13% |
| Open 3 | ||||
| τ1 | 260 ± 20 | 170 ± 20 | 180 ± 80 | 220 ± 50 |
| τ2 | 700 (1) | 330 ± 30 (3) | 240 (1) | — |
| | 99 ± 1% | 85 ± 14% | 86 ± 17% | 100% |
| Open 4 | ||||
| τ1 | 320 ± 70 | 180 ± 40 | 250 ± 80 | 270 ± 50 (5) |
| τ2 | 1,200 ± 400 (6) | — | 900 ± 300 | 2,500 ± 1,800 (4) |
| | 87 ± 4% | 100% | 75 ± 11% | 98 ± 1% |
Single-channel recordings of GluA2 wild type receptors were obtained in glutamate (10 mM) and the following positive allosteric modulators: CTZ (100 µM), CII toxin (100–500 nM), (R, R)-2b (10 μM), or CII + (R, R)-2b. Histograms of dwell times of closed and open levels (1–4) were obtained from idealized single-channel traces and fit with one or two exponential components (Fig. 7; τ1 and τ2). The amplitude of the first component (A1) is expressed in percent where appropriate, and n is the number of patches. Where only a subset of patches in the group could be fit, the number of patches in this subset used to obtain the time constant is given in parentheses.
Sharing content requires targeting cookies to be enabled. Please update your cookie preferences to use this feature.