| Channel subunit . | Vrest (mV) . | Vrev (mV) . | ΔVrev (mV) . | 95% CI (mV) . | P . | Current, I (µA) . | ΔI (µA) . | 95% CI (µA) . | P . |
|---|---|---|---|---|---|---|---|---|---|
| . | Mean ± SEM (n) . | Mean ± SEM (n) . | . | (min, max) . | . | Mean ± SEM (n) . | . | (min, max) . | . |
| MEC-4d | +13.61 ± 0.2 (95) | −1.85 ± 0.114 (93) | 47.20 | (38.4, 54.7) | 2.38e-25 | −10.81 ± 0.085 (95) | −10.7 | (−12.4 to −9.14) | 4.53E-39 |
| UNC-8d | +6.93 ± 0.16 (56) | −3.67 ± 0.163 (59) | 52.80 | (44.2, 60.0) | 2.85e-20 | −1.68 ± 0.017 (59) | −1.57 | (−1.83 to −1.33) | 1.15E-27 |
| DEGT-1d | −19.31 ± 0.1 (109) | −23.31 ± 0.140 (111) | 25.80 | (17.2, 33.3) | 9.05e-13 | −0.61 ± 0.004 (113) | −0.50 | (−0.58 to −0.42) | 3.08e-34 |
| DEGT-1d alone | −20.87 ± 3.1 (15) | −23.99 ± 0.717 (15) | 30.8 | (21.8, 40.5) | 6.56e-05 | −0.53 ± 0.019 (15) | −0.419 | (−0.588 to −0.298) | 5.23e-09 |
| DEL-1d | −38 0.94 ± 0.9 (18) | −60.68 ± 1.563 (19) | −11.60 | (−26.0, 4.43) | 0.166 | −0.16 ± 0.008 (20) | −0.04 | (−0.13 to 0.02) | 0.239 |
| MEC-10d | −27.31 ± 0.92 (19) | −42.12 ± 1.089 (19) | 6.96 | (−5.24, 18.7) | 0.251 | −0.21 ± 0.009 (19) | −0.09 | (−0.19 to −0.03) | 0.004 |
| MEC-2 alone | −48 ± 7.7 (11) | −67.28 ± 1.686 (11) | −12.5 | (−24.8, 1.2) | 0.162 | −0.11 ± 0.004 (11) | −0.000626 | (−0.0325 to 0.0257) | 0.236 |
| Uninjected | −36 0.22 ± 0.12 (143) | −49.08 ± 0.396 (95) | - | - | - | −0.12 ± 0.001 (144) | - | - | - |
| Channel subunit . | Vrest (mV) . | Vrev (mV) . | ΔVrev (mV) . | 95% CI (mV) . | P . | Current, I (µA) . | ΔI (µA) . | 95% CI (µA) . | P . |
|---|---|---|---|---|---|---|---|---|---|
| . | Mean ± SEM (n) . | Mean ± SEM (n) . | . | (min, max) . | . | Mean ± SEM (n) . | . | (min, max) . | . |
| MEC-4d | +13.61 ± 0.2 (95) | −1.85 ± 0.114 (93) | 47.20 | (38.4, 54.7) | 2.38e-25 | −10.81 ± 0.085 (95) | −10.7 | (−12.4 to −9.14) | 4.53E-39 |
| UNC-8d | +6.93 ± 0.16 (56) | −3.67 ± 0.163 (59) | 52.80 | (44.2, 60.0) | 2.85e-20 | −1.68 ± 0.017 (59) | −1.57 | (−1.83 to −1.33) | 1.15E-27 |
| DEGT-1d | −19.31 ± 0.1 (109) | −23.31 ± 0.140 (111) | 25.80 | (17.2, 33.3) | 9.05e-13 | −0.61 ± 0.004 (113) | −0.50 | (−0.58 to −0.42) | 3.08e-34 |
| DEGT-1d alone | −20.87 ± 3.1 (15) | −23.99 ± 0.717 (15) | 30.8 | (21.8, 40.5) | 6.56e-05 | −0.53 ± 0.019 (15) | −0.419 | (−0.588 to −0.298) | 5.23e-09 |
| DEL-1d | −38 0.94 ± 0.9 (18) | −60.68 ± 1.563 (19) | −11.60 | (−26.0, 4.43) | 0.166 | −0.16 ± 0.008 (20) | −0.04 | (−0.13 to 0.02) | 0.239 |
| MEC-10d | −27.31 ± 0.92 (19) | −42.12 ± 1.089 (19) | 6.96 | (−5.24, 18.7) | 0.251 | −0.21 ± 0.009 (19) | −0.09 | (−0.19 to −0.03) | 0.004 |
| MEC-2 alone | −48 ± 7.7 (11) | −67.28 ± 1.686 (11) | −12.5 | (−24.8, 1.2) | 0.162 | −0.11 ± 0.004 (11) | −0.000626 | (−0.0325 to 0.0257) | 0.236 |
| Uninjected | −36 0.22 ± 0.12 (143) | −49.08 ± 0.396 (95) | - | - | - | −0.12 ± 0.001 (144) | - | - | - |
Mean ± SEM for the resting membrane voltage (Vrest) under current clamp conditions and reversal potential (Vrev) and current (current, I) under voltage clamp conditions at the beginning of each recording session. Vrev was calculated from the ramp protocol in Fig. 1 A, and current, I, was measured at −85 mV. Numbers in parentheses are the total number of oocytes analyzed from at least three donor frogs. Confidence intervals (CIs) are derived from estimation statistics to indicate the effect of channel expression on reversal potential (ΔVrev) and current (ΔI), and exact P values from Mann–Whitney test indicate which conditions generated currents that differed from uninjected control oocytes. Except for MEC‑2 and DEGT‑1d alone (shown in Fig. S1), these data are drawn from the same data set displayed in graphical form in the lower panels of Fig. 1, I and J.