| # . | Site . | Compound . | Mutation . | Length (ns) . |
|---|---|---|---|---|
| 1 | S3/S4 | Wu50 | WT | 1,000 |
| 2 | S3/S4 | Wu50 | 2R = M356R/A359R | 500 |
| 3 | S4/pore | Wu50 | WT | 500 |
| 4 | S4/pore | Wu50 | W454A | 500 |
| 5 | S4/pore | Wu50 | R362Q | 500 |
| 6 | S4/pore | Wu50 | W454A/R362Q | 1,000 |
| 7 | S4/pore | Wu50 | R362Q/R365Q | 1,000 |
| 8 | S4/pore | Wu50 | R362Q/R365Q/W454A | 640 |
| 9 | S3/S4 | Wu161 | WT | 500 |
| 10 | S4/pore | Wu161 | WT | 500 |
| 11 | Top-VSD | Wu50 | WT | 500 |
| # . | Site . | Compound . | Mutation . | Length (ns) . |
|---|---|---|---|---|
| 1 | S3/S4 | Wu50 | WT | 1,000 |
| 2 | S3/S4 | Wu50 | 2R = M356R/A359R | 500 |
| 3 | S4/pore | Wu50 | WT | 500 |
| 4 | S4/pore | Wu50 | W454A | 500 |
| 5 | S4/pore | Wu50 | R362Q | 500 |
| 6 | S4/pore | Wu50 | W454A/R362Q | 1,000 |
| 7 | S4/pore | Wu50 | R362Q/R365Q | 1,000 |
| 8 | S4/pore | Wu50 | R362Q/R365Q/W454A | 640 |
| 9 | S3/S4 | Wu161 | WT | 500 |
| 10 | S4/pore | Wu161 | WT | 500 |
| 11 | Top-VSD | Wu50 | WT | 500 |