Steady-state activation and inactivation parameters of ICaL in the absence and presence of 10 µM phenanthrene (Phe)
Activation
Inactivation
Control
10 µM Phe
Control
10 µM Phe
V0.5
−23.6 ± 1.3 mV
−24.9 ± 1.1 mV
−31.8 ± 0.1 mV
−32.0 ± 0.1 mV
k
5.1 ± 0.2
5.1 ± 0.2
1.4 ± 0.2
1.1 ± 0.1
Activation
Inactivation
Control
10 µM Phe
Control
10 µM Phe
V0.5
−23.6 ± 1.3 mV
−24.9 ± 1.1 mV
−31.8 ± 0.1 mV
−32.0 ± 0.1 mV
k
5.1 ± 0.2
5.1 ± 0.2
1.4 ± 0.2
1.1 ± 0.1
Parameters were obtained by fitting with Boltzman equation of individual activation (G/Gmax) and inactivation (I/Imax) curves as described in Materials and methods. V0.5, half-maximal voltage of activation and inactivation; k, slope factor. All values are presented as means ± SEM (n = 6; N = 2).